Table 3 Example of uncorrected Ct values characteristic to the reference (housekeeping) primer G6PDH. Exemplification of the uncorrected Ct (average of three replicates) values characteristic to the reference (housekeeping) primer G6PDH of the DNA samples under study. Although starting concentration of DNA for all samples was 10 ng the Ct values for the housekeeping genes differed slightly from patient to patient and from group to group.
From: A method for accurate detection of genomic microdeletions using real-time quantitative PCR
Samples | G6PDH Ct value | |
|---|---|---|
Control | 1 | 21.2196 |
| Â | 2 | 21.1595 |
| Â | 3 | 23.0891 |
| Â | 4 | 21.3748 |
Group 1 | 1 | 19.8799 |
| Â | 2 | 21.2899 |
| Â | 3 | 20.5799 |
| Â | 4 | 20.7859 |
| Â | 5 | 21.4781 |
| Â | 6 | 19.5612 |
Group 2 | 1 | 20.3756 |
| Â | 2 | 21.1237 |
| Â | 3 | 21.1544 |
| Â | 4 | 21.1484 |
| Â | 5 | 20.5768 |
| Â | 6 | 19.9122 |
22qDuplication | 1 | 21.3409 |