Comparison of gene expression signatures of diamide, H2O2 and menadione exposed Aspergillus nidulans cultures – linking genome-wide transcriptional changes to cellular physiology
© Pócsi et al; licensee BioMed Central Ltd. 2005
Received: 23 September 2005
Accepted: 20 December 2005
Published: 20 December 2005
In addition to their cytotoxic nature, reactive oxygen species (ROS) are also signal molecules in diverse cellular processes in eukaryotic organisms. Linking genome-wide transcriptional changes to cellular physiology in oxidative stress-exposed Aspergillus nidulans cultures provides the opportunity to estimate the sizes of peroxide (O22-), superoxide (O2•-) and glutathione/glutathione disulphide (GSH/GSSG) redox imbalance responses.
Genome-wide transcriptional changes triggered by diamide, H2O2 and menadione in A. nidulans vegetative tissues were recorded using DNA microarrays containing 3533 unique PCR-amplified probes. Evaluation of LOESS-normalized data indicated that 2499 gene probes were affected by at least one stress-inducing agent. The stress induced by diamide and H2O2 were pulse-like, with recovery after 1 h exposure time while no recovery was observed with menadione. The distribution of stress-responsive gene probes among major physiological functional categories was approximately the same for each agent. The gene group sizes solely responsive to changes in intracellular O22-, O2•- concentrations or to GSH/GSSG redox imbalance were estimated at 7.7, 32.6 and 13.0 %, respectively. Gene groups responsive to diamide, H2O2 and menadione treatments and gene groups influenced by GSH/GSSG, O22- and O2•- were only partly overlapping with distinct enrichment profiles within functional categories. Changes in the GSH/GSSG redox state influenced expression of genes coding for PBS2 like MAPK kinase homologue, PSK2 kinase homologue, AtfA transcription factor, and many elements of ubiquitin tagging, cell division cycle regulators, translation machinery proteins, defense and stress proteins, transport proteins as well as many enzymes of the primary and secondary metabolisms. Meanwhile, a separate set of genes encoding transport proteins, CpcA and JlbA amino acid starvation-responsive transcription factors, and some elements of sexual development and sporulation was ROS responsive.
The existence of separate O22-, O2•- and GSH/GSSG responsive gene groups in a eukaryotic genome has been demonstrated. Oxidant-triggered, genome-wide transcriptional changes should be analyzed considering changes in oxidative stress-responsive physiological conditions and not correlating them directly to the chemistry and concentrations of the oxidative stress-inducing agent.
There is experimental evidence that formation of oxidants is regulated in cells by factors, affecting targets specifically [1–4]. Different oxidative stress-inducing agents (cumene hydroperoxide, diamide, H2O2, linoleic acid 13-hydroperoxide, menadione), which disturb intracellular oxidant concentrations specifically, influence segments of the genome differentially with almost no overlap in Saccharomyces cerevisiae, when a genome-wide set of deletion strains was tested . For example, respiration was shown to be important in H2O2 defense while the NADPH-producing pentose phosphate pathway was menadione-responsive .
Oxidant levels are clearly influenced by the chemical structure of the agents used to trigger stress as well as the concentration and exposure time of the agents [6, 7]. Overdosing with reactants decreases specificity of treatments by increasing the number of affected oxidants and diminishing survival rates [6, 7].
The filamentous fungus Aspergillus nidulans with whole genome sequence availability and extensive EST collections is an excellent model to perform transcriptome analyses using EST-based DNA microarrays [8–10]. Physiological parameters indicative of oxidative stress can also be measured with high reproducibility in A. nidulans [11, 12].
In this study, we assay physiologically well-defined oxidative stress-inducing systems to describe the global transcriptional changes observable in A. nidulans vegetative hyphae exposed to diamide, H2O2 and menadione. In addition, we demonstrate large gene groups responsive to changes in intracellular peroxide (O22-) and superoxide (O2•-) levels or glutathione/glutathione disulfide (GSH/GSSG) redox imbalance.
Optimization of oxidative stress inducing conditions
For diamide, H2O2 and menadione treatments, 1.8, 75 and 0.8 mM were selected, respectively, which were well below the "dosis lethalis minima" (DLM) determined in liquid A. nidulans cultures (Additional file 1:Supplement1 for physiological changes).
Short time (1 h) exposures to 1.8 mM diamide increased the specific glutathione S-transferase (GST) and catalase activities while superoxide dismutase (SOD) activity went up only after extended treatments (6 h; Additional file 1:Supplement1 for physiological changes). The specific SOD, GST and catalase activities all responded to short 0.8 mM menadione treatments (1 h) while 75 mM H2O2 triggered elevations only in catalase activity (Additional file 1:Supplement1 for physiological changes). After extended (6 h) incubation with H2O2, the increase in SOD and GST activities was significant at P < 5 % and P < 1 %, respectively (Additional file 1:Supplement1 for physiological changes).
Similar to previous observations [6, 7], 1.8 mM diamide, 75 mM H2O2 and 0.8 mM menadione did not influence cell survival rates significantly even after extended (9 h) treatment periods (data not shown).
Gene activation or repression was dose-dependent when diamide and menadione concentrations were elevated from 1.0 to 1.8 mM and from 0.35 to 0.8 mM, respectively (Figure 2). When the concentration of H2O2 was elevated from 75 to 150 mM the mRNA pools were degraded at 1 and 3 h exposure times while there was apparently no reduction in rRNA levels at any exposure time tested (Figure 2).
Evaluation of cDNA microarray gene expression data
Time-dependence of gene expression profiles from cDNA microarrays showed that gstA was up-regulated by menadione and diamide while sodA was only induced by menadione (Figures 2E and 2F). The transcription of sodA was not fluctuating in the presence of H2O2 and diamide, and all M' values were within [+1,-1] thresholds (Figure 2F). The expression of Ca2+-calmodulin-dependent serine-threonine-protein kinase was clearly fluctuating in microarray experiments with a maximum down-regulation at 1 h incubation and with a maximum induction of 3 h incubation under diamide and H2O2-treatments (Figure 2G).
Finally, in addition to Additional file 2:Supplement2, for the list of oxidative stress responsive gene probes, the full list of oxidative stress responsive gene probes is available at National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO)  on Platforms GPL1752 and GPL1756. Oxidative stress sensitive genes equally responsive to diamide, H2O2 and menadione treatments, i.e. the likely GSH/GSSG-responsive genes, are briefly summarized in Additional file 5:Supplement5 for a selection of GSH/GSSG responsive genes. A selection of genes considered as ROS (superoxide or peroxide) responsive is given in Additional file 6:Supplement6 for a selection of ROS responsive genes.
ROS-generating chemicals are frequently used as agents to induce antioxidative defense systems and to study the mechanism of adaptation to oxidative stress . Although organisms might respond to naturally occurring oxidative stress conditions  or endogenous ROS  in a quite different way these reactants are highly suitable to optimize experimental conditions and, hence, to improve the reproducibility of the size and intensity of stress sensed by cells [6, 7]. Without fine-tuning experimental conditions, e.g. optimizing the quantities of oxidant added, these chemicals have been shown to affect the concentrations of multiple types of ROS and trigger concomitantly severe GSH/GSSG redox imbalance .
Among the oxidative stress-inducing agents selected, diamide is a thiol-oxidizing agent resulting in fast oxidation of GSH to GSSG resulting in GSH/GSSG redox imbalance , H2O2 increases intracellular peroxide (O22-) levels, which leads to the direct oxidation of the sulfur-containing amino acids and the generation of OH• radicals , menadione generates superoxide anions (O2•-) in a redox cycle, which destroys 4Fe-4S proteins productng of OH• . Menadione may also affect GSH pool directly via a detoxification reaction catalyzed by GST .
Due to distinct mechanism of action, these chemicals provide the opportunity to map GSH/GSSG, O22- and O2•--responsive sets of genes within the A. nidulans genome. In yeast, there is literature supporting the assumption that these agents influence different elements of the oxidative stress response [5, 19–29].
In the β-lactam producer P. chrysogenum, diamide, H2O2, tert-butyl hydroperoxide and menadione were used to set up systems where intracellular GSH/GSSG redox balance as well as intracellular peroxide and superoxide levels were altered selectively without influencing the other two physiological parameters [6, 7].
Unfortunately, such clear-cut experimental conditions could only be set up using diamide in A. nidulans, which, when employed in a concentration of 1.8 mM, disturbed only GSH/GSSG redox balances without increasing intracellular ROS concentrations (Figure 1, Additional file 1:Supplement1 for physiological changes). H2O2 and menadione always affected the GSH/GSSG redox state of the cells, and menadione also influenced intracellular peroxide levels in addition to superoxide concentrations (Figure 1, Additional file 1:Supplement1 for physiological changes). This can be explained with the 6-17-fold lower specific catalase activities detected in stress-exposed A. nidulans cells in comparison to P. chrysogenum cultures (Additional file 1 :Supplement1 for physiological changes) . The relatively weak peroxide-decomposing capability of A. nidulans explains the disturbance of GSH/GSSG redox balance with H2O2 and menadione, because GSH is also oxidized non-enzymatically by many ROS including OH•, a free radical known to be generated under these treatments [14, 18].
Because H2O2 and menadione influenced the GSH/GSSG redox couple and intracellular ROS levels in an inseparable manner 0.8 mM and 75 mM concentrations were selected, respectively, for DNA microarray experiments taking into consideration cell survival and gene expression data (Additional file 1:Supplement1 for physiological changes, Figure 2). The specific activities of selected antioxidative enzymes were increased (Additional file 1:Supplement1 for physiological changes) and cell survival rates were not affected significantly by them (data not shown), and no deterioration of the mRNA and rRNA populations were observed under these conditions (Figure 2A). Stress-inducing agents employed in these selected concentrations triggered detectable transcriptional changes in cDNA microarray experiments as visualized on Northern blots (Figure 2A).
Similarly to P. chrysogenum [6, 7], there was a correlation between intracellular GSH/GSSG values, ROS levels and increase in specific antioxidative enzyme activities in A. nidulans (Additional file 1:Supplement1 for physiological changes). For example, diamide failed to induce SOD activity (O2•--responsive) and H2O2 did not influence SOD (O2•--responsive) and GST (catalyses GSH-dependent reaction) activities after 1 h exposure (Additional file 1:Supplement1 for physiological changes). As shown in Figure 2, the selected stress-inducing agent concentrations also allowed us to record ROS and GSH/GSSG specific differences at the level of gene transcripts.
Normalized DNA microarray data correlated with transcriptional changes determined on Northern blots (Figure 3). Because the calculation of both M and MNorthern values included division of gene expression data recorded in stress-exposed cultures with their counterparts found in controls the non-stress-related incubation-time-dependent changes taking place in control cultures distorted these parameters and, hence, disturbed their correlation especially at longer incubation periods (Figures 2C, 2D, 2F and 2G). M' = f(t) functions for individual gene probes were fluctuating in many cases but these tendencies might represent either real fluctuations like in the case of the homologue of the Ca2+-calmodulin-dependent serine-threonine-protein kinase (Figures 2A and 2G) or virtual fluctuations – a result of non-stress-related changes in controls (Figure 2C). Taking into consideration the high frequency of fluctuating gene expression patterns we considered only transcriptional changes above or below the [+1;-1] M' thresholds as a function of incubation time during gene function analysis (Figure 6, Additional file 2:Supplement2 for the list of oxidative stress responsive gene probes, Additional file 3:Supplement3 for the list of gene probes considered in significant enrichment calculations, Additional file 5:Supplement5 for a selection of GSH/GSSG responsive genes, Additional file 6:Supplement6 for a selection of ROS responsive genes).
All three chemicals used to trigger oxidative stress induced a genome-wide transcriptional stress response, which reached its maximum at 1 h incubation time as indicated by the number of gene probes affected more than two-fold by oxidative stress (Figure 4). The induction of antioxidative enzymes by H2O2 is regarded in general faster than that triggered by menadione as exemplified by the transcriptional changes recorded for Cu,Zn-containing superoxide dismutase in the yeast S. pombe  and for the sodA gene in this study (more than two-fold up-regulation was observable between 3–6 h incubations in menadione-treated cultures, Figures 2A, 2C and 2F). In agreement with this, the kinetics of intracellular accumulation of ROS and decrease in GSH/GSSG redox ratio was slow although steady in the presence of 0.8 mM menadione (Figure 1). Nevertheless, up-regulation of gstA, which plays a predominant role in detoxification of menadione , as early as 30 min (Figure 3A) was indicative of an early influx of this agent into cells. Menadione is also an effective arylating agent in addition to its redox-cycling behavior and, especially at high concentrations (higher than 0.2 mM), it enhances membrane fluidity . Oxidative and non-oxidative cell injuries caused by menadione are likely to contribute to short-term but large-scale transcriptional response observed in menadione-treated cultures (Figure 4). The transient decrease in the ratio of stress-affected gene-probes at 3–6 h exposure times and the appearance of a second maximum at 9 h (Figure 4) may be indicative of a shift from a mixed oxidative/non-oxidative towards a pure oxidative stress response in the gene expression signature of menadione.
Diamide, H2O2 and menadione all influenced large groups of genes within the genome with considerable overlap (Figures 5 and 6). The appearance of large groups of gene probes equally responsive to diamide, H2O2, menadione (likely GSH/GSSG-dependent regulation), or to H2O2 and menadione but not to diamide (likely O22--dependent regulation) or solely responsive to menadione (likely O2•--dependent regulation) highly supported the hypothesis that separate ROS and GSH/GSSG redox state regulated groups of genes are present in a genome. The ratios of O22--, O2•-- and GSH/GSSG responsive genes were estimated at 7.7, 32.6 and 13.0 %, respectively (Figure 5).
The ratio of O2•--responsive gene probes was likely overestimated to an unknown extent by counting genes that were induced or repressed exclusively by non-oxidative types of stress also triggered by 0.8 mM menadione . Moreover, one must consider that the oxidative stress-inducing agents used in this study may also disturb physiological parameters other than O2•-, O22-, GSH and GSSG concentrations, e.g. the production of lipid peroxides [31, 32], and these changes may also have significant impact on global gene expression patterns as indicated by the relatively high ratios of only H2O2 (6.3 %), only diamide (8.4 %), diamide and menadione and but not H2O2 (11.8 %; e.g. gstA; Figures 2B and 2E, Additional file 2:Supplement2 for the list of oxidative stress responsive gene probes) and H2O2 and diamide but not menadione (2.6 %)responsive gene probes (Figure 5). In S. cerevisiae, cells exposed to the toxic lipid peroxidation product linoleic acid hydroperoxide (LoaOOH), a distinct set of genes was stimulated that were not responsive to other oxidants or heat shock . The frequent appearance of gene probes responding to different stress-inducing agents with a more than two-fold change in transcription but in the opposite way (17.6 % in total) may also be the consequence of the effects of either not-yet-identified physiological factors or non-systematic errors of DNA microarray hybridizations. All these factors may further distort the ratios calculated for O22--, O2•-- and GSH/GSSG-responsive gene probes as well.
Gene probe distribution analysis (Figures 6 and 7) and significant enrichment calculations (Figure 7, Additional file 3:Supplement3 for the list of gene probes considered in significant enrichment calculations, Additional file 4:Supplement4 for the results of significant enrichment calculations) indicated that the distribution of stress responsive gene probes between functional categories and their enrichment profiles were different and only partially overlapping when grouped according to their responsiveness to stress-inducing agents or to changes in physiological parameters. This finding indicates that genome-wide transcriptional changes should be analyzed by correlating them directly to changes in oxidative stress-response physiological parameters and not to the chemistry and concentration of oxidant used to trigger stress.
According to Shafer and Buettner , the GSH/GSSG redox couple plays a pivotal role in controlling thiol and disulfide nano-switches that move cells from proliferation, through various stages of differentiation and, when the redox environment of the cells cannot be maintained, towards apoptosis. In accordance with this, our observations reflect the distinguished role of the GSH/GSSG redox state in genome-wide oxidative stress response (Additional file 5:Supplement5 for a selection of GSH/GSSG responsive genes). For example, many components of signal transduction pathways, including homologues of PBS2 like MAPK kinase (related to the S. cerevisiae PBS2 and the S. pombe Wis1 MAPK kinases, which phosphorylate HOG1 and Spc1-Sty1 MAPKs, respectively) , PSK2 kinase , important transcription factors (e.g. AtfA, a homologue of the S. pombe Atf1 [27, 35, 36]), ubiquitin tagging, cell cycle regulators, translation machinery proteins, defense and stress proteins (e.g. glutathione peroxidase, trehalose synthase), transport proteins (e.g. P-type Na+-ATPase) as well as many enzymes of the primary (e.g. glycolytic enzymes) and secondary (e.g. sterigmatocystin biosynthetic enzymes) metabolism, seemed to be responsive to GSH/GSSG redox imbalances at the level of transcription (Additional file 5:Supplement5 for a selection of GSH/GSSG responsive genes).
The stress-activated protein kinase (SAPK) signal transduction pathway (MAPK Spc1-Sty1-dependent signal transduction) and the bZip transcriptional factor Atf1 have been shown to play a pivotal role in the regulation of the core environmental stress response (CESR) in S. pombe, which is common to all stresses including oxidative stress . The stress-responsive genes controlled by Atf1 in S. pombe include e.g. gpx1 glutathione peroxidase (oxidative, osmotic and thermal stress) , tps1 trehalose-6-phosphate synthase (oxidative and salt-induced osmotic stress)  and cta3 cation-transporting P-type ATPase (salt-induced osmotic stress) , and atf1 itself is an induced CESR gene . Based on functional and sequence homologies as well as gene expression changes presented in Additional file 5:Supplement5 for a selection of GSH/GSSG responsive genes, the existence of an oxidative stress responsive SAPK-AtfA regulatory pathway with a similar set of target genes is likely in A. nidulans as hypothesized by Aguirre et al. . But this pathway seems to be controlled primarily by changes in the GSH/GSSG redox balance instead of ROS (peroxide) concentrations .
The GSH/GSSG-dependent down-regulation of ribosomal proteins located in the cytoplasm of oxidative stress-exposed A. nidulans cells (Additional file 5:Supplement5 for a selection of GSH/GSSG responsive genes) may result in a decrease in the rate of protein synthesis similar to LoaOOH-exposed S. cerevisiae cells . Genes encoding ribosomal proteins located in the mitochondria was induced (Additional file 5:Supplement5 for a selection of GSH/GSSG responsive genes) indicating an increased protein synthesis in these organelles.
GSH/GSSG redox imbalances affect glycolysis at 6-phosphofructo-2-kinase (induction) and fructose biphosphate aldolase (repression), which may result in the intracellular accumulation of fructose-1,6-bisphosphate. This glycolytic metabolite is known to protect cells by improving energy status and mitochondrial functions in mammals [39, 40]. In accordance with the findings published by Jayashree and Subramanyam , the synthesis of the mycotoxin sterigmatocystin (a precursor of aflatoxin B1 in aflatoxin-producing fungi) is regulated positively by oxidative stress (Additional file 5:Supplement5 for a selection of GSH/GSSG responsive genes, Figure 7).
Large gene groups responsive to ROS but not to changes in the GSH/GSSG redox balance (Additional file 6:Supplement6 for a selection of ROS responsive genes) indicated that GSH-independent stress-sensing regulatory network(s) also operated in A. nidulans cells exposed to oxidative stress. O22- and O2•- species affected mainly negatively transport processes like nuclear export-import via the down-regulation of the ran/spi1 homologue [41, 42] and ntf2 nuclear transport factor 2 [42, 43] and the induction of the yeast GTR2 homologue, a negative regulator of the Ran G protein cycle  (Additional file 6:Supplement6 for a selection of ROS responsive genes). ROS also seem to influence signal transduction e.g. via. repression of the homologue of the Cryphonectria parasitica cppk1 protein kinase . At the level of transcription, CpcA-JlbA amino acid starvation-responsive transcription factors [46, 47] are likely to contribute substantially to the regulation of oxidative stress response against ROS (Additional file 6:Supplement6 for a selection of ROS responsive genes). Opposite to GSH/GSSG-dependent regulation (Additional file 5:Supplement5 for a selection of GSH/GSSG responsive genes), translation was hardly effected by ROS but the degradation of abnormal or damaged proteins in mitochondria by Lon protease  was induced by superoxide. Elements of sexual development and sporulation were found to be ROS-responsive, including a homologue of the meiosis inducer kinase IME2 (yeast) [49, 50], AspC septin, SpoC1-C1C conidium-specific protein  and a homologue of the cdc5 kinase [52, 53] (Additional file 6:Supplement6 for a selection of ROS responsive genes). The down-regulation of the latter kinase together with a blockage in the nuclear export-import system [41–44] and a superoxide-dependent repression of the benA hyphal tubulin gene  may lead to a cell cycle arrest in oxidative stress. H2O2 and menadione have been reported to cause G2 and G1 phase cell cycle arrests in S. cerevisiae, respectively , and several cell-cycle-regulated transcripts were also shown to be down-regulated in linoleic acid hydroperoxide-exposed S. cerevisiae cells .
In addition to the harmful effects on living cells, there is a growing appreciation of ROS as important signal molecules in diverse cellular processes [1, 4]. For example, ROS (superoxide, H2O2) generated endogenously by NADPH oxidases (NOXs) have been demonstrated to play a role in macrophage [56, 57] and insulin  signaling and regulation of vascular functions [4, 59] in mammals. NOX-generated ROS also regulates cell expansion in the plant Arabidopsis thaliana . NOX-generated ROS have also been shown to regulate the sexual development in A. nidulans [3, 61] and Podospora anserina  as well as ascospore germination in P. anserina  and N. crassa , which clearly points that ROS are likely involved in regulation of development and cell physiology in filamentous fungi as well.
Large and separate ROS (O22-, O2•-) and GSH/GSSG redox groups of genes are present in the A. nidulans genome. ROS and GSH/GSSG signaling seems to proceed independently of each other and may express their effects via different regulatory networks. Unless the experimental conditions are optimized oxidative stress-inducing agents are likely to influence many physiological parameters and a concomitant activation of these separate signal transduction and regulatory networks can be predicted to reach an appropriate stress response at the level of transcriptome.
Considering that diamide – GSH/GSSG, H2O2 – O22- and menadione – O2•- responsive sets of genes were only partly overlapping and their significant enrichment profiles in functional categories, we recommend that oxidative-stress-triggered global transcriptional changes should be evaluated by correlating them to changes in oxidative-stress-responsive physiological parameters. Correlating genome-wide transcriptional changes directly to the chemistry and concentrations of stress-inducing agents might lead to unsatisfactory conclusions unless the experimental conditions are thoroughly optimized and physiologically characterized.
DNA microarray data presented in this study demonstrate changes in the transcriptome of oxidative stress-exposed A. nidulans cells. A more direct connection of genome-wide transcriptional changes to cellular physiology will be reached by a thorough and integrative analysis of stress-provoked changes observable at the levels of proteome and metabolome , which is now in progress in our laboratory.
Strain, culture conditions, cellular physiology
A. nidulans FGSC 26 (biA1, veA1) was grown in shake flasks (500 ml) containing 100 ml minimal-nitrate medium, pH 6.5  supplemented with 0.5 % yeast extract. Culture media were inoculated with 1 × 108 spores and were incubated for 18 h at 37°C and 3.3 Hz shaking frequency. Mycelia were separated by filtration on sintered glass and were transferred immediately into pre-incubated 100 ml aliquots of minimal-nitrate medium, pH 6.5 either supplemented with stress-inducing agents (stress-exposed cultures) or left untreated (controls).
"Dosis lethalis minima" (DLM) values for diamide (azodicarboxylic acid bis [N,N-dimethylamide]), H2O2 and menadione (menadione sodium bisulfite, a water-soluble derivative of 2-methyl-1,4-naphthoquinone was used in all experiments ) were determined in the minimal-nitrate medium, supplemented with the oxidative stress-inducing agents in different concentrations (diamide: 1.0–3.0 mM; H2O2: 50–750 mM; menadione: 0.2–2.0 mM) and measuring cell survivals after 9 h treatments (37°C, 3.3 Hz shaking frequency). Cell survival rates were estimated after transferring stress-exposed mycelia into 100 ml aliquots of oxidant-free minimal-nitrate medium by monitoring the increase in the dry cell mass (DCM) for 24 hours (37°C, 3.3 Hz shaking frequency) [6, 7]. DLM was defined as the lowest oxidant concentration that resulted in a complete lack of growth of treated mycelia in the oxidant-free medium.
Changes in intracellular concentrations of reactive oxygen species (ROS; O2•-, O22-) and GSH/GSSG balance were optimized to keep the number of physiological parameters affected by either diamide, H2O2 or menadione triggered stress as low as possible [6, 7]. This goal was approached by exposing 18 h mycelia to these agents dissolved at several concentrations in the minimal-nitrate medium. Intracellular GSH, GSSG, peroxide and superoxide levels were determined at 15 min, 30 min, 1 h, 3 h, 6 h and 9 h exposure times.
For GSH and GSSG determinations, mycelia from 5–10 ml aliquots of both stress-exposed and control cultures were filtered out and washed with ice-cold sterile distilled water. Mycelial mats were re-suspended in ice-cold 5 % (w/v) 5-sulfosalicylic acid by vigorous mixing and left for 20 min at 0°C . After centrifugation at 10,000 g for 10 min, the supernatants were neutralized with triethanolamine at 0°C. The specific intracellular GSH and GSSG levels were determined as described elsewhere [6, 7, 65].
The intracellular peroxide and superoxide levels were estimated in separate experiments by monitoring the formation of 2',7'-dichlorofluorescein (DCF) from 2',7'-dichlorofluorescin diacetate and ethidium (Et) from dihydroethidium, respectively, as described before [7, 66].
Changes in the specific activities of selected antioxidative enzymes were also measured in separate experiments. In these cases, 18 h mycelia were treated with oxidative stress-inducing agents in minimal-nitrate medium for 1 or 6 h. Stress-exposed mycelia were harvested by filtration on sintered glass, were washed with and re-suspended ad frozen immediately in ice-cold 0.1 M K-phosphate buffer (pH 7.5). Cell-free extracts were prepared by X-pressing and centrifugation . Specific glutathione S-transferase (GST) , catalase  and superoxide dismutase (SOD)  activities were measured according to the literature shown in parentheses.
RNA extraction and Northern blot hybridization
Mycelia from both stress-exposed and control cultures (15 min, 30 min, 1 h, 3 h, 6 h and 9 h cultivation times) were filtered out, washed with ice-cold sterile distilled water, transferred immediately into pre-cooled (-70°C) Eppendorf tubes, were frozen at -70°C and, subsequently, were vacuum-dried overnight at room temperature. Total RNA was extracted from approximately 100 mg quantities of dried mycelial mats using TRISOL reagent (Invitrogen) as recommended by Chomczynski . Crude RNA preparations were dissolved in 300 μl DEPC-treated water by agitation with sterile pipette tips at 68°C for 20–30 min. The samples were cooled down on ice, and RNA pools were precipitated with 300 μl 4.0 M LiCl (prepared in 20 mM Tris-HCl pH = 7.5 buffer, also containing 10 mM EDTA) and were left at -20°C overnight. RNA pellets were collected with centrifugation (12000 g, 30 min, 4°C) and were washed twice with pre-cooled (-20°C) 75 % ethanol. RNA preparations were air-dried and immediately re-suspended in 50 μl aliquots of diethyl pyrocarbonate (DEPC)-treated water. RNA content was always estimated in 300-fold diluted samples by measuring the absorbance at λ = 260 nm. The purity of the preparations was estimated by calculating A260/A280 absorbance ratios, which were always approximately 1.8. RNA preparations were stored at -80°C in 10 μl aliquots to avoid repeated freezing and thawing. The quality of RNA preparations was also checked by running 7.5 μg quantities of RNA in 1.2 % agarose gel.
Electrophoreses and Northern blot analyses were performed as described elsewhere [74–76]. Briefly, 7.5 μg quantities of refined RNA samples were separated in 1.2 % agarose gels containing 3 % formaldehyde and were blotted onto Hybond-N+ (Amersham) membranes. RNA molecular weight calibration was performed using Invitrogen 0.24–9.5 Kb RNA Ladders as standards according to the protocol of the manufacturer. Occasionally, the blots were re-used after stripping them in boiling water in the presence of 0.1 % SDS .
Probes for Northern hybridization were prepared by PCR-amplification of specific sequences for aoxA (mitochondrial alternative oxidase), gstA (glutathione S-transferase), sodA (Cu,Zn-superoxide dismutase precursor), sconC (sulfur metabolic regulator) and ORF AN4483.2 (a close homologue of Schizosaccharomyces pombe Ca2+-calmodulin-dependent serine-threonine-protein kinase) using custom-made cDNA plasmid libraries (Life Technologies) . The oligonucleotide PCR primers employed in this study were: aoxA, F: 5'-TAG CCG ACG AAA CGA TGA CAG GTA-3' and R: 5'-CCT GCT CCG ATG GGG TAT GGA-3'; gstA, F: 5'-GGT AAC GCC TGC TCT TAG GCT A-3' and R: 5'-ATT TCG AGC TCA ACT GAC TGC A-3'; sodA, F: 5'-ATG CGC TCA ACC CTT ACG CAA-3' and R: 5'-CTT TAC CCG CGG CAA GGC TA-3'; sconC, F: 5'-TAA CAA GCA AGA CGA AAC CGC TGA-3' and R: 5'-AAG GGC AAG TCC CCC AGG A-3'; ORF AN4483.2, F: 5'-TTA TGC GCC AGA TCG ATC ACC CTA-3' and R: 5'-GCC AAC ATC AAA GAC TTC ACG CAA-3', all of them designed by the GeneFisher  software. The PCR program included the following steps: 96°C for 3.0 min, 94°C for 0.30 min, t = Tmelting-2.0°C for 0.45 min, 72°C for 1.30 min, amplification cycle repeated 29 times, 72°C for 10.0 min, 15°C for 1 h. To calculate the size of amplified DNA fragments Invitrogen Low DNA Mass Ladder (100–2000 bp) was used in 0.8 % agarose gels prepared in 1 × TAE buffer. Expected and found sizes for cDNA amplificants were always in good agreement.
cDNA fragments were extracted from the 0.8 % agarose gels and purified using QIAEX II Gel Extraction Kit (Qiagen) as recommended by the manufacturer. The DNA preparations were always re-dissolved in ion-exchanged H2O. The efficiency and yield of DNA extraction was always checked by repeated run on 0.8 % agarose gels. 32P-labelled probes were prepared using Invitrogen RadPrime DNA Labelling System according to the manufacturer's protocol employing 25 ng quantities of purified cDNA fragments and 50 μCi (3.3 mM; Amersham Biosciences) 32PdCTP.
Hybridizations were carried out in Church buffer in rolling chambers at 62°C overnight [75, 76, 78]. Membranes were washed once with 2 × SSC/0.2 % SDS and once with 0.1 × SSC/0.1 % SDS solutions at 62°C for 1 h, and were subsequently exposed to Kodak XAR-5 films at -80 C for 2–5 days. Optical densities of autoradiographic bands were recorded with a Model GS-700 Imaging Densitometer (Bio-Rad) and were analyzed with a combined Bio-Rad Gel Doc 1000 and Mitsubishi Video Copy Processor system.
DNA microarray hybridization
The DNA microarrays employed throughout this work contained 3533 unique PCR-amplified probes, identified as first generation in previous publications and printed in 4073 spots, which were duplicated [8, 9]. The full description of gene probes including PCR primer(s), Oklahoma State University contig IDs (OSU contig IDs)  and Broad Institute (Cambridge, MA, USA) ORF IDs  are given at NCBI GEO  on Platforms GPL1752 and GPL1756.
Changes in cDNA populations, prepared from oxidative stress exposed and untreated control cultures, were estimated with Genisphere (Hatfield, PA, USA) 3DNA Submicro EX Expression Array Detection Kit. For each hybridization, 60 μg of total RNA pooled from three biological samples (20 μg each) were used for cDNA synthesis using SuperScript II Reverse Transcriptase Enzyme (Invitrogen). Hybridizations where performed as recommended by Genisphere using the SDS-based hybridization buffer provided with 1.0 μl denaturant COT-1 DNA (10 μg ml-1, GIBCO 15632-011) added, Lifter Slip cover slip (Erie Scientific Co., Portsmouth, NH, USA) and CMT-Hybridization Chamber (Corning Incorporated Life Sciences, Corning, NY, USA) at 65°C. Following hybridization, spot intensities were determined using a GenePix 4000 B microarray scanner (Axon Instruments).
Data normalization and statistics
In DNA microarray experiments, means, medians and standard deviations (S.D.s) for both dyes and for both spot and background intensities were calculated with GenePix Pro 3.0 software. Defected spots with false readings were filtered out manually and were disregarded in further calculations. We also filtered out and omitted those data points where background mean plus1 S.D. was higher than the spot intensity mean for both dyes. After data filtering, the background-corrected ratios and log2 ratios (M) of spot intensities were calculated. Block-by-block LOESS normalization was then applied  using SAS for Windows, version 8 (SAS Institute Inc., Cary, NC, USA.) software package. In further data processing, normalized spot intensity ratios (M') were analyzed.
The effects of different stress-inducing agents on global gene expression patterns were quantified by counting the gene probes responding to the treatments above or below the [+1;-1] M' thresholds ('two-fold rule') . Gene probes affected by more than one agent were also screened. In the latter case, only the first more than two-fold change in gene expression was taken into consideration for each gene probe disregarding all gene probes that responded to different agents oppositely.
Correlation between DNA microarray M' and appropriate M = log2(optical densitystress-exposed*optical densitycontrol-1) values for Northern blot autoradiography pictures was calculated with Microsoft Excel 97 software.
In physiological optimization of experimental conditions, the variations between experiments were estimated by standard deviations (S.D.) and the statistical significance of changes in physiological parameters was estimated by the Student's t-test [6, 7]. Only the probability levels of P ≤ 5 % were regarded as indicative of statistical significance.
Homology search, data mining and significance enrichment calculations
EST sequence processing and functional data sorting were performed using PipeOnline version 2.0 [79, 83]. Homology search was carried out in two independent ways: (i) via translated contig sequence query versus protein in NCBI BLAST (blastx)  and (ii) via translated ORF (Broad Institute, Cambridge, MA, USA)  query versus protein in NCBI BLAST (blastp) . In data sorting, only homologies with Expect value lower than E-40 were considered . Physiological function categories were defined according to previous literature data on transcriptional changes observable under oxidative and other kinds of environmental stress in fungi and PipeOnline functional data sorting based on NCBI protein records and MPW Metabolic Pathways Database functional dictionary [79, 83] (release date December 2002). Importantly, each gene probe was characterized with one annotated function.
For significant enrichment calculations, the hypergeometic distribution test was carried out [86, 87] for selected groups of genes using SAS for Windows, version 8 (SAS Institute Inc., Cary, NC, USA.) software.
List of abbreviations
- CESR –:
core environmental stress response
- DCM –:
dry cell mass
- DCF – 2':
- DEPC –:
- DLM –:
dosis lethalis minima
- EST –:
expressed sequence tag
- Et –:
- FUN –:
- GSH –:
- GSSG –:
- GST –:
- LoaOOH –:
linoleic acid hydroperoxide
- MAPK –:
mitogen-activated protein kinase
- NOX –:
- OSU –:
Oklahoma State University
- ROS –:
reactive oxygen species
- SAPK –:
stress-activated protein kinase
- SOD –:
The authors are indebted to Dr. Manda E. Gent (COGEME, University of Manchester) for printing the DNA microarrays used in this study. Dr. László Nagy and Dr. Beáta Scholtz (University of Debrecen) provided the Authors with an excellent technical background to perform DNA microarray hybridizations in Debrecen. The authors are grateful to Dr. Pál Gergely, vice president of the Medical and Health Science Center, University of Debrecen, for his encouragement and support to start Aspergillus genomic research in Debrecen. One of us (I.P.) was a recipient of a Fulbright Research Fellowship to the Oklahoma State University and a Széchenyi István Scholarship. E.T. was a grantee of the Bolyai János Scholarship. This project was supported financially by the Hungarian Office for Higher Education Programmes (grant reference number 0092/2001) and the Hungarian Scientific Research Fund (grant reference number T037473).
- Schafer FQ, Buettner GR: Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Radic Biol Med. 2001, 30: 1191-1212. 10.1016/S0891-5849(01)00480-4.PubMedView ArticleGoogle Scholar
- Finkel T: Oxidant signals and oxidative stress. Curr Opin Cell Biol. 2003, 15: 247-254. 10.1016/S0955-0674(03)00002-4.PubMedView ArticleGoogle Scholar
- Aguirre J, Ríos-Momberg M, Hewitt D, Hansberg W: Reactive oxygen species and development in microbial eukaryotes. Trends Microbiol. 2005, 13: 111-118. 10.1016/j.tim.2005.01.007.PubMedView ArticleGoogle Scholar
- Lambeth JD: NOX enzymes and the biology of reactive oxygen. Nat Rev Immunol. 2004, 4: 181-189. 10.1038/nri1312.PubMedView ArticleGoogle Scholar
- Thorpe GW, Fong CS, Alic N, Higgins VJ, Dawes IW: Cells have distinct mechanisms to maintain protection against different reactive oxygen species: Oxidative-stress-response genes. Proc Natl Acad Sci USA. 2004, 101: 6564-6569. 10.1073/pnas.0305888101.PubMedPubMed CentralView ArticleGoogle Scholar
- Emri T, Pócsi I, Szentirmai A: Glutathione metabolism and protection against oxidative stress caused by peroxides in Penicillium chrysogenum. Free Radic Biol Med. 1997, 23: 809-814. 10.1016/S0891-5849(97)00065-8.PubMedView ArticleGoogle Scholar
- Emri T, Pócsi I, Szentirmai A: Analysis of the oxidative stress response of Penicillium chrysogenum to menadione. Free Radic Res. 1999, 30: 125-132.PubMedView ArticleGoogle Scholar
- Sims AH, Robson GD, Hoyle DC, Oliver SG, Turner G, Prade RA, Russell HH, Dunn-Coleman NS, Gent ME: Use of expressed sequence tag analysis and cDNA microarrays of the filamentous fungus Aspergillus nidulans. Fungal Genet Biol. 2004, 41: 199-212. 10.1016/j.fgb.2003.11.005.PubMedView ArticleGoogle Scholar
- Sims AH, Gent ME, Lanthaler K, Dunn-Coleman NS, Oliver SG, Robson GD: Transcriptome analysis of recombinant protein secretion by Aspergillus nidulans and the unfolded-protein response in vivo. Appl Environ Microbiol. 2005, 71: 2737-2747. 10.1128/AEM.71.5.2737-2747.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Ray A, Macwana S, Ayoubi P, Hall LT, Prade R, Mort AJ: Negative substraction hybridisation: an efficient method to isolate large numbers of condition-specific cDNAs. BCM Genomics. 2004, 5 (22): [http://www.biomedcentral.com/1471-2164/5/22]Google Scholar
- Emri T, Molnár Z, Pusztahelyi T, Rosén S, Pócsi I: Effect of vitamin E on the autolysis and sporulation of Aspergillus nidulans. Appl Biochem Biotechnol. 2004, 118: 337-348. 10.1385/ABAB:118:1-3:337.PubMedView ArticleGoogle Scholar
- Molnár Z, Mészáros E, Szilágyi Z, Rosén S, Emri T, Pócsi I: Influence of fadA G203R and ΔflbA mutations on the morphology and physiology of submerged Aspergillus nidulans cultures. Appl Biochem Biotechnol. 2004, 118: 349-360. 10.1385/ABAB:118:1-3:349.PubMedView ArticleGoogle Scholar
- National Center for Biotechnology Information Gene Expression Omnibus. [http://www.ncbi.nlm.nih.gov/geo/]
- Halliwell B, Gutteridge JMC: Free Radicals in Biology and Medicine. 1999, Oxford: Oxford Science PublicationsGoogle Scholar
- Pócsi I, Prade RA, Penninckx MJ: Glutathione, altruistic metabolite in fungi. Adv Microb Physiol. 2004, 49: 1-76. 10.1016/S0065-2911(04)49001-8.PubMedView ArticleGoogle Scholar
- Fortuniak A, Żądziński R, Biliński T, Bartosz G: Glutathione depletion in the yeast Saccharomyces cerevisiae. Biochem Mol Biol Int. 1996, 38: 901-910.PubMedGoogle Scholar
- Kosower NS: The glutathione status of the cells. Int Rev Cytol. 1978, 54: 109-160.PubMedView ArticleGoogle Scholar
- Toledano MB, Delaunay A, Biteau B, Spector D, Azevedo D: Oxidative stress responses in yeast. Yeast Stress Responses. Edited by: Hohman S, Mager WH. 2003, Berlin: Springer-Verlag, 305-387.Google Scholar
- Flattery-O'Brien J, Collinson LP, Dawes IW: Saccharomyces cerevisiae has an inducible response to menadione which differs from that to hydrogen-peroxide. J Gen Microbiol. 1993, 139: 501-507.PubMedView ArticleGoogle Scholar
- Jamieson DJ, Rivers SL, Stephen DWS: Analysis of Saccharomyces cerevisiae proteins induced by peroxide and superoxide stress. Microbiology. 1994, 140: 3277-3283.PubMedView ArticleGoogle Scholar
- Lee J, Dawes IW, Roe JH: Adaptive response of Schizosaccharomyces pombe to hydrogen peroxide and menadione. Microbiology. 1995, 141: 3127-3132.PubMedView ArticleGoogle Scholar
- Stephen DWS, Rivers SL, Jamieson DJ: The role of the Yap1 and Yap2 genes in the regulation of the adaptive oxidative stress responses of Saccharomyces cerevisiae. Mol Microbiol. 1995, 16: 415-423.PubMedView ArticleGoogle Scholar
- Buisson N, Labbe-Bois R: Flavohemoglobin expression and function in Saccharomyces cerevisiae – No relationship with respiration and complex response to oxidative stress. J Biol Chem. 1998, 273: 9527-9533. 10.1074/jbc.273.16.9527.PubMedView ArticleGoogle Scholar
- Gasch AP, Spellman PT, Kao CM, Carmel-Harel O, Eisen MB, Storz G, Botstein D, Brown PO: Genomic expression programs in the response of yeast cells to environmental changes. Mol Biol Cell. 2000, 11: 4241-4257.PubMedPubMed CentralView ArticleGoogle Scholar
- Osorio H, Carvalho E, del Valle M, Sillero MAG, Moradas-Ferreira P, Sillero A: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae – An experimental and theoretical approach. Eur J Biochem. 2003, 270: 1578-1589. 10.1046/j.1432-1033.2003.03529.x.PubMedView ArticleGoogle Scholar
- Chae K, Lee K: Sensitivity of a hyperactivated Ras mutant in response to hydrogen peroxide, menadione and paraquat. Bull Kor Chem Soc. 1998, 19: 1202-1206.Google Scholar
- Lee J, Kwon ES, Kim DW, Cha JS, Roe JH: Regulation and the role of Cu,Zn-containing superoxide dismutase in cell cycle progression of Schizosaccharomyces pombe. Biochem Biophys Res Commun. 2002, 297: 854-862. 10.1016/S0006-291X(02)02290-8.PubMedView ArticleGoogle Scholar
- Azevedo D, Tacnet F, Delaunay A, Rodrigues-Pousada C, Toledano MB: Two redox centers within Yap1 for H2O2 and thiol-reactive chemicals signaling. Free Radic Biol Med. 2003, 35: 889-900. 10.1016/S0891-5849(03)00434-9.PubMedView ArticleGoogle Scholar
- Evans MV, Turton HE, Grant CM, Dawes IW: Toxicity of linoleic acid hydroperoxide to Saccharomyces cerevisiae : involvement of a respiration-related process for maximal sensitivity and adaptive response. J Bacteriol. 1998, 180: 483-490.PubMedPubMed CentralGoogle Scholar
- Shertzer HG, Lastbom L, Sainsbury M, Moldeus P: Menadione-mediated membrane fluidity alterations and oxidative damage in rat hepatocytes. Biochem Pharmacol. 1992, 43: 2135-2141. 10.1016/0006-2952(92)90172-F.PubMedView ArticleGoogle Scholar
- Jayashree T, Subramanyam C: Oxidative stress as a prerequisite for aflatoxin production by Aspergillus parasiticus. Free Radic Biol Med. 2000, 29: 981-985. 10.1016/S0891-5849(00)00398-1.PubMedView ArticleGoogle Scholar
- Alic N, Felder T, Temple MD, Gloeckner C, Higgins VJ, Briza P, Dawes IW: Genome-wide transcriptional responses to a lipid hydroperoxide: adaptation occurs without induction of oxidant defenses. Free Radic Biol Med. 2004, 37: 23-35. 10.1016/j.freeradbiomed.2004.04.014.PubMedView ArticleGoogle Scholar
- Saito H, Tatebayashi K: Regulation of the osmoregulatory HOG MAPK cascade in yeast. J Biochem (Tokyo). 2004, 136: 267-272.View ArticleGoogle Scholar
- Rutter J, Probst BL, McKnight SL: Coordinate regulation of sugar flux and translation by PAS kinase. Cell. 2002, 11: 17-28. 10.1016/S0092-8674(02)00974-1.View ArticleGoogle Scholar
- Yamada K, Nakagawa CW, Mutoh N: Schizosaccharomyces pombe homologue of glutathione peroxidase, which does not contain selenocysteine, is induced by several stresses and works as an antioxidant. Yeast. 1999, 15: 1125-1132. 10.1002/(SICI)1097-0061(199908)15:11<1125::AID-YEA442>3.0.CO;2-Z.PubMedView ArticleGoogle Scholar
- Paredes V, Franco A, Soto T, Vicente-Soler J, Gacto M, Cansado J: Different roles for the stress-activated protein kinase pathway in the regulation of trehalose metabolism in Schizosaccharomyces pombe. Microbiology. 2003, 149: 1745-1752. 10.1099/mic.0.26279-0.PubMedView ArticleGoogle Scholar
- Chen D, Toone WM, Mata J, Lyne R, Burns G, Kivinen K, Brazma A, Jones N, Bähler J: Global transcriptional responses of fission yeast to environmental stress. Mol Biol Cell. 2003, 14: 214-229. 10.1091/mbc.E02-08-0499.PubMedPubMed CentralView ArticleGoogle Scholar
- Nishikawa T, Aiba H, Mizuno T: The cta3+ gene that encodes a cation-transporting P-type ATPase is induced by salt stress under control of the Wis1-Sty1 MAPKK-MAPK cascade in fission yeast. FEBS Lett. 1999, 455: 183-187. 10.1016/S0014-5793(99)00876-5.PubMedView ArticleGoogle Scholar
- Rigobello MP, Galzigna L, Bindoli A: Fructose 1,6-bisphosphate prevents oxidative stress in the isolated and perfused rat heart. Cell Biochem Funct. 1994, 12: 69-75. 10.1002/cbf.290120110.PubMedView ArticleGoogle Scholar
- Lian XY, Stringer JL: Energy failure in astrocytes increases the vulnerability of neurons to spreading depression. Eur J Neurosci. 2004, 19: 2446-2454. 10.1111/j.0953-816X.2004.03289.x.PubMedView ArticleGoogle Scholar
- He X, Hayashi N, Walcott NG, Azuma Y, Patterson TE, Bischoff FR, Nishimoto T, Sazer S: The identification of cDNAs that affect the mitosis-to-interphase transition in Schizosaccharomyces pombe, including sbp1, which encodes a spi1p-GTP-binding protein. Genetics. 1998, 148: 645-656.PubMedPubMed CentralGoogle Scholar
- Wong DH, Corbett AH, Kent HM, Stewart M, Silver PA: Interaction between the small GTPase Ran/Gsp1p and Ntf2p is required for nuclear transport. Mol Cell Biol. 1997, 17: 3755-3767.PubMedPubMed CentralView ArticleGoogle Scholar
- Corbett AH, Silver PA: The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo. J Biol Chem. 1996, 271: 18477-18484. 10.1074/jbc.271.37.22672.PubMedView ArticleGoogle Scholar
- Nakashima N, Noguchi E, Nishimoto T: Saccharomyces cerevisiae putative G protein, Gtr1p, which forms complexes with itself and a novel protein designated as Gtr2p, negatively regulates the Ran/Gsp1p G protein cycle through Gtr2p. Genetics. 1999, 152: 853-867.PubMedPubMed CentralGoogle Scholar
- Kim MJ, Choi JW, Park SM, Cha BJ, Yang MS, Kim DH: Characterization of a fungal protein kinase from Cryphonectria parasitica and its transcriptional upregulation by hypovirus. Mol Microbiol. 2002, 45: 933-941. 10.1046/j.1365-2958.2002.03079.x.PubMedView ArticleGoogle Scholar
- Busch S, Bode HB, Brakhage AA, Braus GH: Impact of the cross-pathway control on the regulation of lysine and penicillin biosynthesis in Aspergillus nidulans. Curr Genet. 2003, 42: 209-219.PubMedGoogle Scholar
- Strittmatter AW, Irniger S, Braus GH: Induction of jlbA mRNA synthesis for a putative bZIP protein of Aspergillus nidulans by amino acid starvation. Curr Genet. 2001, 39: 327-334. 10.1007/s002940100215.PubMedView ArticleGoogle Scholar
- Barros TF, Puccia R: Cloning and characterization of a LON gene homologue from the human pathogen Paracoccidioides brasiliensis. Yeast. 2001, 18: 981-988. 10.1002/yea.745.PubMedView ArticleGoogle Scholar
- Honigberg SM: Ime2p and Cdc28p: co-pilots driving meiotic development. J Cell Biochem. 2004, 92: 1025-1033. 10.1002/jcb.20131.PubMedView ArticleGoogle Scholar
- Purnapatre K, Gray M, Piccirillo S, Honigberg SM: Glucose inhibits meiotic DNA replication through SCFGrr1p-dependent destruction of Ime2p kinase. Mol Cell Biol. 2005, 25: 440-450. 10.1128/MCB.25.1.440-450.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Stephens KE, Miller KY, Miller BL: Functional analysis of DNA sequences required for conidium-specific expression of the SpoC1-C1C gene of Aspergillus nidulans. Fungal Genet Biol. 1999, 27: 231-242. 10.1006/fgbi.1999.1145.PubMedView ArticleGoogle Scholar
- Ohi R, McCollum D, Hirani B, Den Haese GJ, Zhang X, Burke JD, Turner K, Gould KL: The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb. EMBO J. 1994, 13: 471-483.PubMedPubMed CentralGoogle Scholar
- Miyazaki Y, Jojima T, Ono T, Yamazaki T, Shishido K: A cDNA homologue of Schizosaccharomyces pombe cdc5+ from the mushroom Lentinula edodes: characterization of the cDNA and its expressed product. Biochim Biophys Acta. 2004, 1680: 93-102.PubMedView ArticleGoogle Scholar
- Jung MK, May GS, Oakley BR: Mitosis in wild-type and β-tubulin mutant strains of Aspergillus nidulans. Fungal Genet Biol. 1998, 24: 146-160. 10.1006/fgbi.1998.1057.PubMedView ArticleGoogle Scholar
- Flattery-O'Brien JA, Dawes IW: Hydrogen peroxide causes RAD9-dependent cell cycle arrest in G2 in Saccharomyces cerevisiae whereas menadione causes G1 arrest independent of RAD9 function. J Biol Chem. 1998, 273: 8564-8571. 10.1074/jbc.273.15.8564.PubMedView ArticleGoogle Scholar
- Forman HJ, Torres M: Reactive oxygen species and cell signaling: respiratory burst in macrophage signaling. Am J Respir Crit Care Med. 2002, 166: S4-S8. 10.1164/rccm.2206007.PubMedView ArticleGoogle Scholar
- Alblas J, Honing H, Renardel de Lavalette C, Brown MH, Dijkstra CD, van den Berg TK: Signal regulatory protein α ligation induces macrophage nitric oxide production through JAK/STAT- and phosphatidylinositol 3-kinase/Rac1/NAPDH oxidase/H2O2-dependent pathways. Mol Cell Biol. 2005, 25: 7181-7192. 10.1128/MCB.25.16.7181-7192.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Goldstein BJ, Mahadev K, Wu X, Zhu L, Motoshima H: Role of insulin-induced reactive oxygen species in the insulin signaling pathway. Antioxid Redox Signal. 2005, 7: 1021-1031. 10.1089/ars.2005.7.1021.PubMedPubMed CentralView ArticleGoogle Scholar
- Lassègue B, Sorescu D, Szőcs K, Yin Q, Akers M, Zhang Y, Grant SL, Lambeth JD, Griendling KK: Novel gp91phox homologues in vascular smooth muscle cells : nox1 mediates angiotensin II-induced superoxide formation and redox-sensitive signaling pathways. Circ Res. 2001, 88: 888-894.PubMedView ArticleGoogle Scholar
- Foreman J, Demidchik V, Bothwell JH, Mylona P, Miedema H, Torres MA, Linstead P, Costa S, Brownlee C, Jones JD, Davies JM, Dolan L: Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. Nature. 2003, 422: 442-446. 10.1038/nature01485.PubMedView ArticleGoogle Scholar
- Lara-Ortiz T, Riveros-Rosas H, Aguirre J: Reactive oxygen species generated by microbial NADPH oxidase NoxA regulate sexual development in Aspergillus nidulans. Mol Microbiol. 2003, 50: 1241-1255. 10.1046/j.1365-2958.2003.03800.x.PubMedView ArticleGoogle Scholar
- Malagnac F, Lalucque H, Lepere G, Silar P: Two NADPH oxidase isoforms are required for sexual reproduction and ascospore germination in the filamentous fungus Podospora anserina. Fungal Genet Biol. 2004, 41: 982-997. 10.1016/j.fgb.2004.07.008.PubMedView ArticleGoogle Scholar
- Castrillo JI, Oliver SG: Yeast as a touchstone in post-genomic research: strategies for integrative analysis in functional genomics. J Biochem Mol Biol. 2004, 37: 93-106.PubMedView ArticleGoogle Scholar
- Barratt RW, Johnson GB, Ogata WN: Wild-type and mutant stocks of Aspergillus nidulans. Genetics. 1965, 52: 233-246.PubMedPubMed CentralGoogle Scholar
- Anderson ME: Determination of glutathione and glutathione disulfide in biological samples. Method Enzymol. 1985, 113: 548-555.View ArticleGoogle Scholar
- Sámi L, Emri T, Pócsi I: Autolysis and ageing of Penicillium chrysogenum cultures under carbon starvation: glutathione metabolism and formation of reactive oxygen species. Mycol Res. 2001, 105: 1246-1250.View ArticleGoogle Scholar
- Leiter É, Emri T, Gyémant G, Nagy I, Pócsi I, Winkelmann G, Pócsi I: Penicillin V production by Penicillium chrysogenum in the presence of Fe(III) and in low-iron culture medium. Folia Microbiol. 2001, 46: 183-186.View ArticleGoogle Scholar
- Warholm M, Guthenberg C, von Bahr C, Mannervik B: Glutathione transferases from human liver. Methods Enzymol. 1985, 113: 499-504.PubMedView ArticleGoogle Scholar
- Roggenkamp R, Sahm H, Wagner F: Microbial assimilation of methanol induction and function of catalase in Candida boidinii. FEBS Lett. 1974, 41: 283-286. 10.1016/0014-5793(74)81230-5.PubMedView ArticleGoogle Scholar
- Oberley LW, Spitz DR: Assay of superoxide dismutase activity in tumour tissue. Methods Enzymol. 1984, 105: 457-464.PubMedView ArticleGoogle Scholar
- Pusztahelyi T, Pócsi I, Kozma J, Szentirmai A: Aging of Penicillium chrysogenum cultures under carbon starvation: I: morphological changes and secondary metabolite production. Biotechnol Appl Biochem. 1997, 25: 81-86.View ArticleGoogle Scholar
- Peterson GL: Determination of total protein. Methods Enzymol. 1983, 91: 86-105.Google Scholar
- Chomczynski P: A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques. 1993, 15: 532-536.PubMedGoogle Scholar
- Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. 1989, Cold Spring Harbor: Cold Spring Harbor Laboratory, 2Google Scholar
- Yu JH, Butchko RA, Fernandes M, Keller NP, Leonard TJ, Adams TH: Conservation of structure and function off the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. flavus. Curr Genet. 1996, 29: 549-555. 10.1007/s002940050084.PubMedView ArticleGoogle Scholar
- Han KH, Prade RA: Osmotic stress-coupled maintenance of growth in Aspergillus nidulans. Mol Microbiol. 2002, 43: 1065-1078. 10.1046/j.1365-2958.2002.02774.x.PubMedView ArticleGoogle Scholar
- GeneFisher Interactive PCR Primer Design. [http://bibiserv.techfak.uni-bielefeld.de/genefisher/]
- Church GM, Gilbert W: Genomic sequencing. Proc Natl Acad Sci USA. 1984, 81: 1991-1995.PubMedPubMed CentralView ArticleGoogle Scholar
- PipeOnline. An online resource for data mining of processed DNA sequence databases. [http://bioinfo.okstate.edu/pipeonline/]
- Broad Institute Aspergillus nidulans Database. [http://www.broad.mit.edu/annotation/fungi/aspergillus/]
- Leung YF, Cavalieri D: Fundamentals of cDNA microarray data analysis. Trends Genet. 2003, 19: 649-659. 10.1016/j.tig.2003.09.015.PubMedView ArticleGoogle Scholar
- Schena M, Shalon D, Heller R, Chai A, Brown PO, Davis RW: Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes. Proc Natl Acad Sci USA. 1996, 93: 10614-10619. 10.1073/pnas.93.20.10614.PubMedPubMed CentralView ArticleGoogle Scholar
- Ayoubi P, Jin X, Leite S, Liu X, Martajaja J, Abduraham A, Wan Q, Yan W, Misawa E, Prade RA: PipeOnline 2.0: automated EST processing and functional data sorting. Nucleic Acids Res. 2002, 30: 4761-4769. 10.1093/nar/gkf585.PubMedPubMed CentralView ArticleGoogle Scholar
- NCBI Basic Local Alignment Search Tool (BLAST). [http://www.ncbi.nlm.nih.gov/BLAST/]
- Andrade MA, Brown NP, Leroy C, Hoersch S, de Daruvar A, Reich C, Franchini A, Tamames J, Valencia A, Ouzounis C, Sander C: Automated genome sequence analysis and annotation. Bioinformatics. 1999, 15: 391-412. 10.1093/bioinformatics/15.5.391.PubMedView ArticleGoogle Scholar
- Hsiao A, Ideker T, Olefsky JM, Subramaniam S: VAMPIRE microarray suite: a web-based platform for the interpretation of gene expression data. Nucleic Acids Res. 2005, 33: W627-W632. 10.1093/nar/gki443.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang B, Schmoyer D, Kirov S, Snoddy J: GOTree Machine (GOTM): a web-based platform for interpreting sets of interesting genes using Gene Ontology hierarchies. BMC Bioinformatics. 2005, 6 (72): [http://www.biomedcentral.com/1471-2105/5/16]Google Scholar
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