Table 4 Primer strategies for alternate versions of the study protocol.
From: A robust method for the amplification of RNA in the sense orientation
Type of Total RNA | First Primer | Second Primer | IVT System† | Type of Product | Orientation of Product |
|---|---|---|---|---|---|
Eukaryotic | Oligo(dT)24, T7-Oligo(dT)24 | T3N9 | T3 | mRNA | Sense |
T3-Oligo(dT)24 | Random Hexamer | T3 | mRNA | Antisense* | |
Prokaryotic (or Partially Degraded**) | Random Hexamer | T3N9 | T3 | Total RNA | Sense |
T3N9 | Random Hexamer | T3 | Total RNA | Antisense |