Figure 4

Multiple alignment of the GBD/FH3 domains of Dictyostelium formins and two RasGEFs Amino acid sequences were aligned with ClustalX and the output file was subsequently edited manually. In addition to nine Dictyostelium formins, a GBD/FH3 domain was identified also at the N-terminus of RasGEF-L and RasGEF-V. The sequence of the human DRF3 has been included for reference. Dashes indicate gaps introduced for optimal alignment. In some places extensive repetitive stretches have been removed and replaced by a figure indicating the number of residues omitted. Residues identical or similar in at least 40% of the sequences are boxed in black or gray, respectively. Continuous and discontinuous lines indicate, respectively, the extension of the GBD and FH3 domains as defined in the Pfam database. Short arrows indicate boundaries of the FH3 domain as proposed by Petersen et al. [14]. Conserved intron positions are labeled a and b (see Fig. 1).