Figure 5

Phylogenetic tree of the GBD/FH3 domains of formins and two RasGEFs from Dictyostelium and formins from other organisms. Amino acid sequences of the GBD/FH3 domains were aligned with ClustalX and the output file was subsequently edited manually and intervening sequences between blocks of high similarity were removed. The sequence available for DmAE003560 only contains a FH3 domain and a short part of the GBD. A bootstrapped unrooted phylogenetic tree was constructed as described in the Methods section. Dictyostelium members are indicated in red. The other organisms considered are as in the legend to figure 3. Labeling is also as in the legend to figure 3.