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Table 1 Parameters of constructed SAGE libraries from B6 mouse total testis

From: Global transcriptome analysis of the C57BL/6J mouse testis by SAGE: evidence for nonrandom gene order

SAGE library

Total testis 1 (TT 1)

Total testis 2 (TT 2)

Sequenced clones

811

1 510

Total tags*

24 975

51 879

Unique transcripts

10 516

18 848

Single copy tags

7 731

13 422

Quality parameters

  

Average tags per clone

30.8

34.4

Duplicated ditags

157 (1.2 %)

276 (1.0 %)

Linker derived tags

147 (0.6 %)

223 (0.4 %)

  1. * excluding tags from duplicated ditags and linker-derived tags (i.e. two linker tags TCCCTATTAA, TCCCCGTACA and all possible 1-bp linker tag variations)
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BMC Genomics

ISSN: 1471-2164

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