Figure 4

Comparison of microarray and qRT-PCR gene expression profiles for selected genes. X-Y plots were generated from the ratio of transcript levels in the blood-fed adult female mosquitoes to the transcript levels in the sugar-fed adult female mosquitoes for eight selected genes at five time points including 0, 5, 12, 24 and 48 hours post blood meal (PBM). Genes were randomly selected from the three sets and included ASs 648 and 1786 from the Early Genes (A), ASs 996, 1158, 1949, and 679 from the Middle Genes (B), and ASs 12 and 1357 from the Late Genes (C). Triangles indicate fold expression data generated by qRT-PCR analysis; circles indicate fold expression data generated by microarray analysis. A horizontal line connecting either the diamonds or circles illustrates each gene expression profile. For each gene, the expression profiles created by qRT-PCR and microarray analysis are indicated using the same color.