A highly redundant BAC library of Atlantic salmon (Salmo salar): an important tool for salmon projects

BMC Genomics

Table 3 Details of the three segments of the BAC library

Segment	1	2	3	All segments
Plate numbers	1–288	289–576	577–816	1–816
Plate count	288	288	240	816
Empty wells	1982 (1.79%)	2830 (2.56%)	2975 (3.23%)	7787 (2.49%)
Non-recombinant clones	0	5	4	9
Non-insert clones ¹	1.2% ²	2.8% ³	2.3% ⁴	2.0%
Recombinant clones ⁵	~107 000	~105 000	~87 000	~299 000
Average insert size	189 Kbp	190 Kbp	186 Kbp	188 Kbp
Genomic coverage	6.8X	6.6X	5.4X	18.8X

Genomic DNA obtained from Salmon sperm was partially digested with EcoRI restriction enzyme and cloned in pTARBAC2.1 vector.
¹ Estimated numbers for each segment is based upon NotI digestion.
² 3 out of 249 clones were identified as non-insert clones
³ 6 out of 218 clones were identified as non-insert clones
⁴ 5 out of 220 clones were identified as non-insert clones
⁵ Recombinant clones have been estimated from the total well number subtracting non-insert clones, non-recombinant clones and empty wells.

ISSN: 1471-2164