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Table 2 A comparison of average qRT-PCR, RMA, and MAS 5.0 scores and the corresponding correlation values for the 17 genes assayed in this study for which the Affymetrix microarray probesets (Affy IDs) may not recognize the exact same transcript subsets recognized by qRT-PCR probes. Genes are ranked from lowest to highest average log2 RMA scores. Genes with significant correlations (p < 0.05) obtained by either normalization procedure are highlighted in bold. The number of specimens tested for each gene is included (n). Expression levels are shown as log2.

From: Gene expression levels assessed by oligonucleotide microarray analysis and quantitative real-time RT-PCR – how well do they correlate?

GENE

 

EXPRESSION

CORRELATION

NAME

AFFY ID

n

RMA

MAS 5.0

qRT-PCR

RMA-qRT-PCR

MAS-qRT-PCR

CDC14A

210742_at

13

5.77

7.64

-0.67

0.31

0.26

P125

209175_at

19

6.61

8.43

0.54

0.11

-0.11

GCLC

202922_at

13

6.65

9.20

0.33

0.46

0.56

MAP3K7

206853_s_at

13

6.65

8.72

1.19

0.11

-0.10

TIAL1

202405_at

19

6.68

7.86

0.30

0.32

0.17

FLJ20003

219067_s_at

19

6.71

8.54

0.64

0.64

0.34

RUNX1

210365_at

13

6.95

9.24

1.47

0.29

0.28

PLEKHA1

219024_at

19

6.99

8.18

-2.88

-0.40

-0.28

FLJ12661

218420_s_at

19

7.35

8.50

0.57

-0.08

-0.17

RGC32

218723_s_at

19

7.36

8.11

-3.20

0.85

0.96

WDR11

218090_s_at

19

7.96

9.04

0.60

0.12

0.01

RFC3

204127_at

19

8.10

9.77

1.20

0.62

0.64

ASAH1

213702_x_at

22

8.30

10.29

1.46

0.29

0.27

P38IP

220408_x_at

19

8.35

9.55

0.76

0.73

0.65

BUB3

201456_s_at

19

8.41

9.70

0.60

0.64

0.61

SAC2

203607_at

19

8.86

10.21

0.35

0.22

0.12

TSC22

215111_s_at

19

10.56

11.87

-1.34

0.83

0.82