Changes in the transcriptional profile of transporters in the intestine along the anterior-posterior and crypt-villus axes

Table 5 Segment and tissue specificity of transporters along the A-P and crypt-villus axis.

Transporter	Segment-Specificity^a,M	Segment-Specificity^a,R	Tissue-Specificity^b
			Duodenum	Jejunum	Ileum	Colon
Abca1	D=J=I=C	J=I=C>D	Crypts>Villi	ND	ND	ND
Abcc1	D=J=I=C	I=C>D=J	Crypts>Villi	Crypts>Villi	Crypts>Villi	ND
Abcc3	D=C>J=C>I=C	I=C>D=J	ND	Crypts>Villi	ND	Crypts>Villi
Abcg8	J>I=D>C	D=J=I>C	ND	Villi>Crypts	Villi>Crypts	ND
Slc10a2	I>C>D=J	I>C>D=J	ND	ND	Villi>Crypts	ND
Slc28a2	D>J>I>C	D=J=I>C	ND	Villi>Crypts^x	Villi>Crypts^x	ND
Slc2a1	C>D=J=I	C>I>D=J	ND	Crypts>Villi	Crypts>Villi	Crypts>Villi^x
Slc34a2	I>C>J>D	I>C=J>D	ND	Villi>Crypts	Villi>Crypts	Villi>Crypts
Slc5a8	C>I>D=J	C=I>J>D	ND	Villi>Crypts	ND	ND

^aExpression in the four intestinal segments (D, J, I, C) was measured in whole tissue using microarrays (M) and RT-PCR (R) (n = 3, p ≤ 0.05). ^bTissue specificity was determined in laser dissected crypts and villi (n = 5, where p ≤ 0.05 or if indicated with a superscript "*" p ≤ 0.10). "ND" indicates that no significant difference in expression levels were measured between the crypt and villus epithelial cells.

ISSN: 1471-2164