Pigs in sequence space: A 0.66X coverage pig genome survey based on shotgun sequencing
- Rasmus Wernersson†1,
- Mikkel H Schierup†2,
- Frank G Jørgensen2,
- Jan Gorodkin3,
- Frank Panitz4,
- Hans-Henrik Stærfeldt1,
- Ole F Christensen2,
- Thomas Mailund2,
- Henrik Hornshøj4,
- Ami Klein3,
- Jun Wang5, 6,
- Bin Liu6,
- Songnian Hu6,
- Wei Dong6,
- Wei Li6,
- Gane KS Wong6,
- Jun Yu6,
- Jian Wang6,
- Christian Bendixen4,
- Merete Fredholm3,
- Søren Brunak1,
- Huanming Yang6Email author and
- Lars Bolund5, 6Email author
© Wernersson et al; licensee BioMed Central Ltd. 2005
Received: 13 December 2004
Accepted: 10 May 2005
Published: 10 May 2005
Comparative whole genome analysis of Mammalia can benefit from the addition of more species. The pig is an obvious choice due to its economic and medical importance as well as its evolutionary position in the artiodactyls.
We have generated ~3.84 million shotgun sequences (0.66X coverage) from the pig genome. The data are hereby released (NCBI Trace repository with center name "SDJVP", and project name "Sino-Danish Pig Genome Project") together with an initial evolutionary analysis.
The non-repetitive fraction of the sequences was aligned to the UCSC human-mouse alignment and the resulting three-species alignments were annotated using the human genome annotation. Ultra-conserved elements and miRNAs were identified. The results show that for each of these types of orthologous data, pig is much closer to human than mouse is. Purifying selection has been more efficient in pig compared to human, but not as efficient as in mouse, and pig seems to have an isochore structure most similar to the structure in human.
The addition of the pig to the set of species sequenced at low coverage adds to the understanding of selective pressures that have acted on the human genome by bisecting the evolutionary branch between human and mouse with the mouse branch being approximately 3 times as long as the human branch. Additionally, the joint alignment of the shot-gun sequences to the human-mouse alignment offers the investigator a rapid way to defining specific regions for analysis and resequencing.
The domesticated pig (Sus scrofa) is an obvious choice for genome sequencing, because of its important economic value for meat production and its relevance to biomedical research. The evolutionary position of the pig as an artiodactyl, where no other large scale sequencing efforts have so far been published, makes it valuable for comparative genomics.
Comparative vertebrate genome analysis – e.g. with the aim of understanding evolutionary pressures on the human sequence – is most cost-effective with relatively low coverage, genome wide sequencing of species at different evolutionary distances [1, 2]. The mouse and rat genomes [3, 4] and the chimpanzee genome  have offered sequences, which are evolutionary quite distant and very close to the human genome sequence, respectively. The evolutionary close chimpanzee sequence can pinpoint significant recent changes in genes, but is not efficient for identification of important regions by comparative approaches because many regions are extremely conserved by chance effects and differences in mutation rates over the genome. The more distantly related rodent sequences are useful in the search for conserved regions of biological importance. However, more species are needed, and an artiodactyl like pig is an obvious choice for the following reasons. 1), By comparing rodents and human, it is not possible to determine whether observable differences, e.g. difference in isochore structure, are mainly due to changes in the rodent or primate lineage since their divergence (approximately 90 million years ago, see 6); 2) Rodents have only a subset of the biological functions important to humans. By including pig additional functions will be covered; 3) Even though rodents, artiodactyls and primates diverged at approximately the same time [6, 7], molecular evolution has been faster in the rodent branch, thus the pig is expected to be closer in sequence to human than mouse is. The importance of these points is generally appreciated and comparative genomics sequencing initiatives focusing on restricted regions of the genomes have recently provided much insight [e.g. [1, 2]].
The Chinese-Danish pig sequencing consortium has generated about 3.84 million high quality sequences from 5 pig breeds. The present study releases these data and reports an initial evolutionary analysis which confirms that pig and human are closer in sequence space and quantifies the rates of evolution in the pig, rodent and human lineages for various categories of the genome sequences.
Overview of the number of raw reads generated from each breed.
Number of high quality reads
Number of bases
By aligning the set of ultra-conserved regions against the pig genome reads using BLAST, we were able to find 239 of the 481 known regions reported in Bejerano et al. (2004) with a significant hit of at least 150 bp. Only 12 of these regions were less than 98% conserved (85–97% identity). This result agrees very well with the expected 48% of the pig genome being covered and the assumption that these regions are very well conserved within Mammalia.
By aligning the pig shotgun data against all human transcripts (NCBI build 34) we found 758 completely conserved sequences exceeding 200 bp in length. Of these, 41 were also found to be completely conserved in the mouse genome, while 590 were less conserved (more than 95% identity over at least 80% of the length). BLASTing human transcripts vs. the fully assembled mouse genome (NCBI build 32), we found 2709 ultra-conserved regions. When aligning this set of sequences against the artificially fragmented mouse genomic dataset using BLAST it was only possible to classify 664 (24.5%) as ultra-conserved – less than the 758 elements found in the human-pig comparison.
The set of pig miRNAs (Additional file 2) was compared to human and mouse and it was possible to obtain 50 three-way alignments. The evolutionary tree in Figure 1 was constructed using the HKY+gamma model from these alignments with gap positions removed. By construction, the miRNAs are more conserved than even the protein coding sequences, but with pig and human being phylogenetically closest. For the 50 triple-alignments, we obtained 25 cases where pig is closer to human than to mouse, 2 cases where pig is closer to mouse than to human, and 23 cases where pig is equally distant to human and mouse.
Average GC content and the variance among alignments exceeding 40 bp for each species and each functional category. Variance is standardized to the variance observed in the human sequence.
Mean GC content
Variance GC content
Type of sequence
Even though divergence between pig and human occurred approximately at the same time as the divergence between human and mouse, the pig sequence is much more similar to the human sequence. Thus, the availability of the pig sequence effectively subdivides the human-mouse evolutionary branch at a position closest to human. This implies that one can determine which changes occurred on the human and mouse branches, respectively, since their divergence. The results of the phylogenetic analysis show that the relative length of the mouse, human and pig branches are different for the different types of data. Intronic sites and intergenic sites show a similar pattern, which also closely resembles that of synonymous sites [see ], reflecting that purifying selection is probably weak and similar for these regions. It is noteworthy that intergenic branch lengths are slightly shorter than intronic ones (and both are shorter than synonymous sites, see ). This may reflect either 1) more selective constraints on intergenic sequences than intronic, i.e. parts of the annotated intergenic sequences are indeed genic, or 2) a bias in the construction of the human-mouse alignment that make it easier to align sequence close to the conserved exons so that somewhat more divergent intronic sequence can be detected. The differences in evolutionary rates among the three species are most likely attributable to differences in generation times since they diverged. Non-synonymous sites show shorter branches that are much more similar in lengths among the species . This reflects purifying selection, which has been strongest in the mouse lineage, followed by pig and then human [20, 21]. The reason for this may be the larger average population size in the mouse since divergence. The UTR regions shows more selective constraint than introns.
The similarity between human and pig adds to the recently reported ultra-conserved regions . Pig and human share more ultra-conserved regions than human and mouse, and (correcting for the coverage in the data presented) virtually all of the ultra-conserved elements defined by Bejerano et al.  are also found in pig and therefore most likely in the artiodactyls lineage.
Variation in GC content along the genome (isochore structure) is more pronounced in primates than in rodents [4, 5]. The present results extend these findings and put artiodactyls in line with primates – lending further support to the suggestion that isochore evolution in rodents deviates from all other lineages, possibly because of extensive genome rearrangements .
A 0.66X coverage pig genome survey is hereby released. Even though it is only a beginning, the data offer many analytical possibilities and should also stimulate the international initiatives to generate a complete draft of the pig genome. The initial analysis of the data adds to our understanding of the evolutionary relationships of humans, mice and pigs. Further comparative genomic studies and more detailed genetic analyses will greatly improve our ability to elucidate pig as well as human biology and medicine.
Generation of pig shot-gun sequences
Genomic DNA was extracted from blood samples from 5 different breeds of Sus scrofa (domestic pig): ErHuaLian, Duroc, Landrace, Yorkshire and Hampshire. Following mechanical shearing, DNA fragments (1–3 kb) were isolated by gel electrophoresis and cloned into the SmaI restriction site of the pUC18 plasmid using blunt-end ligation. After transformation into E. coli (strain DH5a) and selection on LB-plates, individual clones were picked for the library. The quality of a library was checked by sequencing a small number of plasmids, which were assembled by phrap and aligned by BLAST to validate the randomness of the library and the proportion of contaminations – including mitochondrial DNA, human fragments and vector sequences. If the library was eligible, plasmid DNA was extracted from the individual clones using the membrane-filter method (Millipore). The pig genomic inserts were sequenced using the M13 bidirectional sequencing primers on the MegaBACE1000 platform using ET dye terminator. The chromatograms were registered in a relational database tracking all generated data to eliminate duplicated work and check for errors. Using phred v. 0.020425.c with a quality cut-off of 0.05 and the -trim_alt parameter the chromatograms were traced and the resulting traces were masked for vector sequences using CROSSMATCH (0.990329). Resulting sequences were resubmitted to the relational database and prepared for submission. Following removal of contamination from vector and bacterial host, we retained 3,835,271 reads of at least 150 bp.
All sequences with trace files have been submitted to the Ensembl/NCBI Trace repository under the center name "SDJVP", and project name "Sino-Danish Pig Genome Project".
Standard masking of repetitive sequences was performed using Repeatmasker version 2004/03/06 with RepBase Update 8.12 with Sus scrofa as query species, using default settings.
Construction of three species alignments
The newest build of the human-mouse pairwise alignment (hg17/mm5) based on the improved blastZ algoritm  was downloaded from the UCSC genome browser  and the repeatmasked shotgun-sequences were BLAST'ed up against this alignment using Megablast  with the following settings: (w) word size = 12, (e) minimum e-value = 0.0001, (x) extension parameter = 50, (u) Repeatmasked query sequences = True. Resulting hits were then used as tags to build the alignment around (see below).
All query sequences with multiple blast hits on different parts of the human-mouse alignment (approximately 10%) were removed at this point and only the remaining pig sequences were used in the further analyses. This was done to conservatively eliminate paralogous hits. The individual blast hits were used to position the pig query sequences on a specific part of the human-mouse alignment. For each BLAST hit a region extending 300 base pairs in both directions was then realigned using DIALIGN version 2.2.1 with default parameters . Regions that afterwards were not considered by DIALIGN to be aligned were removed at this point. Subsequently an annotation file (refGene.txt) of the human genome (hg17) was downloaded from the UCSC genome server and used to annotate each position in the alignment according to the following classes 1) protein-coding exon (with reading frame position), 2) intron, 3) 5' UTR, 4) 3' UTR, and 5) intergenic. Phylogenetic analyses were performed on each of these classes separately.
Estimation of phylogenetic trees
A combined alignment for each of the five functional classes was constructed by concatenating the many small three species alignments, and gap positions were removed. For each class of data (exon, 5'UTR, 3'UTR, intron and intergenic), the implementation of the HKY85+GAMMA model  in PAML v. 3.14  was used to estimate the number of substitutions in each evolutionary branch.
Analysis of ultra-conserved regions
The data set containing the 481 ultra-conserved regions defined by Bejerano et al.  was downloaded and used for the first part of this analysis. We estimated how many of these regions that can be found in the pig genome shotgun sequences by a simple BLAST (blastn) approach retaining only hits of a length of 150 bp or more. Since these ultra-conserved regions are based on external data, artefacts due to the relatively low coverage of the pig genome sequences are not important here.
In the second part of the analysis (searching for novel ultra-conserved regions), it was important to address the artefacts that arise from searching for ultra conserved regions in a fragmented dataset. In order to do a comparable search for ultra-conserved regions against the mouse and pig genomes, we artificially created a mouse data set resembling the fragmented pig shotgun data set. Since the identification criteria we use is 100% conservation over 200 bp or more, the length of the individual read as well as the quality become a major factor. The fragmented mouse data set was generated by downloading all ~79 million reads of the NCBI trace database, shuffle these randomly, and pick single reads until the same amount of nucleotides was reached as in the pig genomic reads dataset. Only pig reads with length > 200 bp were used and the data set consisted of 2,034,999,640 bp from pig and 2,665,153 reads (2,034,999,649 bp) from mouse.
Construction of the miRNA data set
The reads were BLAST searched  (default options) against the miRNA hairpin database  and matches of at least 60 nucleotides clustered. The obtained set of 219 clusters contained redundancy as the same pig reads hit the same miRNAs from different species (human, mouse, etc.). This could be converted into a unique set of 68 clusters (each containing 2–5 reads), i.e. each type of miRNA is now only represented once. Each cluster was assembled with Cap3  from TGICL . As a result 84 contigs and singletons were obtained and BLAST searched back against the miRNA database. Matches with more than 95% identity in an alignment length larger than 95% of the miRNA hairpin length were selected. This resulted in 54 hairpins complying with the criteria defined in Ambros et al. . In total 51 different mature sequences are covered in the 54 hairpins.
This study was supported by grants from: Danish National Committee for Pig Production, Danish Trans-Disciplinary Research Programme: "Comparative Genomics Focusing on Man and Pig", Danish National Research Foundation: "Danish Platform for Integrative Biology", The Danish Technical Research Council, Danish Center for Scientific Computing, Chinese Academy of Sciences, Chinese Ministry of Science and Technology, Natural Science Foundation of China. We acknowledge the Sino-Danish Pig Genome Consortium consisting of The Danish Veterinary and Agricultural University (KVL), Denmark, the Danish Institute of Agricultural Sciences (DIAS), Denmark, and the Beijing Genomics Institute/James D. Watson Institute of Genome Sciences (BGI/WIGS), China.
- Blakesley RW, Hansen NF, Mullikin JC, Thomas PJ, McDowell JC, Maskeri B, Young AC, Benjamin B, Brooks SY, Coleman BI, Gupta J, Ho SL, Karlins EM, Maduro QL, Stantripop S, Tsurgeon C, Vogt JL, Walker MA, Masiello CA, Guan X, Bouffard GG, Green ED: An intermediate grade of finished genomic sequence suitable for comparative analyses. Genome Research. 2004, 14: 2235-2244. 10.1101/gr.2648404.PubMedPubMed CentralView ArticleGoogle Scholar
- Thomas JW, Touchman JW, Blakesley RW, Bouffard GG, Beckstrom-Sternberg SM, Margulies EH, Blanchette M, Siepel AC, Thomas PJ, McDowell JC, Maskeri B, Hansen NF, Schwartz MS, Weber RJ, Kent WJ, Karolchik D, Bruen TC, Bevan R, Cutler DJ, Schwartz S, Elnitski L, Idol JR, Prasad AB, Lee-Lin SQ, Maduro VV, Summers TJ, Portnoy ME, Dietrich NL, Akhter N, Ayele K, Benjamin B, Cariaga K, Brinkley CP, Brooks SY, Granite S, Guan X, Gupta J, Haghighi P, Ho SL, Huang MC, Karlins E, Laric PL, Legaspi R, Lim MJ, Maduro QL, Masiello CA, Mastrian SD, McCloskey JC, Pearson R, Stantripop S, Tiongson EE, Tran JT, Tsurgeon C, Vogt JL, Walker MA, Wetherby KD, Wiggins LS, Young AC, Zhang LH, Osoegawa K, Zhu B, Zhao B, Shu CL, De Jong PJ, Lawrence CE, Smit AF, Chakravarti A, Haussler D, Green P, Miller W, Green : Comparative analyses of multi-species sequences from targeted genomic regions. Nature. 2003, 424: 788-793. 10.1038/nature01858.PubMedView ArticleGoogle Scholar
- Waterston RH, Lindblad-Toh K, Birney E, Rogers J, Abril JF, Agarwal P, Agarwala R, Ainscough R, Alexandersson M, An P, Antonarakis SE, Attwood J, Baertsch R, Bailey J, Barlow K, Beck S, Berry E, Birren B, Bloom T, Bork P, Botcherby M, Bray N, Brent MR, Brown DG, Brown SD, Bult C, Burton J, Butler J, Campbell RD, Carninci P, Cawley S, Chiaromonte F, Chinwalla AT, Church DM, Clamp M, Clee C, Collins FS, Cook LL, Copley RR, Coulson A, Couronne O, Cuff J, Curwen V, Cutts T, Daly M, David R, Davies J, Delehaunty KD, Deri J, Dermitzakis ET, Dewey C, Dickens NJ, Diekhans M, Dodge S, Dubchak I, Dunn DM, Eddy SR, Elnitski L, Emes RD, Eswara P, Eyras E, Felsenfeld A, Fewell GA, Flicek P, Foley K, Frankel WN, Fulton LA, Fulton RS, Furey TS, Gage D, Gibbs RA, Glusman G, Gnerre S, Goldman N, Goodstadt L, Grafham D, Graves TA, Green ED, Gregory S, Guigo R, Guyer M, Hardison RC, Haussler D, Hayashizaki Y, Hillier LW, Hinrichs A, Hlavina W, Holzer T, Hsu F, Hua A, Hubbard T, Hunt A, Jackson I, Jaffe DB, Johnson LS, Jones M, Jones TA, Joy A, Kamal M, Karlsson EK, Karolchik D, Kasprzyk A, Kawai J, Keibler E, Kells C, Kent WJ, Kirby A, Kolbe DL, Korf I, Kucherlapati RS, Kulbokas EJ, Kulp D, Landers T, Leger JP, Leonard S, Letunic I, Levine R, Li J, Li M, Lloyd C, Lucas S, Ma B, Maglott DR, Mardis ER, Matthews L, Mauceli E, Mayer JH, McCarthy M, McCombie WR, McLaren S, McLay K, McPherson JD, Meldrim J, Meredith B, Mesirov JP, Miller W, Miner TL, Mongin E, Montgomery KT, Morgan M, Mott R, Mullikin JC, Muzny DM, Nash WE, Nelson JO, Nhan MN, Nicol R, Ning Z, Nusbaum C, O'Connor MJ, Okazaki Y, Oliver K, Overton-Larty E, Pachter L, Parra G, Pepin KH, Peterson J, Pevzner P, Plumb R, Pohl CS, Poliakov A, Ponce TC, Ponting CP, Potter S, Quail M, Reymond A, Roe BA, Roskin KM, Rubin EM, Rust AG, Santos R, Sapojnikov V, Schultz B, Schultz J, Schwartz MS, Schwartz S, Scott C, Seaman S, Searle S, Sharpe T, Sheridan A, Shownkeen R, Sims S, Singer JB, Slater G, Smit A, Smith DR, Spencer B, Stabenau A, Stange-Thomann N, Sugnet C, Suyama M, Tesler G, Thompson J, Torrents D, Trevaskis E, Tromp J, Ucla C, Ureta-Vidal A, Vinson JP, Von Niederhausern AC, Wade CM, Wall M, Weber RJ, Weiss RB, Wendl MC, West AP, Wetterstrand K, Wheeler R, Whelan S, Wierzbowski J, Willey D, Williams S, Wilson RK, Winter E, Worley KC, Wyman D, Yang S, Yang SP, Zdobnov EM, Zody MC, Lander ES, Mouse Genome Sequencing Consortium: Initial sequencing and comparative analysis of the mouse genome. Nature. 2002, 420: 520-562. 10.1038/nature01262.PubMedView ArticleGoogle Scholar
- Rat Genome Sequencing project consortium: Genome sequence of the Brown Norway rat yields insight into mammalian evolution. Nature. 2004, 428: 493-521. 10.1038/nature02426.View ArticleGoogle Scholar
- The international Chimpanzee Chromosome 22 Consortium: DNA sequence and comparative analysis of chimpanzee chromosome 22. Nature. 2004, 429: 382-388. 10.1038/nature02564.View ArticleGoogle Scholar
- Springer MS, Murphy WJ, Eizirik E, O'Brien SJ: Placental mammal diversification and the Cretaceous-Tertiary boundary. Proc Natl Acad Sci USA. 2003, 100: 1056-1061. 10.1073/pnas.0334222100.PubMedPubMed CentralView ArticleGoogle Scholar
- Jørgensen FG, Hobolth A, Jensen HH, Bendixen C, Fredholm M, Schierup MH: Comparative analysis of protein coding sequences from Human, Mouse and the domesticated Pig. BMC Biology. 2005, 3: 2-10.1186/1741-7007-3-2.PubMedPubMed CentralView ArticleGoogle Scholar
- Schwartz SW, Kent J, Smit A, Zhang Z, Baertsch R, Hardison RC, Haussler D, Miller W: Human-Mouse Alignments with BLASTZ. Genome Res. 2003, 13: 103-107. 10.1101/gr.809403.PubMedPubMed CentralView ArticleGoogle Scholar
- Kent WJ, Sugnet C, Furey T, Roskin K, Pringle T, Zahler A, Haussler D: The human genome browser at UCSC. Genome Res. 2002, 12: 996-1006. 10.1101/gr.229102. Article published online before print in May 2002.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang Z, Schwartz S, Wagner L, Miller W: A greedy algorithm for aligning DNA sequences. J Comput Biol. 2000, 7: 203-214. 10.1089/10665270050081478.PubMedView ArticleGoogle Scholar
- Morgenstern B: DIALIGN 2: Improvement of the segment-to-segment approach to multiple sequence alignment. Bioinformatics. 1999, 15: 211-218. 10.1093/bioinformatics/15.3.211.PubMedView ArticleGoogle Scholar
- Hasegawa M, Kishino H, Yano T: Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol. 1985, 22: 160-174.PubMedView ArticleGoogle Scholar
- Yang Z: PAML: A program package for phylogenetic analysis by maximum likelihood. Comput Appl Biosci. 1997, 13: 555-556.PubMedGoogle Scholar
- Bejerano G, Pheasant M, Makunin I, Stephen S, Kent WJ, Mattick JS, Haussler D: Ultraconserved elementsin the human genome. Science. 2004, 304: 1321-1325. 10.1126/science.1098119.PubMedView ArticleGoogle Scholar
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-3402. 10.1093/nar/25.17.3389.PubMedPubMed CentralView ArticleGoogle Scholar
- Griffiths-Jones S: The miRNA Registry. Nucleic Acids Res. 2004, D109-D111. 10.1093/nar/gkh023. 32, Database
- Huang X, Madan A: CAP3: A DNA sequence assembly program. Genome Res. 1999, 9: 868-877. 10.1101/gr.9.9.868.PubMedPubMed CentralView ArticleGoogle Scholar
- Pertea G, Huang X, Liang F, Antonescu V, Sultana R, Karamycheva S, Lee Y, White J, Cheung F, Parvizi B, Tsai J, Quackenbush J: TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets. Bioinformatics. 2003, 19: 651-652. 10.1093/bioinformatics/btg034.PubMedView ArticleGoogle Scholar
- Ambros V, Bartel B, Bartel DP, Burge CB, Carrington JC, Chen X, Dreyfuss G, Eddy SR, Griffiths-Jones S, Marshall M, Matzke M, Ruvkun G, Tuschl T: A uniform system for microRNA annotation. RNA. 2003, 9: 277-279. 10.1261/rna.2183803.PubMedPubMed CentralView ArticleGoogle Scholar
- Ohta T: An examination of the generation-time effect on molecular evolution. Proc Natl Acad Sci USA. 1993, 90: 10676-10680.PubMedPubMed CentralView ArticleGoogle Scholar
- Ohta T: Synonymous and nonsynonymous substitutions in mammalian genes and the nearly neutral theory. J Mol Evol. 1995, 40: 56-63. 10.1007/BF00166595.PubMedView ArticleGoogle Scholar
- Belle EM, Duret L, Galtier N, Eyre-Walker A: The decline of isochores in mammals: an assessment of the GC content variation along the mammalian phylogeny. J Mol Evol. 2004, 58: 653-660. 10.1007/s00239-004-2587-x.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.