Table 3 Primers used for RT-PCR amplification of cDNA fragments of FBXO12B, FBXO12D, Tcl1b3, Tcl1b4, Tcl1, SpeerA, AK076711, Oosp3 and actin.
Gene | Primers | annealing temperature | fragment size (pb) | |
|---|---|---|---|---|
FBXO12B | Upper | ATTTGCCTCGTTTGCCTCTGA | 49°C | 890 pb |
Lower | GGTTATCCTGTCTTCCCTTCT | |||
FBXO12D | Upper | AGCCCTGTCCTTTTCCTGTCA | 50°C | 491 pb |
Lower | AATAGTCTGGTTTCCCCTCAC | |||
Tcl1b3 | Upper | TGGTAGACAGTGGGTAGTTGC | 54°C | 585 pb |
Lower | CAGGTGGAGGAGTTTGAATGG | |||
Tcl1b4 | Upper | TAAGAAGGCAGCCAACCAGAC | 54°C | 838 pb |
Lower | CTACCCAGCACCAGGCGAACT | |||
Tcl1 | Upper | TTTTATCACGGACTGGCATTG | 54°C | 1208 pb |
Lower | CCCTCATTTATTGGCATCTCG | |||
SpeerA | Upper | TGAAGCAGGAAAATAGGAGA | 51°C | 900 pb |
Lower | CCAAACAGAAAACCAAACAC | |||
AK076711 | Upper | GGCTCCCAGAGGCTGAATCA | 61°C | 769 pb |
Lower | TAAGGGTGTCCAAAGAATCA | |||
Oosp3 | Upper | TATTTGGGATGTGGAGATGTT | 52°C | 416 pb |
Lower | TTGAGGAGGAGGGTGGAAGTC | |||
Actin | Upper | ACGGAACCACAGTTTATCATC | 60°C | 180 pb |
Lower | GTCCCAGTCTTCAACTATACC | |||