Table 3 Real-time RT-PCR confirmation of microarray results
From: Gene expression alterations in brains of mice infected with three strains of scrapie
Encoded protein/gene | Symbol | Microarray fold change | RT-PCR fold change* (P value) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Â | Â | ME7 | 22L | RML | Ave. | ME7 | 22L | RML | Ave. |
Diazepan binding inhibitor | Dbi | 1.2 | 1.5 | 1.7 | 1.5 | 2.0 (0.021) | 2.2 (0.009) | 1.9 (0.026) | 2.0 (0.001) |
CD9 antigen | Cd9 | 1.3 | 1.9 | 1.5 | 1.6 | 2.9 (0.015) | 4.2 (0.001) | 3.7 (0.013) | 3.6 (0.000) |
CathepsinB | Ctsb | 1.4 | 1.7 | 1.3 | 1.5 | 1.7 (0.019) | 2.0 (0.003) | 1.9 (0.009) | 1.9 (0.011) |
Chemokine (C-X3-C) receptor 1 | Cx3cr1 | 1.2 | 1.2 | 1.4 | 1.3 | 1.8 (0.107) | 3.2 (0.025) | 2.0 (0.068) | 2.4 (0.011) |
Atpase, Na+/K+ transporting, beta 1 | Atp1b | -1.6 | -1.3 | -1.2 | -1.4 | -1.3 (0.185) | -1.3 (0.195) | -1.3 (0.205) | -1.3 (0.02) |
Glial fibrillary acidic protein | Gfap | 4.9 | 11.7 | 4.3 | 6.3 | 11.6 (0.005) | 14.6 (0.003) | 12.3 (0.005) | 12.8 (0.000) |
Apolipoprotein E | Apoe | 1.9 | 1.8 | 2.0 | 1.9 | 2.2 (0.005) | 2.1 (0.013) | 2.7 (0.086) | 2.3 (0.002) |
Glyceraldehydes-3-phosphate dehydrogenase | Gapdh | NA | NA | NA | NA | -1.1 (0.619) | -1.0 (0.798) | -1.2 (0.445) | -1.1 (0.393) |
Actin, beta, cytoplasmic | Actb | 1.1 | 1.2 | 1.0 | 1.1 | 1.0 (0.829) | 1.3 (0.275) | 1.1 (0.693) | 1.1 (0.352) |