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Table 1 List of the Chlamydiales-specific proteins

From: BLAST screening of chlamydial genomes to identify signature proteins that are unique for the Chlamydiales, Chlamydiaceae, Chlamydophila and Chlamydia groups of species

Protein Loci

Protein (Name/Size)

C. tra

C. mur

C. pne

C. cav

C. abo

Protochlam.

Next best BLAST Hit (E value, protein length)

CT007 (AAC67597)

hypoth, 316aa

0

8e-164

8e-108

6e-108

3e-105

8e-10, 320aa

Plasmodium yoelii (0.063, 1491aa)

CT011 (AAC67601)

hypoth, 418aa

0

0

7e-151

2e-155

1e-157

7e-47, 445aa

Desulfotalea psychrophila (1e-06, 337aa)

CT016 (AAC67606)

hypoth, 242aa

2e-139

3e-119

1e-82

4e-89

3e-86

2e-37, 233aa

Rickettsia sibirica (0.20, 1851aa)

CT017 (AAC67607)

hypoth. 433aa

0

0

1e-122

7e-133

9e-135

4e-52, 431aa

Thermo. tengcongensis (1e-06, 306 aa)

CT021 (AAC67611)

hypoth, 247aa

2e-139

4e-107

1e-47

3e-56

4e-53

2e-13, 245aa

Xenopus laevis (1.7, 600aa)

CT031 (AAC67621)

hypoth, 100aa

4e-51

4e-42

2e-25

4e-26

2e-26

0.010, 107aa

Mus musculus (3.6, 384aa)

CT038 (AAC67628)

hypoth, 116aa

1e-63

2e-49

4e-25

1e-25

2e-23

2e-09, 109aa

Neurospora crassa (2.8, 1223aa)

CT053 (AAC67644)

hypoth, 148aa

2e-61

4e-57

2e-51

1e-50

1e-48

5e-29, 155aa

Xenopus tropicalis (4.7, 1049aa)

CT066 (AAC67657)

hypoth, 158aa

3e-77

1e-75

8e-65

3e-65

5e-65

6e-41, 165aa

Vibrio parahaemolyticus (1.8, 92aa)

CT079 (AAC67670)

hypoth, 147aa

4e-81

1e-71

2e-06

1e-29

1e-29

3e-06, 172aa

Leuconostoc mesenteroides (0.11, 263aa)

CT131 (AAC67722)

PTMP, 1138aa

0

0

0

0

0

3e-33, 1348aa

Plasmodium yoelii (0.31, 592aa)

CT181 (AAC67772)

hypoth, 236aa

7e-131

1e-113

3e-77

7e-81

4e-80

2e-10, 200aa

Acinetobacter sp. (0.94, 371aa)

CT260 (AAC67853)

hypoth, 163aa

9e-91

9e-81

5e-62

3e-68

1e-67

4e-22, 159aa

Sinorhizobium meliloti (0.18, 166aa)

CT266 (AAC67859)

hypoth, 393aa

0

5e-179

9e-103

5e-120

2e-119

4e-08, 498aa

Xanthomonas axonopodis (2.8, 189aa)

CT271 (AAC67864)

hypoth, 95aa

1e-39

2e-36

7e-25

1e-25

9e-26

1e-10, 97aa

Gibberella zeae (3.6, 1362)

CT273 (AAC67866)

hypoth, 188aa

2e-89

1e-77

2e-60

5e-63

2e-63

0.001, 149aa

Corynebacterium efficiens (0.34, 640aa)

CT274 (AAC67867)

type III secretion chaperone, 139aa

3e-74

3e-70

1e-61

1e-62

1e-61

3e-31, 131aa

Synechococcus elongatus (2e-05, 363aa)

CT303 (AAC67896)

hypoth, 171aa

2e-81

5e-66

2e-40

2e-47

6e-47

3e-04, 177aa

Yersinia pestis (1.3, 373aa)

CT309 (AAC67902)

hypoth, 266aa

2e-144

2e-129

8e-86

1e-93

5e-94

2e-29, 263aa

Methanococcus jannaschii (2.6, 434aa)

CT312 (AAC67905)

pred. ferredoxin, 123aa

6e-67

6e-54

1e-30

7e-36

1e-35

1e-04, 130aa

Pan troglodytes (1.2, 1524aa)

CT350 (AAC67945)

hypoth, 566aa

0

0

2e-141

3e-157

1e-157

2e-30, 561aa

Nostoc sp. PCC 7120 (7e-06, 1547aa)

CT351 (AAC67946)

hypoth, 697aa

0

0

0

0

0

5e-53, 720aa

Rattus norvegicus (3.4, 389aa)

CT355 (AAC67950)

hypoth, 353aa

0

3e-161

3e-101

8e-101

1e-102

1e-21, 375aa

Chromobacterium violaceum (0.82, 556aa)

CT384 (AAC67980)

hypoth, 539aa

0

0

9e-106

4e-113

1e-111

2e-14, 580aa

Pseudomonas fluorescens (0.29, 314aa)

CT387 (AAC67984)

hypoth, 691aa

0

0

0

0

0

4e-160, 740aa

Rubrobacter xylanophilus (5e-10, 263aa)

CT421.2 (AAC68031)

hypoth, 53aa

5e-23

2e-22

5e-19

7e-20

4e-19

2e-12, 52aa

no other BLAST hits

CT425 (AAC68022)

hypoth, 621aa

0

0

0

0

0

0, 665aa

Bordetella parapertussis (4e-10, 767aa)

CT429 (AAC68026)

hypoth, 329aa

4e-165

4e-162

5e-149

2e-151

7e-151

8e-96, 388aa

Geobacter sulfurreducens (2e-04, 146aa)

CT443 (AAC68042)

OmcB, 553aa

0

0

0

0

0

4e-66, 558aa

Rhodopirellula baltica (3e-25, 907aa)

CT446 (AAC68045)

EUO protein, 183aa

1e-78

8e-72

1e-50

2e-53

1e-52

4e-41, 139aa

Magnaporthe grisea (2.1, 1259aa)

CT474 (AAC68074)

LysM protein, 336aa

2e-168

9e-116

5e-59

9e-71

7e-71

3e-04, 482aa

no other BLAST hits

CT504 (AAC68105)

hypoth, 288aa

2e-127

1e-105

4e-64

2e-69

2e-68

1e-23, 376aa

Mus musculus (3.0, 961aa)

CT538 (AAC68140)

hypoth, 238aa

8e-124

1e-120

5e-105

1e-108

1e-108

9e-65, 244aa

Fritschea bemisiae (3e-59, 241aa) Enterococcus faecalis (0.067, 541aa)

CT546 (AAC68148)

predicted OMP, 289aa

3e-163

4e-157

6e-87

1e-92

1e-93

3e-23, 249aa

Legionella pneumophila (0.042, 254aa)

CT547 (AAC68149)

hypoth, 318aa

2e-175

7e-158

8e-90

1e-92

2e-93

8e-29, 468aa

Caenorhabditis elegans (4e-04, 1168aa)

CT548 (AAC68150)

hypoth, 194aa

3e-89

2e-67

1e-48

2e-49

3e-48

0.004, 185aa

Drosophila melanogaster (0.17, 826aa)

CT556 (AAC68158)

hypoth, 159aa

1e-92

1e-82

9e-59

1e-60

1e-59

2e-06, 157aa

Xenopus laevis (1.8, 1636aa)

CT560 (AAC68162)

hypoth, 278aa

1e-135

2e-125

1e-86

4e-94

5e-93

0.002, 281aa

no other BLAST hits

CT573 (AAC68175)

hypoth, 409aa

0

1e-179

9e-90

3e-113

2e-106

1e-43, 472aa

Pseudomonas syringae (0.46, 9376aa)

CT583 (AAC68185)

Virulence plasmid pGP6 related protein, 263aa

5e-134

6e-118

4e-82

4e-91

2e-92

1e-46, 245aa

Crocosphaera watsonii (0.036, 333aa)

CT590 (AAC68192)

hypoth, 954aa

0

0

0

0

0

8e-96, 989aa

Porphyromonas gingivalis (0.003, 645aa)

CT602 (AAC68205)

hypoth, 130aa

5e-60

1e-55

2e-35

3e-37

1e-37

2e-19, 131aa

Actinobacillus pleuropneumoniae (2.8, 300aa)

CT616 (AAC68220)

hypoth, 429aa

0

0

2e-101

8e-115

3e-114

2e-09, 435aa

Giardia lamblia (0.003, 1024aa)

CT623 (AAC68227)

76 kDa Homolog, 446aa

0

2e-155

5e-152

1e-154

1e-157

1e-28, 470aa

Mycobacterium tuberculosis (0.18, 550aa)

CT631 (AAC68235)

hypoth, 84aa

1e-32

2e-31

1e-24

3e-28

8e-28

1e-11, 82aa

No other BLAST hits

CT635 (AAC68239)

hypoth, 144aa

2e-62

5e-58

6e-45

1e-50

2e-51

4e-04, 146aa

Gallus gallus (0.003, 1748aa)

CT642 (AAC68246)

hypoth, 271aa

2e-134

2e-116

2e-74

2e-79

8e-81

2e-22, 300aa

Rattus norvegicus (1.6, 509aa)

CT652.1 (AAC68830)

hypoth, 59aa

5e-26

3e-24

7e-12

3e-12

7e-13

3e-05, 75aa

Parachlamydia sp. (0.12, 70aa)

CT666 (AAC68261)

hypoth, 83aa

5e-40

9e-31

3e-35

1e-36

9e-37

0.43, 92aa

Sacchromyces cerevisiae (3.7, 604aa)

CT667 (AAC68262)

hypoth, 149aa

3e-78

1e-65

3e-51

9e-55

4e-55

8e-16, 154aa

Legionella pneumophila (0.005, 577aa)

CT670 (AAC68265)

hypoth, 168aa

1e-29

1e-27

2e-23

4e-24

3e-23

9e-10, 165aa

No other BLAST hits

CT734 (AAC68329)

hypoth, 221aa

7e-106

1e-95

2e-51

1e-56

2e-57

1e-08, 240aa

Caenorhabditis briggsae (1.1, 1072aa)

CT764 (AAC68359)

hypoth, 268aa

2e-154

4e-113

1e-46

7e-59

4e-59

2e-05, 256aa

Tetraodon nigroviridis (1.5, 388aa)

CT788 (AAC68383)

leader (60) peptide-periplasmic, 166aa

1e-79

3e-61

9e-25

4e-34

1e-32

0.003, 158aa

Caenorhabditis elegans (0.19, 2271aa)

CT809 (AAC68405)

hypoth, 103aa

1e-55

2e-44, 146aa

5e-17

2e-23

2e-21

4e-05, 158aa

Canis familiaris (2.1, 1831aa)

CT838 (AAC68435)

YjgP/YjgQ family protein, 366aa

0

0

e-126

e-130

3e-128

6e-24, 360aa

Prochlorococcus marinus (0.18, 592aa)

CT845 (AAC68442)

hypoth, 92aa

2e-46

7e-33

1e-19

2e-17

1e-17

8e-10, 85aa

Candida albicans (2.7, 557aa)

CT849.1 (AAC68455)

hypoth, 62aa

2e-28

9e-24

5e-10

2e-09

1e-08

1e-05, 81aa

No other BLAST hits

CT865 (AAC68463)

hypoth, 329aa

2e-180

8e-146

7e-76

1e-77

4e-78

9e-23, 352aa

Bacteroides thetaiotaomicron (0.20, 808aa)

  1. The Chlamydiales-specific proteins were identified by systematic BLASTP searches using Chl. trachomatis ORFs as probes, where the only significant BLAST hits obtained were for chlamydiae species (see Methods section). Accession numbers of the query sequences are shown beside the Chl. trachomatis loci (CT number) in the first column. The observed expected (E) values for various chlamydiae homologs as well as the first non-chlamydial hit in the BLAST results are given. E values of 0.0 indicate an extremely high degree of similarity between proteins sequences. The second column describes the function and the size of each query protein (protein sizes are described for other chlamydiae only if they noticeably differ from the query protein). Abbreviations are: C. tra, Chl. trachomatis; C. mur; Chl. muridarum; C. pne, Chlam. pneumoniae; C. cav, Chlam. caviae; C. abo, Chlam. abortus; hypoth, hypothetical protein; Omc; Outer membrane cytochrome; OMP, outer membrane protein; Protochlam, Protochlamydia; Pred., Predicted; PTMP, Possible transmembrane protein.