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Table 2 A Listing of the Identified Chlamydiaceae-specific proteins

From: BLAST screening of chlamydial genomes to identify signature proteins that are unique for the Chlamydiales, Chlamydiaceae, Chlamydophila and Chlamydia groups of species

Protein Loci

Protein (Name/Size)

C. tra

C. mur

C. pne

C. cav

C. abo

Next best BLAST hit (E value, protein length)

CT006 (AAC67596)

hypoth, 189aa

3e-95

6e-72

3e-12

9e-10

2e-09

Listeria anguillarum (0.45, 314aa)

CT005 (AAC67595)

hypoth, 363aa

1e-117

4e-63

3e-18, 417aa

8e-20

1e-20

Ashbya gossypii (0.86, 2272aa)

CT018 (AAC 67608)

hypoth, 157aa

3e-89

5e-53

2e-10

8e-09

3e-06

Acetabularia acetabulum (3.9, 613aa)

CT046 (AAC67637)

Histone-like protein HC2, 203aa

2e-29

2e-25

2e-05, 172aa

8e-08, 152aa

2e-08

No other BLAST hits

CT080 (AAC67671)

LtuB protein, 97aa

3e-50

4e-37

3e-11

2e-13

9e-16

Clostridium tetani (0.73, 311aa)

CT082 (AAC67673)

hypoth, 560aa

0

0

7e-91

8e-103

2e-102

Saccharomycecs cerevisiae (2.0, 1537aa)

CT083 (AAC67674)

hypoth, 160aa

1e-71

1e-57

7e-30

4e-29

9e-25

Pseudomonas putida (0.17, 1162aa)

CT109 (AAC67700)

hypoth, 262aa

2e-149

9e-133

8e-66

4e-70

7e-70

Plasmodium falciparum (1.1, 1057aa)

CT142 (AAC67733)

hypoth, 285aa

3e-155

2e-109

1e-39

9e-50

6e-49

Heterobasidion annosum (5.0, 507aa)

CT143 (AAC67734)

hypoth, 280aa

3e-165

3e-123

2e-36

9e-55

4e-56

porcine (0.26, 725aa)

CT144 (AAC67735)

hypoth, 285aa

2e-143

3e-101

7e-28

9e-34

6e-31

Staphylococcus aureus (0.041, 154aa)

CT147 (AAC67738)

hypoth, 1449aa

0

0

3e-71

4e-88

4e-91

Homo sapiens (8e-10, 2442aa)

CT178 (AAC67769)

hypoth, 404aa

0

0

3e-114

3e-120

4e-122

Thiobacillus denitrificans (2e-15, 830aa)

CT195 (AAC67787)

hypoth, 363aa

0

2e-147

8e-55

4e-71

1e-68

Escherichia coli (1.5, 346aa)

CT232 (AAC67824)

IncB, 115aa

7e-45

1e-29

4e-11

2e-08

7e-10

Canis familiaris (0.59, 472aa)

CT233 (AAC67825)

IncC, 178aa

9e-75

3e-38

0.52, 203aa

4e-04

0.006,184aa

Tetraodon nigroviridis (0.5, 1010aa)

CT253 (AAC67846)

hypoth, 215aa

5e-108

2e-99

2e-67

5e-72

8e-73

Schizosaccharomyces pombe (0.12, 880aa)

CT263 (AAC67856)

hypoth, 196aa

7e-109

1e-72

5e-23

2e-31

1e-29

Mycobacterium bovis (2.4, 744aa)

CT288 (AAC67881)

hypoth, 563aa

0

3e-175

6e-16

5e-23

1e-25

Debaryomyces hansenii (4e-04, 2042aa)

CT289 (AAC67882)

hypoth, 379aa

3e-167

3e-95

6e-27

6e-38

5e-31

Streptococcus pyogenes (0.049, 535aa)

CT324 (AAC67917)

hypoth, 303aa

1e-161

8e-115

1e-50

6e-55

1e-55

Brucella melitensis (0.29, 263aa)

CT330 (AAC67925)

hypoth, 90aa

2e-44

1e-32

3e-07

3e-04

1e-05

Oryza sativa (2.7, 677aa)

CT338 (AAC67933)

hypoth, 153aa

1e-86

1e-66

1e-23

2e-29

5e-28

Acinetobacter sp. (1.6, 304aa)

CT365 (AAC67961)

hypoth, 575aa

0

4e-156

3e-58

7e-63

1e-61

No other BLAST hits

CT372 (AAC67968)

hypoth, 442aa

0

0

1e-114

2e-116

3e-119

Bradyrhizobium japonicum (0.016, 675aa)

CT377 (AAC67973)

LtuA, 46aa

4e-20

8e-19

2e-08

6e-11

5e-10

No other BLAST hits

CT383 (AAC67979)

hypoth, 243aa

7e-140

1e-88

3e-26

2e-35

2e-28

Burkholderia fungorum (2.9, 780aa)

CT392 (AAC67989)

yprS, 377aa

0

2e-116

1e-07

1e-14

3e-07

Saccharomyces cerevisiae (0.048, 1038aa)

CT412 (AAC68009)

PmpA, 975aa

0

0

8e-141

3e-179

4e-175

many other chlamydiae hits in between Chloroflexus aurantiacus (3e-05, 767aa)

CT413 (AAC68010)

PmpB, 1751aa

0

0

0

0

0

Chloroflexus aurantiacus, 8e-10, 767aa

CT421.1 (AAC68030)

hypoth, 53aa

7e-23

3e-20

4e-15

6e-12

1e-14

Bradyrhizobium japonicum (3.8, 803aa)

CT440 (AAC68039)

hypoth, 112aa

1e-58

2e-45

6e-08

1e-04

4e-07

Homo sapiens (0.017, 100aa) (partial)

CT442 (AAC68041)

15kDa SRP, 150aa

7e-70

1e-33

1e-04

1e-12

3e-12

No other BLAST hits

CT444 (AAC68043)

OmcA, 88aa

1e-39

4e-31

2e-12

6e-15

6e-15

Ovis aries (0.14, 197aa) **

CT466 (AAC68066)

hypoth, 109aa

5e-48

7e-39

2e-24

1e-26

1e-27

Geobacter metallireducens (0.55, 557aa)

CT471 (AAC68071)

hypoth, 200aa

5e-112

1e-103

1e-46

1e-62

5e-63

Azoarcus sp. (1.9, 168aa)

CT482 (AAC68082)

hypoth, 217aa

1e-115

1e-91

1e-49

6e-60

4e-64

Legionella pneumophila (1.8, 438aa)

CT483 (AAC68083)

hypoth, 121aa

1e-51

1e-36

6e-06

2e-08

4e-08

Rubrobacter xylanophilus (0.14, 293aa)

CT529 (AAC68131)

hypoth, 298aa

1e-151

2e-91

4e-36

1e-38

8e-42

Aspergillus nidulans (1.9, 274aa)

CT552 (AAC68154)

hypoth, 135aa

6e-72

1e-41

5e-04

3e-06

7e-07

Guillardia theta (0.19, 263aa)

CT565 (AAC68167)

hypoth, 147aa

7e-79

1e-69

1e-41

3e-46

7e-48

Caenorhabditis elegans (0.11, 317aa)

CT566 (AAC68168)

hypoth, 330aa

8e-175

6e-141

8e-86

4e-89

1e-87

Vibrio vulnificus (0.26, 506aa)

CT567 (AAC68169)

hypoth, 174aa

1e-87

9e-61

5e-24

2e-17

2e-17

Takifugu rubripes (4.1, 1374aa)

CT568 (AAC68170)

hypoth, 151aa

2e-71

4e-48

2e-22

2e-18

5e-18

Giardia lamblia (1.6, 6739aa)

CT577 (AAC68179)

hypoth, 119aa

1e-60

3e-48

6e-16

3e-26

2e-26

Giardia lamblia (0.050, 551aa)

CT578 (AAC68180)

hypoth, 487aa

8e-175

3e-150

4e-81

2e-99

1e-103

Candida glabrata (0.023, 592aa)

CT579 (AAC68181)

hypoth, 439aa

3e-141

8e-115

2e-76

4e-77

1e-81

No other BLAST hits

CT589 (AAC68191)

hypoth, 602aa

0

0

1e-126

1e-145

3e-146

Bacillus subtilis (0.052, 605aa)

CT618 (AAC68222)

hypoth, 266aa

1e-142

6e-87

8e-10

4e-19, 306aa

5e-22

Drosophila melanogaster (0.11, 1868aa)

CT620 (AAC68224)

hypoth, 838aa

0

0

0

1e-180

2e-177

Borrelia burgdorferi (0.29, 735aa)

CT646 (AAC68823)

hypoth, 459aa

0

0

3e-60

1e-68

5e-66

Aspergillus nidulans (5.9, 629aa)

CT647 (AAC68824)

hypoth, 192aa

2e-98

5e-65

2e-18

1e-15

3e-13

Ciona intestinalis (1.8, 407aa)

CT651 (AAC68828)

hypoth, 608aa

0

0

1e-97

7e-97

3e-95

Bacteroides thetaiotaomicron (0.15, 1003aa)

CT654 (AAC68249)

hypoth, 162aa

5e-81

9e-75

1e-27

1e-32

1e-33

Mesocricetus auratus (2.5, 210aa)

CT668 (AAC68263)

hypoth, 223aa

1e-114

9e-93

6e-69

5e-78

1e-76

Bacillus halodurans (0.045, 324aa)

CT671 (AAC68266)

hypoth, 283aa

2e-149

1e-115

2e-63

2e-76

1e-72

Giberella zeae (0.053, 595aa)

CT681 (AAC68276)

MOMP, 393aa

0

7e-176

2e-139

3e-137

2e-136

No other BLAST hits except chlamydiae

CT695 (AAC68290)

hypoth, 398aa

0

7e-147

6e-12

2e-23

2e-22

Ustilago maydis (0.44, 417aa)

CT696 (AAC68291)

hypoth, 403aa

0

3e-179

6e-59

3e-70

2e-73

Mus musculus (0.031, 708aa)

CT712 (AAC68307)

hypoth, 390aa

0

0

8e-101

1e-129

1e-132

Mus musculus (0.086, 3159aa)

CT713 (AAC68308)

PorB, 340aa

0

0

5e-120

2e-133

5e-132

No other BLAST hits except chlamydiae

CT718 (AAC68313)

hypoth, 174aa

3e-93

8e-81

3e-44

2e-46

6e-47

Prochlorococcus marinus (0.82, 250aa)

CT744 (AAC68339)

phosphoprotein, 821aa

0

0

2e-138

1e-153

3e-160

Cytophaga hutchinsonii (0.38, 466aa)

CT753 (AAC68348)

hypoth, 74aa

3e-34

4e-27

1e-13, 76aa

2e-13

2e-13

Cycas necrotic stunt virus (1.3, 559)

CT763 (AAC68358)

hypoth, 139aa

6e-72

3e-52

2e-28

3e-23

2e-22

Drosophila pseudoobscura (2.6, 773aa)

CT795 (AAC68390)

hypoth, 163aa

1e-79

7e-46

1e-13

3e-13

2e-14

Pan troglodytes (0.52, 924aa)

CT812 (AAC68408)

OmpD, 1531aa

0

0

0

0

0

Rhodopirelulla baltica (2e-04, 703aa)

CT846 (AAC68443)

hypoth, 234aa

1e-123

1e-108

7e-67

3e-64

1e-66

Streptomyces coelicolor (2.1, 689aa)

CT847 (AAC68444)

hypoth, 172aa

2e-88

8e-73

1e-53

1e-53

2e-52

Saccharomyces cerevisiae (0.001, 1434aa)

CT848 (AAC68445)

hypoth, 168aa

1e-76

5e-67

2e-55

6e-54

8e-54

Drosophila pseudoobscura (0.34, 1000aa)

CT849 (AAC68446)

hypoth, 159aa

4e-74

1e-64

2e-57

1e-56

4e-57

Gallus gallus (0.034, 1546aa)

CT860 (AAC68458)

hypoth, 493aa

0

1e-132

2e-56

4e-73

4e-77

Leishmania major (0.26, 1346aa)

CT861 (AAC68459)

hypoth, 506aa

0

7e-172

6e-67

9e-74

5e-76

Vibrio fischeri (0.008, 1023aa)

CT863 (AAC68461)

hypoth, 482aa

0

2e-150

7e-82

2e-86

7e-90

Mus musculus (0.006, 1043aa)

CT869 (AAC68467)

OmpE, 964aa

0

0

9e-108

1e-119

8e-112

Escherichia coli (9e-05, 1250aa)

CT870 (AAC68468)

OmpF, 1034aa

0

0

1e-40, 931aa

2e-41

5e-46

long list of chlamydiae homologs ; Dictyostelium discoideum (4e-04, 485aa)

CT871 (AAC68469)

OmpG, 1013aa

0

0

4e-127

7e-180

2e-175

long list of chlamydiae homologs ; Desulfotalea psychrophila (8e-07, 2363aa)

CT872 (AAC68470)

OmpH, 1016aa

0

0

6e-152, 978aa

0

0

long list of chlamydiae homologs ; Crocosphaera watsonii (0.28, 822aa)

CT874 (AAC68472)

OmpI, 878aa

0

0

2e-81, 930aa

9e-73, 942aa

2e-77

long list of chlamydiae homologs ; Nostoc punctiforme (0.006, 1851aa)

  1. These proteins are uniquely found in various Chlamydiaceae species but not detected in Protochlamydia or other bacterial groups. Other details as in Table 1. **OmcA is an annotated ORF in the P. amoebophila genome, however it could not be detected using Chl. trachomatis as probe via BLASTp and so was not considered a Chlamydiales protein; SRP, sulfur rich protein.