# Centering, scaling, and transformations: improving the biological information content of metabolomics data

- Robert A van den Berg
^{1}Email author, - Huub CJ Hoefsloot
^{2}, - Johan A Westerhuis
^{2}, - Age K Smilde
^{1, 2}and - Mariët J van der Werf
^{1}

**7**:142

**DOI: **10.1186/1471-2164-7-142

© van den Berg et al; licensee BioMed Central Ltd. 2006

**Received: **20 February 2006

**Accepted: **08 June 2006

**Published: **08 June 2006

## Abstract

### Background

Extracting relevant biological information from large data sets is a major challenge in functional genomics research. Different aspects of the data hamper their biological interpretation. For instance, 5000-fold differences in concentration for different metabolites are present in a metabolomics data set, while these differences are not proportional to the biological relevance of these metabolites. However, data analysis methods are not able to make this distinction. Data pretreatment methods can correct for aspects that hinder the biological interpretation of metabolomics data sets by emphasizing the biological information in the data set and thus improving their biological interpretability.

### Results

Different data pretreatment methods, i.e. centering, autoscaling, pareto scaling, range scaling, vast scaling, log transformation, and power transformation, were tested on a real-life metabolomics data set. They were found to greatly affect the outcome of the data analysis and thus the rank of the, from a biological point of view, most important metabolites. Furthermore, the stability of the rank, the influence of technical errors on data analysis, and the preference of data analysis methods for selecting highly abundant metabolites were affected by the data pretreatment method used prior to data analysis.

### Conclusion

Different pretreatment methods emphasize different aspects of the data and each pretreatment method has its own merits and drawbacks. The choice for a pretreatment method depends on the biological question to be answered, the properties of the data set and the data analysis method selected. For the explorative analysis of the validation data set used in this study, autoscaling and range scaling performed better than the other pretreatment methods. That is, range scaling and autoscaling were able to remove the dependence of the rank of the metabolites on the average concentration and the magnitude of the fold changes and showed biologically sensible results after PCA (principal component analysis).

In conclusion, selecting a proper data pretreatment method is an essential step in the analysis of metabolomics data and greatly affects the metabolites that are identified to be the most important.

## Background

Functional genomics approaches are increasingly being used for the elucidation of complex biological questions with applications that range from human health [1] to microbial strain improvement [2]. Functional genomics tools have in common that they aim to measure the complete biomolecule response of an organism to the environmental conditions of interest. While transcriptomics and proteomics aim to measure all mRNA and proteins, respectively, metabolomics aims to measure all metabolites [3, 4].

In this paper, we discuss different properties of metabolomics data, how pretreatment methods influence these properties, and how the effects of the data pretreatment methods can be analyzed. The effect of data pretreatment will be illustrated by the application of eight data pretreatment methods to a metabolomics data set of *Pseudomonas putida* S12 grown on four different carbon sources.

### Properties of metabolome data

In metabolomics experiments, a snapshot of the metabolome is obtained that reflects the cellular state, or phenotype, under the experimental conditions studied [3]. The experiments that resulted in the data set used in this paper were conducted according to an experimental design. In an experimental design, the experimental conditions are purposely chosen to induce variation in the area of interest. The resulting variation in the metabolome is called induced biological variation.

However, other factors are also present in metabolomics data:

1. Differences in orders of magnitude between measured metabolite concentrations; for example, the average concentration of a signal molecule is much lower than the average concentration of a highly abundant compound like ATP. However, from a biological point of view, metabolites present in high concentrations are not necessarily more important than those present at low concentrations.

2. Differences in the fold changes in metabolite concentration due to the induced variation; the concentrations of metabolites in the central metabolism are generally relatively constant, while the concentrations of metabolites that are present in pathways of the secondary metabolism usually show much larger differences in concentration depending on the environmental conditions.

3. Some metabolites show large fluctuations in concentration under identical experimental conditions. This is called uninduced biological variation.

Besides these biological factors, other effects present in the data set are:

4. Technical variation; this originates from, for instance, sampling, sample work-up and analytical errors.

5. Heteroscedasticity; for data analysis, it is often assumed that the total uninduced variation resulting from biology, sampling, and analytical measurements is symmetric around zero with equal standard deviations. However, this assumption is generally not true. For instance, the standard deviation due to uninduced biological variation depends on the average value of the measurement. This is called heteroscedasticity, and it results in the introduction of additional structure in the data [6, 7]. Heteroscedasticity occurs in uninduced biological variation as well as in technical variation.

The variation in the data resulting from a metabolomics experiment is the sum of the induced variation and the total uninduced variation. The total uninduced variation is all the variation originating from uninduced biological variation, sampling, sample work-up, and analytical variation. Data pretreatment focuses on the biologically relevant information by emphasizing different aspects in the clean data, for instance, the metabolite concentration under a growth condition relative to the average concentration, or relative to the biological range of that metabolite. In metabolomics, data pretreatment relates the differences in metabolite concentrations in the different samples to differences in the phenotypes of the cells from which these samples were obtained [3].

### Data pretreatment methods

The choice for a data pretreatment method does not only depend on the biological information to be obtained, but also on the data analysis method chosen since different data analysis methods focus on different aspects of the data. For example, a clustering method focuses on the analysis of (dis)similarities, whereas principal component analysis (PCA) attempts to explain as much variation as possible in as few components as possible. Changing data properties using data pretreatment may therefore enhance the results of a clustering method, while obscuring the results of a PCA analysis.

Overview of the pretreatment methods used in this study. In the Unit column, the unit of the data after the data pretreatment is stated. *O* represents the original Unit, and (-) presents dimensionless data. The mean is estimated as: ${\overline{x}}_{i}=\frac{1}{J}{\displaystyle \sum _{j=1}^{J}{x}_{ij}}$ and the standard deviation is estimated as: ${s}_{i}=\sqrt{\frac{{\displaystyle \sum _{j=1}^{J}{\left({x}_{ij}-{\overline{x}}_{i}\right)}^{2}}}{J-1}}$. $\tilde{x}$ and $\widehat{x}$ represent the data after different pretreatment steps.

Class | Method | Formula | Unit | Goal | Advantages | Disadvantages |
---|---|---|---|---|---|---|

| Centering | ${\tilde{x}}_{ij}={x}_{ij}-{\overline{x}}_{i}$ |
| Focus on the differences and not the similarities in the data | Remove the offset from the data | When data is heteroscedastic, the effect of this pretreatment method is not always sufficient |

| Autoscaling | ${\tilde{x}}_{ij}=\frac{{x}_{ij}-{\overline{x}}_{i}}{{s}_{i}}$ | (-) | Compare metabolites based on correlations | All metabolites become equally important | Inflation of the measurement errors |

Range scaling | ${\tilde{x}}_{ij}=\frac{{x}_{ij}-{\overline{x}}_{i}}{\left({x}_{{i}_{\mathrm{max}}}-{x}_{{i}_{\mathrm{min}}}\right)}$ | (-) | Compare metabolites relative to the biological response range | All metabolites become equally important. Scaling is related to biology | Inflation of the measurement errors and sensitive to outliers | |

Pareto scaling | ${\tilde{x}}_{ij}=\frac{{x}_{ij}-{\overline{x}}_{i}}{\sqrt{{s}_{i}}}$ |
| Reduce the relative importance of large values, but keep data structure partially intact | Stays closer to the original measurement than autoscaling | Sensitive to large fold changes | |

Vast scaling | ${\tilde{x}}_{ij}=\frac{\left({x}_{ij}-{\overline{x}}_{i}\right)}{{s}_{i}}\cdot \frac{{\overline{x}}_{i}}{{s}_{i}}$ | (-) | Focus on the metabolites that show small fluctuations | Aims for robustness, can use prior group knowledge | Not suited for large induced variation without group structure | |

Level scaling | ${\tilde{x}}_{ij}=\frac{{x}_{ij}-{\overline{x}}_{i}}{{\overline{x}}_{i}}$ | (-) | Focus on relative response | Suited for identification of e.g. biomarkers | Inflation of the measurement errors | |

| Log transformation | $\begin{array}{l}{\tilde{x}}_{ij}{=}^{10}\mathrm{log}\left({x}_{ij}\right)\\ {\stackrel{\u2322}{x}}_{ij}={\tilde{x}}_{ij}-{\overline{\tilde{x}}}_{i}\end{array}$ | Log | Correct for heteroscedasticity, pseudo scaling. Make multiplicative models additive | Reduce heteroscedasticity, multiplicative effects become additive | Difficulties with values with large relative standard deviation and zeros |

Power transformation | $\begin{array}{l}{\tilde{x}}_{ij}=\sqrt{\left({x}_{ij}\right)}\\ {\stackrel{\u2322}{x}}_{ij}={\tilde{x}}_{ij}-{\overline{\tilde{x}}}_{i}\end{array}$ |
| Correct for heteroscedasticity, pseudo scaling | Reduce heteroscedasticity, no problems with small values | Choice for square root is arbitrary. |

#### Class I: Centering

Centering converts all the concentrations to fluctuations around zero instead of around the mean of the metabolite concentrations. Hereby, it adjusts for differences in the offset between high and low abundant metabolites. It is therefore used to focus on the fluctuating part of the data [8, 9], and leaves only the relevant variation (being the variation between the samples) for analysis. Centering is applied in combination with all the methods described below.

#### Class II: Scaling

Scaling methods are data pretreatment approaches that divide each variable by a factor, the scaling factor, which is different for each variable. They aim to adjust for the differences in fold differences between the different metabolites by converting the data into differences in concentration relative to the scaling factor. This often results in the inflation of small values, which can have an undesirable side effect as the influence of the measurement error, that is usually relatively large for small values, is increased as well.

There are two subclasses within scaling. The first class uses a measure of the data dispersion (such as, the standard deviation) as a scaling factor, while the second class uses a size measure (for instance, the mean).

#### Scaling based on data dispersion

Scaling methods tested that use a dispersion measure for scaling were autoscaling [9], pareto scaling [10], range scaling [11], and vast scaling [12] (Table 1). Autoscaling, also called unit or unit variance scaling, is commonly applied and uses the standard deviation as the scaling factor [9]. After autoscaling, all metabolites have a standard deviation of one and therefore the data is analyzed on the basis of correlations instead of covariances, as is the case with centering.

Pareto scaling [10] is very similar to autoscaling. However, instead of the standard deviation, the square root of the standard deviation is used as the scaling factor. Now, large fold changes are decreased more than small fold changes, thus the large fold changes are less dominant compared to clean data. Furthermore, the data does not become dimensionless as after autoscaling (Table 1).

Vast scaling [12] is an acronym of *va* riable *st* ability scaling and it is an extension of autoscaling. It focuses on stable variables, the variables that do not show strong variation, using the standard deviation and the so-called coefficient of variation (cv) as scaling factors (Table 1). The cv is defined as the ratio of the standard deviation and the mean:$\frac{{s}_{i}}{{\overline{x}}_{i}}$. The use of the cv results in a higher importance for metabolites with a small relative standard deviation and a lower importance for metabolites with a large relative standard deviation. Vast scaling can be used unsupervised as well as supervised. When vast scaling is applied as a supervised method, group information about the samples is used to determine group specific cvs for scaling.

The scaling methods described above use the standard deviation or an associated measure as scaling factor. The standard deviation is, within statistics, a commonly used entity to measure the data spread. In biology, however, a different measure for data spread might be useful as well, namely the biological range. The biological range is the difference between the minimal and the maximal concentration reached by a certain metabolite in a set of experiments. Range scaling [11] uses this biological range as the scaling factor (Table 1). A disadvantage of range scaling with regard to the other scaling methods tested is that only two values are used to estimate the biological range, while for the standard deviation all measurements are taken into account. This makes range scaling more sensitive to outliers. To increase the robustness of range scaling, the range could also be determined by using robust range estimators.

#### Scaling based on average value

Level scaling falls in the second subclass of scaling methods, which use a size measure instead of a spread measure for the scaling. Level scaling converts the changes in metabolite concentrations into changes relative to the average concentration of the metabolite by using the mean concentration as the scaling factor. The resulting values are changes in percentages compared to the mean concentration. As a more robust alternative, the median could be used. Level scaling can be used when large relative changes are of specific biological interest, for example, when stress responses are studied or when aiming to identify relatively abundant biomarkers.

#### Class III: Transformations

Transformations are nonlinear conversions of the data like, for instance, the log transformation and the power transformation (Table 1). Transformations are generally applied to correct for heteroscedasticity [7], to convert multiplicative relations into additive relations, and to make skewed distributions (more) symmetric. In biology, relations between variables are not necessarily additive but can also be multiplicative [13]. A transformation is then necessary to identify such a relation with linear techniques.

Since the log transformation and the power transformation reduce large values in the data set relatively more than the small values, the transformations have a pseudo scaling effect as differences between large and small values in the data are reduced. However, the pseudo scaling effect is not determined by the multiplication with a scaling factor as for a 'real' scaling effect, but by the effect that these transformations have on the original values. This pseudo scaling effect is therefore rarely sufficient to fully adjust for magnitude differences. Hence, it can be useful to apply a scaling method after the transformation. However, it is not clear how the transformation and a scaling method influence each other with regard to the complex metabolomics data.

A transformation that is often used is the log transformation (Table 1). A log transformation perfectly removes heteroscedasticity if the relative standard deviation is constant [7]. However, this is rarely the case in real life situations. A drawback of the log transformation is that it is unable to deal with the value zero. Furthermore, its effect on values with a large relative analytical standard deviation is problematic, usually the metabolites with a relatively low concentration, as these deviations are emphasized. These problems occur because the log transformation approaches minus infinity when the value to be transformed approaches zero.

A transformation that does not show these problems and also has positive effects on heteroscedasticity is the power transformation (Table 1) [13]. The power transformation shows a similar transformation pattern as the log transformation. Hence, the power transformation can be used to obtain results similar as after the log transformation without the near zero artifacts, although the power transformation is not able to make multiplicative effects additive.

## Methods

### Background of the data set

*P. putida* S12 [14] is maintained at TNO. Cultures of *P. putida* S12 were grown in batch fermentations at 30°C in a Bioflow II (New Brunswick Scientific) bioreactor as previously described by van der Werf [15]. Samples (250 ml) were taken from the bioreactor at an OD 600 of 10. Cells were immediately quenched at -45°C in methanol as described previously [16]. Prior to extracting the intracellular metabolites from the cells – by chloroform extraction at -45°C [17] – internal standards were added [18] and a sample was taken for biomass determination [19]. Subsequently, the samples were lyophilized.

### GC-MS analysis

Lyophilized metabolome samples were derivatized using a solution of ethoxyamine hydrochloride in pyridine as the oximation reagent followed by silylation with N-trimethyl-N-trimethylsilylacetamide as described by [18]. GC-MS-analysis of the derivatized samples was performed using temperature gradient from 70°C to 320°C at a rate of 10°C/min on an Agilent 6890 N GC (Palo Alto, CA, USA) and an Agilent 5973 mass selective detector. 1 μl aliquots of the derivatized samples were injected in the splitless mode on a DB5-MS capillary column. Detection was performed using MS detection in electron impact mode (70 eV).

### Data preprocessing

^{3}. The output of the AMDIS analysis, in the form of peak identifiers and peak areas, was corrected for the recovery of internal standards and normalized with respect to biomass. The peaks resulting from a known compound were combined. The samples N3, S2 and S3 were removed from the data set, as a different sample workup protocol was followed. Furthermore, metabolites detected only once in the 13 remaining experiments were removed. This lead to a reduced data set consisting of 13 experiments and 140 variables expressed as peak areas in arbitrary units (Figure 2). This data set was used as the clean data for data pretreatment.

### Data pretreatment

Data pretreatment and PCA were performed using Matlab 7 [21], the PLS Toolbox 3.0 [22], and home written m-files. Data pretreatment was applied according to the formulas in Table 1. The notation of the formulas is as follows: Matrices are presented in bold uppercase (**X**), vectors in bold lowercase (**t**), and scalars are given in lowercase italic (*a*) or uppercase italic in case of the end of a series *i* = 1...*I*. The data is presented in a data matrix **X** (*I* × *J*) with *I* rows referring to the metabolites and *J* columns referring to the different conditions. Element *x*_{
ij
}therefore holds the measurement of metabolite *i* in experiment *j*.

Vast scaling was applied unsupervised as the other data pretreatment methods were unsupervised as well.

### Data analysis

PCA was applied for the analysis of the data. PCA decomposes the variation of matrix **X** into scores **T**, loadings **P**, and a residuals matrix **E**. **P** is an *I* × *A* matrix containing the *A* selected loadings and **T** is a *J* × *A* matrix containing the accompanying scores.

**X** = **PT**^{
T
}+ **E**,

where P^{
T
}P= I, the identity matrix.

The number of components used (*A*) in the PCA analysis was based on the scree plots and the score plots.

For ranking of the metabolites according to importance for the *A* selected PCs, the contribution *r* of all the variables to the effects observed in the *A* PCs was calculated

${r}_{Ai}={\displaystyle \sum _{a=1}^{A}{\lambda}_{a}^{2}\cdot {p}_{ia}^{2}}$

Here, *r* is the contribution of variable *i* to *A* components, λ_{
a
}is the singular value for the *a*^{th} PC and *p*_{
ia
}is the value for the *i*^{th} variable in the loading vector belonging to the *a*^{th} PC. To allow for comparison between the different data pretreatment methods, the values for *r*_{
A
}were sorted in descending order after which the comparisons were performed using the rank of the metabolite in the sorted list.

The measurement errors were analyzed by estimation of the standard deviation from the biological, analytical, and sampling repeats. The standard deviations were binned by calculating the average variance per 10 metabolites ordered by mean value [23].

The jackknife routine was performed according to the following setup. In round one experiments F1, G1, N1 were left out, in round two F2, G2, N1d were left out, and in round three F3, G3A, were left out. By selecting these experiments, the specific aspects of the experimental design were maintained.

## Results and discussion

### Properties of the clean data

Estimation of the sources of variation in the data set. The SS and the MS for the different sources of variation are given, based on the experimental design presented in Figure 2. *The technical source of variation consists of the analytical error and the sample work-up error.

Source of variation | SS | MS |
---|---|---|

Analytical | 0.0205 | 0.0102 |

Technical* | 0.0482 | 0.0482 |

Uninduced biological | 0.208 | 0.104 |

Induced biological | 0.952 | 0.317 |

Total SS | 1.23 |

### The effect of pretreatment on the clean data

### Heteroscedasticity

*relative*biological standard deviation (Figure 4C), and also the relative analytical standard deviation (unpublished results), showed the opposite effect. Thus, metabolites present in high concentrations were relatively influenced less by the disturbances resulting from the different sources of uninduced variation, and were therefore more reliable.

Scaling approaches influence the heteroscedasticity as well, since the variation, and thus the heteroscedasticity, is converted into relative values to the scaling factor. It is likely that this aspect reduces the effect of the heteroscedasticity on the results.

### The effect of data pretreatment on the data analysis results

PCA [9, 25] was applied to analyze the effect on the data analysis for the in different ways pretreated data. PCA was chosen as it is an explorative tool that is able to visualize how the data pretreatment methods are able to reveal different aspects of the data in the scores and the accompanying loadings. Furthermore, it allows for identification of the most important metabolites for the biological problem by analysis of the loadings.

The score plots were judged on two aspects by visual inspection, namely the distance within the cluster of a specific carbon source and the distance between the clusters of different carbon sources. The loading plots show the contributions of the measured metabolites to the separation of the experiments in the score plots. As cellular metabolism is strongly interlinked (e.g. see [26, 27]), it is expected that the concentrations of many metabolites are simultaneously affected when an organism is grown on a different carbon source. Therefore, the loadings are expected to show contributions of many different metabolites.

The application of centering lead to intermediate clustering results in the score plots (Figure 6B1). The clusters were larger and less well separated compared to the results for range scaling (Figure 6A1). The most striking results for centered data are visible in the loading plots (Figure 6B2 and 6B3). Only a few metabolites had very large contributions to the effects shown the score plot (Figure 6B1), which is in disagreement with the biological expectations. Power transformation and pareto scaling gave similar PCA results (unpublished results).

In contrast to the other pretreatment methods, vast scaling of the clean data resulted in a very poor clustering of the samples (Figure 6C1). Overlapping clusters were observed, although the loading plots (Figure 6C2 and 6C3) show contributions of many metabolites.

These results clearly demonstrate that the pretreatment method chosen dramatically influences the results of a PCA analysis. Consequently, these effects are also present in the rank of the metabolites.

### Ranking of the most important metabolites

^{st}, 11

^{th}, or 38

^{th}most important metabolite, respectively. The pretreatment of the clean data thus directly affected the ranking of the metabolites as being the most relevant.

### Reliability of the rank of the metabolites

While the rank of the metabolites provides valuable information, the robustness of this rank is just as important as it determines the limits of the reliable interpretation of the rank. To test the reliability of the rank of the metabolites, a jackknife routine was applied [28].

This resampling approach showed that the reliability of the rank of the most important metabolites is also dependent on the data pretreatment method. The most stable data pretreatment methods were centering, level scaling (Figure 9), log transformation, power transformation, pareto scaling, and vast scaling (results not shown). Autoscaling was less stable (results not shown), while the least stable data pretreatment method was range scaling. Two factors affect the reliability of the rank of the metabolites. The first factor relates to the reliability with which the scaling factor can be determined. For instance, level scaling uses the mean as the scaling factor. As the mean is based on all the measurements, it is quite stable. On the other hand, range scaling uses the biological range observed in the data as a scaling factor, which is based on two values only. The second factor that influences the reliability of the rank relates to those data pretreatment methods whose subsequent data analysis results show a preference for the high abundant metabolites (Figure 8). With these pretreatment methods, the stability of the rank is predetermined by this character due to the low relative standard deviation of the uninduced biological variation of the high abundant metabolites (Figure 4B).

It must be stressed that the pretreatment method that provides the most stable rank does not necessarily provides the most relevant biological answers.

## Conclusion

This paper demonstrates that the data pretreatment method used is crucial to the outcome of the data analysis of functional genomics data. The selection of a data pretreatment method depends on three factors: (i) the biological question that has to be answered, (ii) the properties of the data set, and (iii) the data analysis method that will be used for the analysis of the functional genomics data.

Notwithstanding these boundaries, autoscaling and range scaling seem to perform better than the other methods with regard to the biological expectations. That is, range scaling and autoscaling were able to remove the dependence of the rank of the metabolites on the average concentration and the magnitude of the fold changes and showed biologically sensible results after PCA analysis. Other methods showed a strong dependence on the average concentration or magnitude of the fold change (centering, log transformation, power transformation, level scaling, pareto scaling), or lead to PCA results that were poorly interpretable in relation to the experimental setup (vast scaling).

Using a pretreatment method that is not suited for the biological question, the data, or the data analysis method, will lead to poor results with regard to, for instance, the rank of the most relevant metabolites for the biological question that is subject of study (Figure 7 and 8). This will therefore result in a wrong biological interpretation of the results.

In functional genomics data analysis, data pretreatment is often overlooked or is applied in an ad hoc way. For instance, in many software packages, such as Cluster [29] and the PLS toolbox [22], data pretreatment is integrated in the data analysis program and can be easily turned on or off. This can lead to a careless search through different pretreatment methods until the results best fit the expectations of the researcher. Therefore, we advise against method mining. With method mining, the best result translates to 'which method fits the expectations the best'. This is poor practice, as results cannot be considered reliable when the assumptions and limitations of a data pretreatment method are not taken into account. Furthermore, it is sometimes unknown what to expect, or the starting hypothesis is incorrect.

As far as we are aware, this is the first time that the importance of selecting a proper data pretreatment method on the outcome of data analysis in relation to the identification of biologically important metabolites in metabolomics/functional genomics is clearly demonstrated.

## Declarations

### Acknowledgements

The authors would like to thank Karin Overkamp and Machtelt Braaksma for the generation of the biological samples and sample work up, and Maud Koek, Bas Muilwijk, and Thomas Hankemeier for the analysis of the samples and data preprocessing. This research was funded by the Kluyver Centre for Genomics of Industrial Fermentation, which is supported by the Netherlands Genomics Initiative (NROG).

## Authors’ Affiliations

## References

- Reis EM, Ojopi EPB, Alberto FL, Rahal P, Tsukumo F, Mancini UM, Guimaraes GS, Thompson GMA, Camacho C, Miracca E, Carvalho AL, Machado AA, Paquola ACM, Cerutti JM, da Silva AM, Pereira GG, Valentini SR, Nagai MA, Kowalski LP, Verjovski-Almeida S, Tajara EH, Dias-Neto E, Consortium HNA: Large-scale Transcriptome Analyses Reveal New Genetic Marker Candidates of Head, Neck, and Thyroid Cancer. Cancer Res. 2005, 65: 1693-1699. 10.1158/0008-5472.CAN-04-3506. [http://cancerres.aacrjournals.org/cgi/content/abstract/65/5/1693]PubMedView ArticleGoogle Scholar
- van der Werf MJ: Towards replacing closed with open target selection strategies. Trends Biotechnol. 2005, 23: 11-16. 10.1016/j.tibtech.2004.11.003.PubMedView ArticleGoogle Scholar
- van der Werf MJ, Jellema RH, Hankemeier T: Microbial Metabolomics: replacing trial-and-error by the unbiased selection and ranking of targets. J Ind Microbiol Biotechnol. 2005, 32: 234-252. 10.1007/s10295-005-0231-4. [http://dx.doi.org/10.1007/s10295-005-0231-4]PubMedView ArticleGoogle Scholar
- Fiehn O: Metabolomics - the link between genotypes and phenotypes. Plant Mol Biol. 2002, 48: 151-171. 10.1023/A:1013713905833.View ArticleGoogle Scholar
- Shurubor YI, Paolucci U, Krasnikov BF, Matson WR, Kristal BS: Analytical precision, biological variation, and mathematical normalization in high data density metabolomics. Metabolomics. 2005, 1: 75-85. 10.1007/s11306-005-1109-1.View ArticleGoogle Scholar
- Keller HR, Massart DL, Liang YZ, Kvalheim OM: Evolving factor analysis in the presence of heteroscedastic noise. Anal Chim Acta. 1992, 263: 29-36. 10.1016/0003-2670(92)85422-3.View ArticleGoogle Scholar
- Kvalheim OM, Brakstad F, Liang Y: Preprocessing of analytical profiles in the presence of homoscedastic or heteroscedastic noise. Anal Chem. 1994, 66: 43-51. 10.1021/ac00073a010.View ArticleGoogle Scholar
- Bro R, Smilde AK: Centering and scaling in component analysis. J Chemom. 2003, 17: 16-33. 10.1002/cem.773.View ArticleGoogle Scholar
- Jackson JE: Wiley series in probability and mathematical statistics. Applied probability and statistics. A user's guide to principal components. 1991, John Wiley & Sons, Inc.View ArticleGoogle Scholar
- Eriksson L, Johansson E, Kettaneh-Wold N, Wold S: Scaling. Introduction to multi- and megavariate data analysis using projection methods (PCA & PLS). 1999, Umetrics, 213-225.Google Scholar
- Smilde AK, van der Werf MJ, Bijlsma S, van der Werff-van der Vat B, Jellema RH: Fusion of mass-spectrometry-based metabolomics data. Anal Chem. 2005, 77: 6729-6736. 10.1021/ac051080y. [http://dx.doi.org/10.1021/ac051080y]PubMedView ArticleGoogle Scholar
- Keun HC, Ebbels TMD, Antti H, Bollard ME, Beckonert O, Holmes E, Lindon JC, Nicholson JK: Improved analysis of multivariate data by variable stability scaling: application to NMR-based metabolic profiling. Anal Chim Acta. 2003, 490: 265-276. 10.1016/S0003-2670(03)00094-1. [http://dx.doi.org/10.1016/S0003-2670(03)00094-1]View ArticleGoogle Scholar
- Sokal RR, Rohlf FJ: Assumptions of analysis of variance. Biometry. 1995, New York, W.H. Freeman and Co., 13: 392-450. 3rdGoogle Scholar
- Hartmans S, van der Werf MJ, de Bont JAM: Bacterial degradation of styrene involving a novel flavin adenine dinucleotide-dependent styrene monooxygenase. Appl Environ Microbiol. 1990, 56: 1347-1351.PubMedPubMed CentralGoogle Scholar
- van der Werf MJ, Pieterse B, van Luijk N, Schuren F, van der Werff-van der Vat B, Overkamp K, Jellema RH: Multivariate analysis of microarray data by principal component discriminant analysis: prioritizing relevant transcripts linked to the degradation of different carbohydrates in Pseudomonas putida S12. Microbiology. 2006, 152: 257-272. 10.1099/mic.0.28278-0.PubMedView ArticleGoogle Scholar
- Pieterse B, Jellema RH, van der Werf MJ: Quenching of microbial samples for increased reliability of microarray data. J Microbiol Methods. 2006, 64: 207-216. 10.1016/j.mimet.2005.04.035.PubMedView ArticleGoogle Scholar
- Ruijter GJG, Visser J: Determination of intermediary metabolites in Aspergillus niger. J Microbiol Methods. 1996, 25: 295-302. 10.1016/0167-7012(95)00104-2.View ArticleGoogle Scholar
- Koek M, Muilwijk B, van der Werf MJ, Hankemeier T: Microbial metabolomics with gas chromatography mass spectrometry. Anal Chem. 2006, 78: 1272-1281. 10.1021/ac051683+. [http://dx.doi.org/10.1021/ac051683+]PubMedView ArticleGoogle Scholar
- Verduyn C, Postma E, Scheffers WA, van Dijken JP: Physiology of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures. J Gen Microbiol. 1990, 136: 395-403.PubMedView ArticleGoogle Scholar
- Stein SE: An integrated method for spectrum extraction and compound identification from gas chromatography/mass spectrometry data. J Am Soc Mass Spectrom. 1999, 10: 770-781. 10.1016/S1044-0305(99)00047-1.View ArticleGoogle Scholar
- Mathworks: Matlab 7. 2005Google Scholar
- Eigenvector: PLS Toolbox 3.0. 2003Google Scholar
- Jansen JJ, Hoefsloot HCJ, Boelens HFM, van der Greef J, Smilde AK: Analysis of longitudinal metabolomics data. Bioinformatics. 2004, 20: 2438-2446. 10.1093/bioinformatics/bth268.PubMedView ArticleGoogle Scholar
- Box GEP, Cox DR: An Analysis of Transformations. J R Statist Soc B. 1964, 26: 211-252.Google Scholar
- Jolliffe IT: Springer Series in Statistics. Principal Component Analysis. 2002, New York, Springer-Verlag, Second EditionGoogle Scholar
- Krieger CJ, Zhang P, Mueller LA, Wang A, Paley S, Arnaud M, Pick J, Rhee SY, Karp PD: MetaCyc: a multiorganism database of metabolic pathways and enzymes. Nucleic Acids Res. 2004, 32: D438-D442. 10.1093/nar/gkh100.PubMedPubMed CentralView ArticleGoogle Scholar
- Kanehisa M, Goto S: KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 2000, 28: 27-30. 10.1093/nar/28.1.27.PubMedPubMed CentralView ArticleGoogle Scholar
- Efron B, Tibshirani RJ: An Introduction to the Bootstrap. 1993, New York, Chapman & Hall, 141-152.View ArticleGoogle Scholar
- Eisen MB, Spellman PT, Brown PO, Botstein D: Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci USA. 1998, 95: 14863-14868. 10.1073/pnas.95.25.14863.PubMedPubMed CentralView ArticleGoogle Scholar

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