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Table 2 Fluorescence ratio Kf determination by linear regression

From: Quantify single nucleotide polymorphism (SNP) ratio in pooled DNA based on normalized fluorescence real-time PCR

Num.

R0

Kf

 
 

Value.

SD

Value

SD

R2

Rep.1

0.0234

0.01

0.4111

0.0147

0.996175

Rep.2

0.0171

0.0119

0.4132

0.0157

0.995673

Rep.3

0.0132

0.0081

0.3595

0.0095

0.997902

Rep.4

-0.0161

0.0146

0.426

0.0184

0.994446

Kf Av.

  

0.40245a

0.014575b

 

Kf CV

7.30%c

    
  1. aAverage Kf value of four replicates
  2. bStandard Deviation of Kf value for four replicates
  3. cKf coefficient of variation
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BMC Genomics

ISSN: 1471-2164

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