Figure 4

Comparison of template specific hybridization pattern with QRT- PCR data. Signal intensities for matriptase, TIMP3 and PAI-1 were normalized to the ß-actin signal. Upper part: array data; lower part: QRT- PCR data. Cell lines: 97TM1, DLD-1, huFi (established line of human fibroblasts), primary synovial human fibroblasts either treated with IL-6 (huFiIL6) and TGFβ (huFiTGFß), respectively, for 24 h in FCS free medium, or non treated (huFiKo). Samples for the array experiments were labelled according to the linear amplification protocol with target specific primers. Amplifications in real time experiments were performed with gene specific primers (primer sequences: matriptase left primer: 5'-gat cct gca aaa ggg tga ga-3', right primer: 5'-cac ttt gga ggc tga gga ag-3'; TIMP3 left primer: 5'-ctt ctg caa ctc cga cat cg-3', right primer 5'-gta gtg ttt gga ctg gta gc-3'; PAI-1 left primer: 5'-gat cga ggt gaa cga gag t-3', right primer: 5'-cac agt gga ctc tga gat g-3').