Figure 3

Determination of optimal RT-PCR conditions for detection of both GSK-3β splice forms. On the left (A) GSK 3β expression is shown in dependence of various annealing temperatures. On the right (B) side GSK 3β expression is shown in dependence of the number of cycles. In figure C both annealing and extension times are varied. Abbreviations: H₂O, negative control; normal, normal GSK 3β isoform; alt, alternative splicing product.