| CTNNB1 | Ctbp1 (1) | Ctbp1 (2) | LRP 5 | ||||
---|---|---|---|---|---|---|---|---|
 | °C | min | °C | min | °C | min | °C | min |
activation | 94 | 3 | 94 | 3 | 94 | 3 | 94 | 3 |
Steps: | ||||||||
strand separation | 90 | 1,5 | 90 | 1,5 | 92 | 2 | 92 | 1,5 |
annealing | 66 | 1 | 58 | 1 | 59 | 1 | 64 | 1 |
elongation | 72 | 1 | 72 | 1,5 | 72 | 1,5 | 72 | 1,5 |
number of cycles | 33 | 30 | 30 | 30 | ||||
finale elongation | 72 | 10 | 72 | 10 | 72 | 10 | 72 | 10 |
 | GSK 3β | Axin-1 (A) | Axin-1 (B) |  |  | |||
 | °C | min | °C | min | °C | min |  |  |
activation | 94 | 3 | 94 | 3 | 94 | 3 | Â | Â |
Steps: | ||||||||
strand separation | 90 | 1,5 | 92 | 1,5 | 90 | 2 | Â | Â |
annealing | 63 | 1 | 63 | 1 | 65 | 1 | Â | Â |
elongation | 72 | 1 | 72 | 1,5 | 72 | 1,5 | Â | Â |
number of cycles | 30 | 30 | 30 | Â | Â | |||
finale elongation | 72 | 10 | 72 | 10 | 72 | 10 | Â | Â |