Table 1 Cloned and sequenced PR-10 sequences from B. pendula. Overview of the individual clones of PR-10 sequences from B. pendula 'Schneverdinger Goldbirke', 'Tristis', and 'Long Trunk'. Different primers (BpI, BpII) were used. De number of cycles varied between 22 and 30. Confirmed sequences are found in multiple independent PCRs. Unique sequences differ at least by three base pairs from any other sequence from the same cultivar. The remaining sequences are either recombination artifacts or presumably result from base mis-incorporations. The number of alleles included for further analysis is also indicated.

Primer combination

no. of PCR cycles

BpI

22 cycles

BpII

24 cycles

BpI

30 cycles

No. of different

alleles included

Confirmed sequences

43

(86%)

50

(69%)

24

(45%)

20

Unique sequences

2

(4%)

5

(7%)

5

(9%)

8

Recombination artifacts

1

(2%)

8

(11%)

18^*1

(34%)

Base mis-incorporation artifacts

4

(8%)

9

(13%)

6

(11%)

Total no. of clones

50

72

53

28

Cultivar 'Tristis'

Primer combination

no. of PCR cycles

BpI

22 cycles

BpII

24 cycles

BpI

30 cycles

No. of different

alleles included

Confirmed sequences

17

(77%)

20

(53%)

26

(54%)

11

Unique sequences

1

(5%)

5

(13%)

0

-

4

Recombination artifacts

2

(9%)

2

(5%)

22^*2

(46%)

Base mis-incorporation artifacts

2

(9%)

11

(29%)

0

-

Total no. of clones

22

38

48

15

Cultivar 'Schneverdinger Goldbirke'

Primer combination

no. of PCR cycles

BpI

26 cycles

BpII

26 cycles

BpI

30 cycles

No. of different

alleles included

Confirmed sequences

21

(53%)

13

(43%)

21

(43%)

10

Unique sequences

0

-

3

(10%)

3

(6%)

4

Recombination artifacts

12

(30%)

8

(27%)

15

(29%)

Base mis-incorporation artifacts

7

(18%)

6

(20%)

11

(22%)

Total no. of clones

40

30

51

14