Figure 5

Distribution of the fosmid-end sequences along the HBSQ001 chromosome showing coverage and discontinuities. Position for each fosmid-end is obtained from the best blast hit between the fosmid-end and the HBSQ001 chromosome. 'GC-HBSQ001' consists of the number of guanine (G) and cytosine (C) nucleotides out of every 10 nucleotide on the HBSQ001 chromosome (averaged over 10 Kb). At any corresponding nucleotide position on the HBSQ001 genome (x-axis): 'coverage' consists in the number (averaged over 10 Kb) of fosmids from the library where both ends have more than 80% DNA identity to HBSQ001 and for which distance between corresponding BLAST hit positions on the HBSQ001 genome is smaller than 60 Kb; discontinuities consist of the number (averaged over 60 Kb) of 1) fosmid end sequences with more than 80% DNA identity to HBSQ001 but for which the other end gives no hit to HBSQ001 and 2) fosmids for which distance between corresponding BLAST hit positions on the HBSQ001 chromosome is greater than 60 Kb. "Choppy" regions are highlited by a grey background.