From: Expression of target and reference genes in Daphnia magna exposed to ibuprofen
Relative expressiona | ||||||
---|---|---|---|---|---|---|
Gene | Control | 20 mg IB l-1 | 40 mg IB l-1 | 80 mg IB l-1 | Gene variabilityb | Amplification efficiency (E)a |
Act | 1.000 ± 0.078 | 0.963 ± 0.035 | 1.030 ± 0.044 | 1.001 ± 0.040 | 0.119 | 0.795 ± 0.066 |
Atb | 1.000 ± 0.129 | 0.896 ± 0.126 | 0.885 ± 0.095 | 0.613 ± 0.045 c | - | 1.010 ± 0.016 |
Cyp | 1.000 ± 0.040 | 0.949 ± 0.103 | 0.900 ± 0.015 | 0.817 ± 0.067 d | - | 1.038 ± 0.016 |
GAPDH | 1.000 ± 0.093 | 0.914 ± 0.059 | 0.919 ± 0.047 | 0.879 ± 0.067 | 0.098 | 0.946 ± 0.029 |
SDH | 1.000 ± 0.145 | 0.889 ± 0.170 | 0.950 ± 0.180 | 1.027 ± 0.026 | 0.160 | 0.992 ± 0.015 |
TBP | 1.000 ± 0.096 | 0.786 ± 0.176 | 0.788 ± 0.153 | 1.038 ± 0.084 | 0.192 | 0.925 ± 0.016 |
WARS | 1.000 ± 0.117 | 0.954 ± 0.098 | 0.906 ± 0.060 | 0.955 ± 0.081 | 0.136 | 0.922 ± 0.064 |
UBC | 1.000 ± 0.040 | 0.995 ± 0.095 | 1.024 ± 0.016 | 0.888 ± 0.097 | 0.098 | 1.034 ± 0.013 |
18S | 1.000 ± 0.279 | 2.096 ± 1.074 | 2.506 ± 1.640 | 2.204 ± 0.910 | 0.430 | 0.853 ± 0.089 |
28S | 1.000 ± 0.261 | 1.247 ± 0.445 | 1.410 ± 0.560 | 1.457 ± 0.616 | 0.313 | 0.846 ± 0.020 |