Table 2 Description of the primers used in this study to amplify the five exons of the chicken TYR gene, for the RACE reaction and the RT-PCR
Primers | Sequence 5' to 3' | Description |
|---|---|---|
exon1-up | TGGGCTTACTGCTGGTCATCC | Forward exon1 |
exon1-dwn | TGATGCTGGGCTGAGTAAATTAG | Reverse exon1 |
exon2-up | AATCTGCACTCAATCTGAAGAG | Forward exon2 |
exon2-dwn | TTCGGAAGCTGTAATTGGC | Reverse exon2 |
exon3-up | CCACACACTGCAATATCAAAC | Forward exon3 |
exon3-dwn | TCAACAAATGCATGGTGTAGG | Reverse exon3 |
exon4-up | CATTTTTGAGCGGTGGTTAAG | Forward exon4 |
exon4-dwn | ACTCATAGTCATACCCCAGC | Reverse exon4 |
exon5-up | ATCCCCTACCTCAAGCAAG | Forward exon5 |
exon5-dwn | CAGGAGTGTTGTTTAATCAAGC | Reverse exon5 |
PL1 | ATGCGCTGGCAGGTTCCTCTGTTGGAACGC | 5' RACE PCR1 |
PL2 | CAGCACTCCTTCCTCAGCAAGCTCTGCGTG | 5' RACE PCR2 |
P3'-1 | AGCGGTGGTTAAGAAGACACAGACC | 3' RACE PCR1 |
P3'-2 | GCTAGAAGTTTACCCAGCAGCCAAC | 3' RACE PCR2 |
3' UTR-RE* | AAACATATTGTCATTCTGCAGACA | 3'UTR primer sequence for the C*C |