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Table 2 Details of the sequenced fragments and polymorphisms indices found in analysed salmonid fish populations. Overlapping loci from the same gene have been combined as one index. Details of these SNPs have been included the sequences submitted to GenBank with the accession numbers [GenBank:DQ834848–DQ834885].

From: Single nucleotide polymorphism (SNP) discovery in duplicated genomes: intron-primed exon-crossing (IPEC) as a strategy for avoiding amplification of duplicated loci in Atlantic salmon (Salmo salar) and other salmonid fishes

  

Focal species

Locus

Source species a

Sequence length (bp)

No. individuals screened

Intronic/exonic region (bp)

No. SNPs and/or indels

Theta (x 104)

S. salar

      

U2A' (ii and iv)

Ssa

609

14

529/80

0

-

SS-II (i and ii)

Ssa

540

15

403/137

0

-

HMG-1 (ii)

Omy

505

15

475/30

0

-

TGF-beta (i and ii)

Omy

791

15

489/302

1

3.2

HMG-1 (iii)

Omy

540

15

394/146

0

-

Ran1 (iii)

Ssa

501

10

416/85

3

16.6

sGnRH (ii)

Ssa

562

15

481/81

0

-

GDF-8

Ssa

497

11

497/0

2

11.0

FGF6 (ii)

Omy

557

11

483/74

2

9.9

Vtg (ii)

Omy

447

15

277/170

0

-

ras-1(ii)

Omy

536

5

536/0

0

-

tnfa (ii)

Omy

522

13

462/60

0

-

IL-1 beta 2

Omy

399

10

399/0

1

7.1

RAG-1

Omy

563

15

563/0

0

-

c-myc

Omy

578

15

328/250

1

4.4

D(2)R

Omy

483

14

483/0

0

-

IgMh

Sal

518

15

518/0

2

9.7

rps24

Tru

776 c

11

unknown

5

17.5

CCBL1

Tru

298 d

4

unknown

0

-

epn b

Cca

359 e

5

unknown

0

-

epn(2) b

Cca

297 f

9

unknown

0

-

ssg(i)

Dre

470 g

15

unknown

1

5.4

EF1a

Dre

729 h

5

unknown

1

4.8

hox

Dre

834 h

9

unknown

0

-

Total

 

12 911

11.7 i

7 700/1 448 j

19

3.99

S. trutta

      

SS-II (ii)

Ssa

385

5

353/32

1

9.2

tap2A (ii)

Ssa

367

5

238/129

6 k

57.8

HMG-1 (iii)

Omy

540

4

394/146

0

-

TGF-beta (ii)

Omy

487

5

336/151

2

14.5

tnfa (ii)

Omy

514

4

454/60

6 l

45.0

Total

 

2293

4.6 i

1775/518

15

24.1

T. thymallus

      

SS-II (i)

Ssa

525

5

416/109

5

33.7

U2A' (ii)

Ssa

373

5

327/46

4

37.9

TGF-beta (i and ii)

Omy

743

5

441/302

3

14.3

RAG-1

Omy

376

5

376/0

2

18.8

Total

 

2017

5 i

1560/457

14

24.5

S. alpinus

      

SS-II (ii)

Ssa

354

5

322/32

0

-

HMG-1 (iii)

Omy

524

5

378/146

2

13.5

tnfa (ii)

Omy

512

5

452/60

0

-

total

 

1390

5 i

1152/238

2

5.1

  1. a – Cca, Cyprinus carpio; Dre, Danio rerio; Omy, Oncorhynchus mykiss; Sal, Salvelinus alpinus; Ssa, Salmo salar; Tru, Takifugu rubripes
  2. b – two separate PCR fragments extracted from the agarose gel were sequenced using the same primer pair
  3. c – 92% (346/376 bp) homology to S. salar zonadhesin-like gene, promoter region [GenBank:AY785950]
  4. d – 94% (228/242 bp) homology to O. mykiss multi tissue cDNA clone [GenBank:BX076795]
  5. e – 98% (195/198 bp) homology to S. salar white muscle cDNA library clone [GenBank:CK899537]
  6. f – 93% (230/245 bp) homology to S. salar mixed tissue cDNA clone [GenBank:CA055131]
  7. g – 95% (334/350 bp) homology to S. salar kidney cDNA library clone [GenBank:CK886988]
  8. h – no homologous sequences found after Blast search
  9. i – an average number of individuals screened over all loci
  10. j – the sum of intronic/exonic regions do not include 'unknown' sequences in S. salar
  11. k – three SNPs occurred as non-synonymous form in exonic regions
  12. l – one polymorphic site was located in synonymous site of exonic region