Figure 6

GFP-fused LmjF28.1470 chimeras localise to different cellular compartments. (A) Top; schematic of the structure from the GFP-fused LmjF28.1470. Bottom; localisation of GFP-fused LmjF28.1470 in live L. major promastigotes (DIC image shown on the top-left). The GFP fluorescence signal is shown in green and the flagellar pocket (Fp), labelled by FM4-64 at 4°C is shown in red. The merged signals, together with the DAPI staining of DNA (blue; n: nucleus; k: kinetoplast) are shown in the enlarged image. Co-localised signal is shown in yellow. Scale bar = 10 μm. (B) Top; schematic of the structure from the GFP-fused short version of LmjF28.1470. Middle; localisation of GFP-fused short LmjF28.1470 in live L. major promastigotes (inset shows DIC image). The GFP fluorescence signal is shown in green merged together with the DAPI staining of DNA in blue (n: nucleus; k: kinetoplast). Bottom; localisation of GFP-fused short LmjF28.1470 in fixed L. major promastigotes. Insets show the DIC image, the GFP fluorescence signal in green and immuno-labelling with ER marker LCB2 in red. The merged fluorescent signals, together with the DAPI staining of DNA (blue; n: nucleus; k: kinetoplast) are shown on the enlarged image. Co-localised signal is shown in yellow. Scale bar = 10 μm.