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Table 2 Oligonucleotide primers. Oligonucleotide primers used in PCR assays to clone different segments of the toxin-antitoxin system from pGI1.

From: TasA-tasB, a new putative toxin-antitoxin (TA) system from Bacillus thuringiensis pGI1 plasmid is a widely distributed composite mazE-doc TA system

Segment

Region amplified (Accession number : AY138809)a

Oligonucleotide primersb

Primer names

Length (bp) of amplified region

A

(304–629)C

5'-TTAGGCATATG ACAGCAAACACTCGCCACATGG-3'

DOTF

345 bp

  

5'-AATATGTCGAC TCATTTCGGTTTTCCTACTTTCCTCC-3'

DOTR2

 

P

(8147–8254,1–332)C

5'-GGCCCGGTACC GTGGAGGAAAGTAGGAAAACCG-3'

pOIf

459 bp

  

5'-CCCGTCGAC CCCACGTCTTTTTTTAATTTAAAGG-3'

pOIr

 

AP

(8147–8254,1–629)C

5'-TTAGGCATATG ACAGCAAACACTCGCCACATGG-3'

DOTF

754 bp

  

5'-CCCGTCGAC CCCACGTCTTTTTTTAATTTAAAGG-3'

pOIr

 

pAP

(8147–8254,1–861)C

5'-AACGGTTAATACGTTCTAACTG-3'

ValC

978 bp

  

5'-CCCGTCGAC CCCACGTCTTTTTTTAATTTAAAGG-3'

pOIr

 

p2AP

(8147–8254,1–939)C

5'-GGCTCTAGAATCTTCCCTATC-3'

ValC2

1056 bp

  

5'-CCCGTCGAC CCCACGTCTTTTTTTAATTTAAAGG-3'

pOIr

 

RTA

(370...630)C

5'-AATGACAGCAAACACTCGCC-3'

RTAf

261 bp

  

5'-GCACCGTCAAATTTATTGATGG-3'

RTAr

 

RTP

(8249–8254,1–228)C

5'-GAGGTGTTAAAGATACAGG-3'

RTPf

234 bp

  

5'-AATCTGAAGCTTCTTGTTCGG-3'

RTPr

 

RTAP

(8249–8254,1–630)C

5'-AATGACAGCAAACACTCGCC-3'

RTAf

636 bp

  

5'-AATCTGAAGCTTCTTGTTCGG-3'

RTPr

 
  1. a: C indicates complementary sequences.
  2. b: Letters in bold refer to restriction sites.
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BMC Genomics

ISSN: 1471-2164

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