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Figure 5 | BMC Genomics

Figure 5

From: Hyaluronan- and RNA-binding deubiquitinating enzymes of USP17 family members associated with cell viability

Figure 5

Deubiquitinating enzyme assays for novel USP17 subfamily members. In vitro deubiquitinating enzyme activity for USP17K, USP17L, USP17M, and USP17N. Immunoblotting analysis was performed with anti-β-gal antiserum. Coexpressed plasmids were pGEX-2TK (lane 1), pGEX-2TK-USP17K (lane 2), pGEX-2TK-USP17K (C89S) (lane 3), pGEX-2TK-USP17L (lane 4), pGEX-2TK-USP17L (C89S) (lane 5), pGEX-2TK-USP17M (lane 6), pGEX-2TK-USP17M (C89S) (lane 7), pGEX-2TK-USP17N (lane 8), and pGEX-2TK-USP17N (C89S) (lane 9). All of normal USP17 subfamily members cleaved the linkage between ubiquitin and β-galactosidase.

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BMC Genomics

ISSN: 1471-2164

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