Figure 6

Correlation between ANILFR (Average Normalized Inter-Locus Fluorescence Ratio) value identified by array-CGH and copy number determined by mpFISH. X-axis: copy number of chr3 regions (R1, R2...) identified by interphase and/or metaphase mpFISH (see "Copy nr FISH" in table 1). Y-axis: ANILFR value for the same regions (see ANILFR in Table 1). As example for UOK125 cell line: Region R1, has an ANILFR of 1.07 (Y-axis value), which corresponds to 2 copies of this region on chr3 (X- axis value). (see Figure 5C and Table 1). These (x, y) coordinates define the first data point (pink romb) for the UOK125 trend line. The second data point corresponds to R2 with ANILFR of 1.46 (Y-axis value), and with a copy number of 3 (X- axis value). Each cell line has a number of data points (rombs of respective colour) corresponding to the number of chr3 region of different copy number (Table 1 – Region). Trend lines for each cell line were calculated and drawn. The interception of trend lines on Y-axis indicates the intensity of the background fluorescence "B" (corresponding to 0 copies, homozygous deletion region in U2020, ANILFR of 0.28). The slope of the trend lines reflects ploidy level. At the right from the graph, the ploidy number obtained from chromosome counts is shown for the respective cell lines ("Ploidy C" on Table 1). See other explanations in the text.