Figure 1
From: Identification of rat lung – prominent genes by a parallel DNA microarray hybridization
Reproducibility of hybridizations. (A-C):typical scatter plots of self-self hybridization of lung cDNAs between two channels within a block (within-block, panel A), two different blocks in one slide (within-slide, panel B), and among slides (among-slide, panel C), respectively. The cDNAs from an identical lung tissue were labeled with Cy3 or Alexa 647, and hybridized to each block of the slides. The numbers on x- and y-axis were background-subtracted fluorescence intensities of each spot with log2 transformation. (D) A comparison of correlation coefficients from replicated hybridizations. The results were expressed as means ± SE. *P < 0.01 v.s. among-slide; #P < 0.01 v.s. within-slide. (E) Comparison of accumulated errors between within-slide and among-slide groups. For the within-slide group, the log ratios were from parallel hybridization on a single slide. For the among-slides, the log ratios were from different slides. The accumulated errors were calculated as described in Materials and Methods.