Table 1 Summary of the selected SNPs and the respective method of genotyping. Gene/SNP, rs number given when available, aminoacid change or UTR. Primer set: for the assyas developed for this study or otherwise as referred to original publication. Method of analysis: platform and assay selection.
From: Multilocus analysis of SNP and metabolic data within a given pathway
Gene/SNP | Primer set | Method of analysis |
|---|---|---|
CYP11A1, repeat | f(5'-6-FAM-GTC-AGC-TGT-ACT-GAA-TTA-CAG-CC-3') r(5'-TCT-TGC-CAG-CTT-GGG-CAA-CAT-ACG-3' | Fragment analysis ABI310, (Applied Biosystems) |
CYP17, rs 743572 | f 5'-CAT-TCG-CAC-CTC-TGG-AGT-3' r 5'-GGC-TCT-TGG-GGT-ACT-TG-3' | RLFP Msp A1 (Promega), |
CYP19 3'UTRSNP1, rs10046 | 5'-ATA TTC TGG CAA CTG TCT-3' 5'-GAG GAT GAC ACT ATT GGC-3' | BDT (Big Dye Terminator) sequencing ABI310 (Applied Biosystems) |
CYP19 3'UTRSNP2, rs4646 | 5'-GAC AGT GTG TTG AGA GCA TAC AGA-3' 5'-TTC TTC ACC GAC TAT TTC TC-3' | BDT (Big Dye Terminator) sequencing ABI310 (Applied Biosystems) |
CYP19 3'UTRSNP3, rs2255192 | 5'-CAT TGA ATC ATT GTA TGT GGT CAT-3' 5'-GGA AAT GGG ATG GAA ATA GAT TAC A-3' | BDT (Big Dye Terminator) sequencing ABI310 (Applied Biosystems) |
HSD-A3T, rs3138620 | 5'-6-FAM CAG TAC TAA AGG CCC TAT TAT CAA A-3' 5'-AGG CTG CAG TGA GTC CAG AT-3' | Fragment analysis ABI310, (Applied Biosystems) |
HSD-DEL, rs8191194 | 5'-6-FAM GTG ACC CAC GAA ACA CAG G-3' 5'-CAG AAG GTG AAG AAC TCA TCC A-3' | Fragment analysis on a ABI310, (Applied Biosystems) |
CYP1B1 R48G | 5'-GCACCCCTGAGTGTCACGCC-3', 5'-TTCCAGTGCTCCGAGTAGTG-3' | RFLP, Rsr II. (Fermentas) |
CYP1B1 A119S | 5'-GCACCCCTGAGTGTCACGCC-3', 5'-TTCCAGTGCTCCGAGTAGTG-3' | RLFP, NgoM IV (New England BioLabs) |
CYP1B1 V432L, rs1056836 | 5'-CCAGCCCAACCTGCCCTATG-3' 5'-CCAGGATGGAGATGAAGA-3' | RLFP, Eco577 (Fermentas) |
CYP1A1m1 T3801C | Cascorbi et [19]. | See reference |
CYP1A1m2 I462V, rs1048943 | Cascorbi et [19]. | See reference |
CYP1A1mS T3205C | Cascorbi et [19]. | See reference |
CYP1A1m4 T461N, rs1799814 | Cascorbi et [19]. | See reference |
COMT, rs4680 | Matsui et al. 2000, [20]. | See reference |
GSTM1 | Kristensen et al 1998 [31]. | See reference |
GSTT1 | Kristensen et al 1998[31]. | See reference |
GSTP1 | Kristensen et al 1998[31]. | See reference |