Figure 4

Phylogenetic tree analysis of Mde8i18-5 orthologs. The dendrogram was constructed using the sequences that fit the criteria for ortholog determination. There were 2 members of this gene family in the Anopheles and Drosophila genome (ENSANGP00000022276 and ENSANGP00000000768, and CG2987 and CG17947 respectively). CG2987 had 2 associated transcripts (RA and RB). Human orthologs of the proteins (CTNNAL1 and CTNNAL2) were included to verify orthology predictions. The A. gambiae white gene is the outgroup. Md8i18_5 was most similar to the predicted Anopheles ortholog, and then to the Drosophila ortholog followed by the human ortholog. The second member of this gene family grouped separately verifying that the most likely Anopheles and Drosophila genes.