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Figure 2 | BMC Genomics

Figure 2

From: A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination

Figure 2

Sequence features of a typical read. Sequence was generated from the negative strand with the M13 Reverse primer (M13R). Exact matches to the wild-type and lox 71 and lox 66 inverted repeat mutants were required for spacers to proceed to further analysis. The NotI site, pUC19 vector boundary and EcoRI were also detectable. The HindIII site could not be detected with the M13 Reverse primer.

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BMC Genomics

ISSN: 1471-2164

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