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Table 3 Total reactions and mutations per position in the 8 bp loxP spacer region organized by mismatch Type

From: A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination

Type

Pos 1

Pos 2

Pos 3

Pos 4

Pos 5

Pos 6

Pos 7

Pos 8

TotalReactions

Type I

0

0

0

0

0

0

0

0

47

Type II

209

0

0

0

0

0

0

244

303

Type III

0

144

126

1

0

167

143

0

284

Type IV

1,575

1,361

1,104

3

1

1,349

1,331

2,128

2,490

Total

1,784

1,505

1,230

4

1

1,516

1,474

2,372

3,124