Figure 1

Comparative genomic analysis of SPRN. (A) Gene order and relative gene orientations in the local SPRN genomic contexts located on the human chr. 10 (Hs), mouse chr. 7 (Mm), gray short-tailed opossum chr. 1 (Md), chicken chr. 6 (Gg), western clawed frog scaffold_502 (Xt), Japanese medaka chr. 15 (Ol) and three-spine stickleback chr. 6 (Ga). Detailed genomic sequence coordinates were given in section 4.1. Gene names were explained in the main text. Genes were drawn approximately to scale. The horizontal bar shows 10 kb sequence length. (B) Conserved region in SPRN promoters. Sequence coordinates were calculated relative to introns. Horizontal lines denote predicted Sp1 sites in human (above alignment) and chicken (below alignment). (C) Conserved region in SPRN introns. Sequence coordinates were calculated relative to exon 2. CCC, C-rich intron splicing enhancer sequence; CTCTCT, polypyrimidine tract-binding protein-binding site sequence; AG, 3' intron splice site sequence. (D) Conserved motifs in the conserved SPRN 3'-UTR region 7. Sequence coordinates were calculated relative to ORFs where possible. miRNA, potential hsa-miR-34a site (CACTGCCA). (B-D) White letters on black background indicate conservation in 100% sequences, white letters on dark gray background indicate conservation in ≥75% sequences and black letters on light gray background indicate conservation in ≥50% sequences. Ga, Gasterosteus aculeatus; Gg, Gallus gallus; Hs, Homo sapiens; Md, Monodelphis domestica; Mm, Mus musculus; Ol, Oryzias latipes; Xt, Xenopus tropicalis.