Figure 4

Phenotype of the rgh *-00S-005-14. (A) Self-pollinated ear segregating for rgh kernels (arrows). (B) Mature normal and rgh sibling kernels. The top row shows the abgerminal side of the kernels, and the bottom row shows the germinal side of the kernels. Scale bars are 5 mm. (C) Longitudinal hand sections of normal and rgh kernels. Aleurone, starchy endosperm, and embryo tissues are denoted by a, s, and e, respectively. Arrows indicate embryonic shoot and root tissues in the normal kernel. Scale bars are 1 mm. (D) Normal and rgh mutant plants 50 days after initiating tissue culture. (E) Homozygous rgh mutant ear at 35 DAP. (F) Native PAGE assay for 6PGDH activity from 25 DAP endosperm extracts. Black arrow indicates the inferred PGD3 isozyme and the open arrow indicates the PGD1 and PGD2 homo- and heterodimer isozymes.