Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways
© Swindell et al; licensee BioMed Central Ltd. 2007
Received: 16 October 2006
Accepted: 22 May 2007
Published: 22 May 2007
The heat shock response of Arabidopsis thaliana is dependent upon a complex regulatory network involving twenty-one known transcription factors and four heat shock protein families. It is known that heat shock proteins (Hsps) and transcription factors (Hsfs) are involved in cellular response to various forms of stress besides heat. However, the role of Hsps and Hsfs under cold and non-thermal stress conditions is not well understood, and it is unclear which types of stress interact least and most strongly with Hsp and Hsf response pathways. To address this issue, we have analyzed transcriptional response profiles of Arabidopsis Hsfs and Hsps to a range of abiotic and biotic stress treatments (heat, cold, osmotic stress, salt, drought, genotoxic stress, ultraviolet light, oxidative stress, wounding, and pathogen infection) in both above and below-ground plant tissues.
All stress treatments interact with Hsf and Hsp response pathways to varying extents, suggesting considerable cross-talk between heat and non-heat stress regulatory networks. In general, Hsf and Hsp expression was strongly induced by heat, cold, salt, and osmotic stress, while other types of stress exhibited family or tissue-specific response patterns. With respect to the Hsp20 protein family, for instance, large expression responses occurred under all types of stress, with striking similarity among expression response profiles. Several genes belonging to the Hsp20, Hsp70 and Hsp100 families were specifically upregulated twelve hours after wounding in root tissue, and exhibited a parallel expression response pattern during recovery from heat stress. Among all Hsf and Hsp families, large expression responses occurred under ultraviolet-B light stress in aerial tissue (shoots) but not subterranean tissue (roots).
Our findings show that Hsf and Hsp family member genes represent an interaction point between multiple stress response pathways, and therefore warrant functional analysis under conditions apart from heat shock treatment. In addition, our analysis revealed several family and tissue-specific heat shock gene expression patterns that have not been previously described. These results have implications regarding the molecular basis of cross-tolerance in plant species, and raise new questions to be pursued in future experimental studies of the Arabidopsis heat shock response network.
The heat shock response network of Arabidopsis thaliana involves temperature perception mechanisms, an intricate array of signal transduction networks, and twenty-one known transcription factors that activate heat shock proteins and other heat-stress related genes [1–3]. The availability of genome sequence data has considerably advanced our understanding of this heat shock response pathway, as well as the molecular basis of regulatory networks that underlie other forms of environmental stress in Arabidopsis (e.g., cold, salinity, drought). One result of this development has been increased recognition of the cross-talk or overlap that exists among cellular responses to different environmental stress treatments [4–8]. In this respect, heat shock proteins (and their associated transcription factors) are of special interest. Heat shock proteins are molecular chaperones that regulate the folding, localization, accumulation, and degradation of protein molecules in both plant and animal species . Heat shock proteins are thus believed to play a broad role in many cellular processes, which may impart a generalized role in tolerance to multiple environmental stress treatments apart from heat stress. Understanding the role of heat shock proteins under cold and non-thermal stress conditions may therefore provide insight into multiple stress tolerance mechanisms . This may be of considerable importance for improving the production of agriculturally important crop species under field conditions, which are best characterized as an interaction of several different types of stress, rather than just a single stress treatment in isolation .
The Arabidopsis heat shock proteins (Hsps) and transcription factors (Hsfs) have been well characterized on the basis of genome sequence information [1, 11–14]. In addition to the twenty-one known transcription factors , the Arabidopsis heat shock response is partly mediated by thirteen Hsp20 proteins , eighteen Hsp70 proteins , seven Hsp90 proteins , and up to eight members of the Hsp100 protein family . The molecular pathways leading to Hsp expression are not entirely understood , but involve temperature perception mechanisms coupled with multiple signal transduction pathways , which together lead to the activation of Hsfs that induce expression of heat shock genes by binding to heat shock elements . There are several levels at which this molecular pathway may overlap with those underlying response to cold and non-thermal stress treatments. However, since Hsps play a uniquely broad role in cellular processes, Hsps are particularly likely to underlie interactions between heat and non-heat stress response pathways. A role of Hsps in cellular response to cold and non-heat stress treatments, for instance, has been supported by several gene expression studies. In Arabidopsis and other plant species, various Hsps have been induced by low temperature , osmotic stress , salt , oxidative stress [19–22], desiccation , exposure to intense light [24, 25], wounding , and heavy metal exposure .
While a number of studies have shown that Hsp expression can be induced under cold and non-thermal stress treatments, no comparative analysis has been carried out to identify which particular stress treatments are the weakest and strongest inducers of Hsp expression. It therefore remains unclear which stress-response pathways overlap most extensively with this important part of the Arabidopsis heat shock regulatory network. If the primary stress conditions interacting with Hsp response pathways can be identified, it would be of considerable interest to understand how Hsfs and Hsps contribute to tolerance under such stress conditions. The physiological role of Hsfs and Hsps in promoting tolerance may differ depending on the nature of the stress imposed upon the cell. Heat stress, for instance, leads directly to denaturation of cellular proteins. It is therefore clear how molecular chaperone activity may contribute to high temperature tolerance via prevention of deleterious protein conformations and elimination of non-native aggregations. With respect to cold and non-thermal stress treatments, however, the impact on cellular protein conformations is less direct and not as well understood. The role of Hsps as molecular chaperones, therefore, may not strictly parallel their function under heat stress, and it is possible that their cellular function extends beyond the chaperone activity that has been well characterized in vitro [27, 28]. One possibility, for example, is that Hsps limit damage resulting from accumulation of reactive oxygen species, which are generated as messengers and elements of signal transduction pathways under a wide range of stress conditions . In both plant and animal species, for instance, there is evidence to suggest that Hsps protect against reactive oxygen species [30–38]. This hypothesis is particularly intriguing in light of the considerable interconnectivity that exists between heat shock and oxidative stress response pathways in plant species [21, 22, 39, 40].
DNA microarray technology offers a promising approach for better understanding the functional role of Arabidopsis heat shock proteins and transcription factors under both heat and non-heat stress conditions. Recently, a number of genome-wide microarray datasets have been generated and made publicly available by the AtGenExpress consortium . These resources provide an opportunity to profile Hsf and Hsp expression over a wide range of stress conditions simultaneously. In this study, we utilized AtGenExpress datasets to analyze transcriptional responses of Arabidopsis Hsfs and Hsps to a total of ten different abiotic and biotic stress treatments (cold, osmotic stress, salt, drought, genotoxic stress, ultraviolet light, oxidative stress, wounding, high temperature, and pathogen infection). For all abiotic stress treatments, we analyzed expression measurements generated from both below (root) and above-ground (shoots) tissue samples, while with respect to pathogen infection treatment, expression measurements generated from leaf tissue were considered. In all treatments, expression measurements obtained at up to six different time points of stress exposure (0.5, 1, 3, 6, 12, and 24 hours). With respect to each of five protein families (Hsf, Hsp20, Hsp70, Hsp90, and Hsp100), we evaluated whether expression responses of each family to each stress were significantly large in comparison to other Arabidopsis genes. This analysis provided indication of which types of stress interacted most and least with each protein family. In addition, we characterized Hsf and Hsp stress-response patterns at the level of protein families, as well as among individual genes within protein families. This allowed identification of family-level expression patterns under each stress, gene sub-groups within families exhibiting similar expression patterns, and individual Hsf/Hsp genes with large expression responses to multiple stress treatments.
Overview: Responsiveness of heat shock gene families to abiotic and biotic stress treatments.
Hsf (n = 21)
Hsp20 (n = 18)
Hsp70 (n = 13)
Hsp90 (n = 6)
Hsp100 (n = 7)
Protein families exhibiting strong expression responses to many stress treatments exhibit a generalized expression response pattern. The Hsf and Hsp20 were associated with the most stress-general expression patterns, since for both families, T was significant for nearly all types of stress (see Table 1). In contrast, the Hsp70, Hsp90, and Hsp100 families were not so widely responsive across stress treatments. Expression response patterns associated with each protein family are described in the following sections. Two additional files related to these results are available online. Additional file 1 contains summary results from differential expression analyses, heat maps of clustering solutions, and raw signal intensities of Hsf and Hsp genes (present/absent calls). Additional file 2 contains expression response profiles for each individual Hsf and Hsp gene under the stress conditions we examined.
Heat shock transcription factors
Members of the heat shock transcription factor protein family
Cluster ID (Development)
Cluster ID (Stress)
Hsp20 protein family
Members of the Hsp20 protein family
Cluster ID (Development)
Cluster ID (Stress)
Hsp70, Hsp90, and Hsp100 protein families
Members of the Hsp70 protein family
Cluster ID (Development)
Cluster ID (Stress)
Members of the Hsp90 protein family
Cluster ID (Development)
Cluster ID (Stress)
Members of the Hsp100 protein family
Cluster ID (Development)
Cluster ID (Stress)
Clustering of Hsp90 genes with respect to stress-response patterns assigned five members to one group (AtHsp90-2, 4, 5, 6, and 7), since these genes were all associated with highly similar (and weak) expression response patterns in root tissue (see section 4C of additional file 1). The remaining AtHsp90-1 exhibited a strong expression response pattern distinct from all other AtHsp90 genes, and was therefore assigned to a singleton cluster (see Table 5). Within Hsp70 and Hsp100 families, clustering with respect to stress-response patterns identified few sub-groups among member genes (see Tables 4 and 6).
Various members of the Hsp70, Hsp90, and Hsp100 families were associated with the same developmental expression pattern found among certain Hsf and Hsp20 genes. This pattern was best exhibited by AtHsp70-4, AtHsp70-11, AtHsp90-1, and AtHsp100-1, all of which were highly expressed in roots (17 days), flowers stage 12, flowers stage 15, and seed stages 6 – 10 (see sections 3C, 4C, and 5C of additional file 1).
Heat shock proteins (Hsps) and transcription factors (Hsfs) are central components of the Arabidopsis thaliana heat shock regulatory network. It has long been recognized that these elements are also involved in response to cold and non-thermal stress treatments , but the types of stress that most strongly elicit Hsp/Hsf expression responses have not been identified, and the physiological role of these proteins under non-heat stress treatments is unclear. The findings of this study support the hypothesis that Hsps and Hsfs represent an intersection point between heat and non-heat stress response pathways. Our results indicate that, to varying extents, each of nine cold and non-thermal stress treatments interact with Hsfs and Hsps at the level of gene expression. Several prominent family-level expression response patterns were identified. These included highly similar stress-response profiles among Hsp20 proteins, a number of Hsps specifically upregulated 12 hours after wounding and during recovery following heat stress, and upregulation of heat shock genes to UV-B stress in shoot but not root tissue. Our findings raise important questions to be pursued in future experimental studies of the Arabidopsis heat shock response network.
Genome-wide transcriptional profiling allowed the expression of Hsf and Hsp genes under many stress conditions to be examined within the same context. This facilitated identification of which stressors interact with each protein family most strongly, which provides insight into the nature and degree of cross-talk that exists between heat and other forms of stress. The osmotic, cold, and salt treatments were among the strongest inducers of heat shock gene expression. These stress treatments induced expression responses of heat shock genes that were large in comparison to other Arabidopsis genes (see Table 1), and also large in an absolute sense, since these stressors induced strong fold-changes and differential expression of individual Hsf and Hsp genes (see Figs. 1, 2, 3, 4 and additional files). Expression response patterns were very similar under each of these treatments, with upregulation primarily occurring over the late stages of stress exposure (3 – 24 hours). Since osmotic, cold, and salt stress treatments are each believed to have a deleterious impact on cellular water potential [42, 43], it is possible that their impact on heat shock genes is related to this common effect. In support of this notion, several previous studies in plant species have implicated Hsp20 proteins in tolerance to water stress treatments [17, 44, 45]. Among other stress treatments, wounding and UV-B stress induced moderately large expression responses of heat shock genes (with strong differences among families and between tissue types). The pathogen infection treatment was unique, since in contrast to other types of stress, it elicited strong expression responses among the Hsp70, Hsp90, and Hsp100 families, while most members of the Hsf and Hsp20 family were not responsive. Overall, drought and genotoxic stress treatments were associated with weak induction of heat shock genes, although some individual genes can be cited as an exception to this trend (e.g., HsfA8, AtHsp15.4-CI(r), AtHsp100-7).
The degree to which oxidative stress impacted heat shock gene expression is difficult to discern. In comparison to other Arabidopsis genes, all protein families (except Hsp90) exhibited large expression responses to oxidative stress (see Table 1). However, since genomic expression responses to oxidative stress were generally small, absolute fold-changes induced by oxidative stress were nonetheless of small magnitude. Only one transcription factor, for instance, was differentially expressed under oxidative stress (HsfA1d). These results were surprising, since there is considerable evidence that heat shock transcription factors can function as reactive oxygen species sensors in plants , and extensive interactions have been identified between heat and oxidative stress molecular pathways [33, 38, 39, 46, 47]. Moreover, since the generation of reactive oxygen species is a general response under many types of stress , Hsf activation by reactive oxygen species may provide the best hypothesis to explain why heat shock genes are induced by so many stress treatments. In view of this, an important factor to consider is the means by which oxidative stress was experimentally induced. For data we analyzed, oxidative stress was induced by exogenous application of methyl viologen, which is a generator of superoxide anion radical . The impact of this reactive oxygen species on heat shock gene expression may differ from that of others [49, 50]. In a recent study, for instance, Gadjev et al. (2006)  demonstrated that among genes upregulated more than two-fold under heat stress, relatively few were responsive to superoxide anion radical, while most were instead responsive to the singlet oxygen reactive oxygen species. These considerations suggest that, although the oxidative stress treatment examined by this study may not have had a strong impact on heat shock genes, the production of different types of reactive oxygen species (e.g., H2O2), leading to Hsf activation and consequently Hsp expression, remains a pathway through which cellular responses to heat and other forms of stress may be linked.
Heat shock transcription factors are of fundamental importance to understanding stress response networks, since these proteins coordinate the expression of Hsps and other stress-responsive genes. The Arabidopsis Hsf family is larger than that which has been described in any animal system , and at present, no single Hsf has been identified as a primary trigger of the heat shock response. The emerging picture is one of considerable complexity, with extensive interactions among individual Hsfs and sensitivity to a diverse range of environmental signals . We found that seven Hsfs (six class A, one class B) exhibited very weak expression responses to heat and all other stress conditions (see Table 2, stress-clusters 451 and 452), while the remaining 14 Hsfs were strongly induced by several stress treatments. The most distinctive expression response patterns we observed were associated with HsfA6b and HsfC1 (see additional file 2). In root tissue, HsfA6b exhibited approximately five-fold induction to salt and osmotic treatments across all time points of gene expression measurement (0.5 – 24 hours). This pattern contrasted with that observed among other Hsfs, most of which responded to salt and osmotic stress over the late stages of stress exposure only. This early response of HsfA6b to salt and cold treatments was, in fact, unique among all the heat shock genes that we examined, suggesting that HsfA6b may interact with elements outside of the Hsf/Hsp response pathway. On the basis of differential expression analysis, HsfC1 was the most stress-responsive of all Hsfs. Among all treatments and tissues that we examined, this transcription factor was, on average, differentially expressed with respect to nearly half of the time points at which gene expression was measured. This strong expression response pattern is particularly noteworthy in light of the large structural dissimilarities between HsfC1 and all other Arabidopsis Hsfs .
The Hsp20 family exhibited the most stress-general expression response pattern of all the protein groups that we examined. Our results therefore suggest that this protein family is of potential importance as a factor contributing to multiple stress tolerance in plant species. These findings are also consistent with those of previous studies, which have found that certain Hsp20 proteins are involved in cellular responses to a wide variety of environmental treatments besides heat, such as alcohol , cold , heavy metals [52–55], osmotic stress , desiccation , and oxidative stress . At present, little is known regarding how Hsp20 proteins are integrated with molecular networks that underlie cellular responses to these stress treatments. Increasingly, it has been recognized that Hsp20 proteins can engage in a wide range of cellular processes under stress, including ATP-independent stabilization of substrate proteins undergoing conformational disruption , or associating with lipid molecules to regulate fluidity of the membrane structure . This latter function suggests that Hsp20s could be involved in the perception of stressful stimuli leading to the activation of signal transduction pathways. Under temperature extremes, the role of membrane fluidity as a means of stress perception and activation of signal transduction pathways has been well established . However, since non-thermal stressors may also alter membrane fluidity or lead to various types of membrane damage, interactions of Hsp20s with membranes could partly account for the overall stress-responsiveness of the Hsp20 family.
A striking aspect of the Hsp20 family was the similarity among the expression response patterns of member genes. This similarity was demonstrated by our clustering analysis (see section 2C of additional file 1), which interestingly, revealed a cluster of five 17 kDa Hsp20 proteins that included both class I and II nuclear/cytosolic proteins. This result is consistent with findings of previous studies, which have identified functional similarities between class I and II Hsp20s , despite marked differences between the amino acid sequences of the two classes . If analysis is restricted to stress responses occurring in the root tissue type only, the overall homology of Hsp20 expression response patterns is considerably enhanced. In root tissue, expression patterns of 17 kDa Hsp20s are very similar to those of the 18 – 20 kDa Hsp20s, including those localized to the mitochondria and endoplasmic reticulum (see section 2C of additional file 1). The similarity of expression patterns among the Hsp20 proteins may reflect shared induction mechanisms, and possibly extensive coordination among Hsp20s as cellular chaperones, such as that observed during the formation of heat-stress granules . Shared induction mechanisms among Hsp20 proteins may include accumulation of denatured proteins in the cytoplasm , generation of reactive oxygen species , or changes in membrane lipid composition and fluidity . These processes are thought to be upstream signals leading to the activation of critical Hsfs, which are most likely the direct inducers of Hsp20 expression under stress.
A number of Hsps were upregulated 12 hours after wounding, with a parallel expression response pattern during recovery from heat stress in root tissue. While the majority of these proteins were members of the Hsp20 family (see Fig. 5), some members of the Hsp70, Hsp90, and Hsp100 families also exhibited this distinctive expression pattern (see Fig. 7). The upregulation of multiple Hsps following wounding and during heat stress recovery has not been previously documented in Arabidopsis or other plant species, and is therefore an important finding of this study. The first indication that Hsps are involved in the wounding response pathway was provided by the study of Cheong et al. (2002) , in which the effect of wounding on expression levels of 8,200 Arabidopsis genes was surveyed in leaf tissue. Cheong et al. (2002)  identified one Hsf upregulated 0.5 hours following wounding (AtHsfA4a), along with another upregulated both 0.5 and 6 hours after wounding (AtHsfB1). In addition, several Hsp70 proteins were upregulated 6 hours after wounding, as well as two 17 kDa sHSPs (AtHsp17.8-CII and AtHsp17.7-CII). In our study, the most interesting wounding-response patterns occurred in root tissue, but our results are consistent with those of , since Hsp upregulation also occurred after wounding in aerial shoot tissue. Overall, our findings suggest that Hsp involvement in wounding response is greater than previously recognized, and by profiling Hsps simultaneously under multiple types of stress, our results show that late wound-responsive genes are also expressed during recovery from heat stress. These results point to a broad role of some Hsps during stress recovery or acclimation. Following wounding of plant tissue, both local and systemic signals are generated that coordinate defense responses aimed at limiting further injury (e.g., by pathogen) . Similarly, following exposure to high temperatures, plants exhibit increased tolerance or hardening to limit damage resulting from future temperature elevations . The functional role of Hsps upregulated as part of this post-wounding and post-heat stress response is unclear and warrants further investigation. With respect to heat stress recovery, one recent study found that mutant plants lacking a 32 kDa heat shock associated protein (Hsa32) exhibited an elevated decay in thermotolerance following exposure to heat stress .
Ultraviolet-B radiation resulted in upregulation of heat shock proteins and transcription factors in shoots, but did not have this effect in root tissue. This distinction between aerial and subterranean tissue types was most marked with respect to the Hsp20 group, in which nearly all Hsp20s were upregulated in shoots and downregulated in roots. Similar to other stress treatments, exposure to ultraviolet-B light has been associated with the production of reactive oxygen species [63, 64]. Specifically, ultraviolet light stress has been found to increase cellular concentrations of H2O2 , which has been thought to activate Hsf expression , especially that of HsfA4a and HsfA8 . We found that both HsfA4a and HsfA8 were strongly induced by UV-B stress in shoots but not in roots (see additional file 2). These observations are consistent with the notion that Hsp expression in shoots results from UV-B induced activation of Hsfs, possibly HsfA4a and HsfA8, with the generation of H2O2 as an intermediary signal. Given the tissue-specific effect we observed, however, the generation of H2O2 could be dependent upon interactions between UV-B stress and photosynthetic processes taking place in chloroplast. In previous models, it has been suggested that UV-B generated reactive oxygen species are upstream components that act upon photosynthetic genes (i.e., H2O2 → photosynthesis) [66, 67]. Our results, however, suggest that the reverse is also plausible, in which photosynthetic processes are upstream components leading to the generation of reactive oxygen species under UV-B stress (i.e., photosynthesis → H2O2).
It has recently been emphasized that the generation of agricultural varieties tolerant to a range of stress conditions should be a primary goal in biotechnological applications, since under field conditions, plants may encounter different types of stress in combination . Focusing on overlapping elements among response pathways that underlie diverse forms of stress may advance our knowledge of cross-tolerance in plant species . The Arabidopsis heat shock proteins and transcription factors exhibit expression responses under a wide range of stressful stimuli, and are therefore a natural model for developing our understanding of integration between regulatory networks associated with different kinds of stress. The findings of this study have identified which types of stress interact least and most strongly with Hsfs and each Hsp family at the transcriptional level. In addition, new family and tissue-specific expression response patterns have been uncovered. These patterns include concerted expression response profiles among Hsp20 proteins, specific upregulation of most Hsp20 proteins (some Hsp70, Hsp90, and Hsp100 proteins) twelve hours following wounding of root tissue, and with respect to all heat shock gene families, upregulation under ultraviolet-B light stress in aerial (shoots) but not subterranean tissue (roots). These results provide insight into the nature and degree of cross talk between heat and non-heat stress conditions, and represent a basis for further experimental investigations into the involvement of Hsf and Hsp proteins under cold and non-thermal stress.
All microarray data analyzed in this study were generated using the ATH1 Affymetrix microarray platform [69, 70], with expression estimates obtained by gcRMA normalization . A total of 22,810 probes were included on the ATH1 platform, along with 64 control probes not corresponding to Arabidopsis genes. Our analysis is therefore based on a total of 22,746 genes, representing approximately 80% of all known Arabidopsis genes . Abiotic stress and pathogen series expression datasets were downloaded from AtGenExpress . Complete protocols associated with these data can be obtained from TAIR (submission numbers: ME00325, ME00326, ME00327, ME00328, ME00329, ME00330, ME00338, ME00339, ME00340, ME00342) . In brief, the abiotic stress series data consists of gene expression measurements performed on Arabidopsis thaliana (col-0) roots and shoots under a benign control condition and nine environmental stress conditions. Root samples consisted of all below-ground plant tissue, while shoot samples consisted of all above-ground green tissues (including leaves). For each stress condition, expression measurements were obtained from 16 to 18-day old plants at six different time points of stress-exposure (1/2, 1, 3, 6, 12, and 24 hours). All expression measurements were performed with duplicate biological replications and no technical replications. Stress treatments included cold (4°C), osmotic stress (300 mM Mannitol), salt (150 mM NaCl), drought (15 min. dry air stream leading to 10% loss of fresh weight), genotoxic stress (1.5 μg/ml bleomycin, 22 μg/ml mitomycin), oxidative stress (10 μM methyl viologen), ultraviolet-B light stress (15 min. exposure, 1.18 W/m2 Phillips TL40W/12), wounding (pin puncture), and high temperature (3 hrs. at 38°C followed by 21 hrs. recovery at 25°C). From the pathogen series dataset, we considered experiments involving P. infestans infection of 5-week old Arabidopsis leaves, along with corresponding control treatments in which H2O was applied to leaves. Only the treated leaf tissue was used in RNA extractions and microarray analyses (excluding all other green tissues). Expression measurements were obtained at each of three post-infection time points (6, 12, and 24 hours), with three biological replications at each time (no technical replication). Pathogen infections used 10-8 cfu/ml in MgCl2 with 5 × 105 P. infestans spores applied to leaf surfaces.
Heat shock proteins and transcription factors
The heat shock proteins and transcription factors analyzed in this study were selected based upon the genomic sequence analyses performed by [1, 11–14]. Our analysis includes all of the twenty-one Hsfs identified by . Several Hsps identified by the above-cited studies were not represented on the ATH1 array (AtHsp17.8-Cl, AtHsp70-12, AtHsp70-13, AtHsp70-14, AtHsp70-16, AtHsp70-18, AtHsp90-3, and AtHsp100-6), and therefore could not be included in this study. In total, our heat shock protein analysis is based upon 18 of 19 members of the Hsp20 family (12 sHsps and 6 related sHsp-like proteins), 13 of 17 members of the Hsp70 family (11 DnaK and 2 SSE subfamily), 6 of 7 members of the Hsp90 family, and 7 of 8 members of the Hsp100 family (AtHsp100-1 and six homologues). The expression response patterns of each Hsf and Hsp gene were analyzed with respect to nine abiotic stress treatments (applied to root and shoot tissue), in addition to pathogen infection treatment (applied to leaf tissue). In total, therefore, the expression response of each Hsf and Hsp was examined under 19 tissue-treatment combinations.
The magnitude of T reflects how large expression responses of a protein family are, on average, with respect to a given tissue-treatment combination. The significance of observed T statistics was evaluated under the null hypothesis that the n genes in each protein family are a random sample of the N = 22746 genes represented on the ATH1 array, versus the alternative that the n genes are a non-random sample yielding a T statistic larger than expected within a random sample. This hypothesis was evaluated by the following resampling procedure. With respect to each tissue-treatment combination and each protein family, a total of 103 random samples of n genes were drawn from among all N = 22746 genes, and the value of T was calculated from each of the 103 random samples. This yielded null distributions specific to each tissue-treatment combination and protein family, which were used to evaluate the significance of observed T statistics. An observed T statistic was significant if the proportion of random samples yielding a larger or equal T statistic was less than α = 0.05. A significant T statistic indicates that the expression responses among the n members of a protein family (with respect to a given tissue-treatment combination) are larger than expected within a random sample of n genes.
Hsf and Hsp expression response patterns within protein families and among individual genes were analyzed by differential expression analysis and clustering . Differential expression analysis was carried out using the Limma linear modeling package available in the R Bioconductor software suite . In this approach, a linear model was fit for all genes with respect to each of the 19 tissue-treatment combinations. This allowed heat shock related genes to be tested for differential expression at every time point associated with each tissue-treatment combination. For each of the 19 linear model analyses performed, P-values were adjusted for multiple comparisons using the Benjamini and Hochberg method [77, 78]. The differential expression analysis was used to construct the index (d) introduced in the Results section.
The hierarchical ordered partitioning and collapsing hybrid (HOPACH) clustering algorithm was used to identify sub-groups of genes with similar expression response patterns in each protein family . In this algorithm, the number of clusters appropriate in the final clustering solution is determined automatically according the median split silhouette criterion . The HOPACH algorithm is particularly well-suited for finding homogenous clusters of small size among a limited number of genes. Stress-clusters were formed by grouping Hsf/Hsp genes with respect to their expression responses (M) under all 111 tissue-treatment-time combinations included in our analysis (18 tissue-treatment combinations with 6 time points + 1 tissue-treatment combination with 3 time points). The Euclidean distance metric was used to measure similarity between vectors of expression responses (M) associated with each Hsf/Hsp gene. To form developmental-clusters, genes were centered to have a mean expression intensity of zero across the 79 developmental series conditions, and the cosine angle similarity metric was used to cluster expression profiles of Hsf/Hsp genes within each family.
This work was supported by National Science Foundation award MCB-0348074a and a research grant from the Michigan State University Quantitative Biology and Modeling Initiative. Additional support was provided by the Department of Probability and Statistics and Department of Plant Biology at Michigan State University. The authors gratefully acknowledge members of the AtGenExpress consortium (Thomas Altmann, Pascal von Koskull-Döring, Jörg Kudla, Lutz Nover, Detlef Weigel) and the Arabidopsis Functional Genomics Network for providing the gene expression data analyzed in this study. In addition, we thank two anonymous reviewers for their helpful comments on this manuscript.
- Nover L, Bharti K, Döring P, Mishra SK, Ganguli A, Scharf K-D: Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?. Cell Stress Chaperones. 2001, 6: 177-189.PubMed CentralPubMedView ArticleGoogle Scholar
- Sung DY, Kaplan F, Lee K-J, Guy GL: Acquired tolerance to temperature extremes. Trends Plant Sci. 2003, 8: 179-187.PubMedView ArticleGoogle Scholar
- Larkindale J, Hall JD, Knight MR, Vierling E: Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol. 2005, 138: 882-897.PubMed CentralPubMedView ArticleGoogle Scholar
- Cheong Y, Chang H, Gupta R, Wang X, Zhu T, Luan S: Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal response in Arabidopsis. Plant Physiol. 2002, 129: 661-677.PubMed CentralPubMedView ArticleGoogle Scholar
- Rensink WA, Lobst S, Hart A, Stegalkina S, Liu J, Buell CR: Gene expression profiling of potato responses to cold, heat, and salt stress. Funct Integ Genom. 2005, 5: 201-207.View ArticleGoogle Scholar
- Ma S, Gong Q, Bohnert HJ: Dissecting salt stress pathways. J Exp Bot. 2006, 57: 1097-1107.PubMedView ArticleGoogle Scholar
- Mittler R: Abiotic stress, the field environment and stress combination. Trends Plant Sci. 2006, 11: 15-19.PubMedView ArticleGoogle Scholar
- Rossel JB, Walter PB, Hendrickson L, Chow WS, Poole A, Mullineaux PM, Pogson BJ: A mutation affecting ascorbate peroxidase 2 gene expression reveals a link between responses to high light and drought tolerance. Plant Cell Environ. 2006, 29: 269-281.PubMedView ArticleGoogle Scholar
- Feder ME, Hofmann GE: Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annu Rev Physiol. 1999, 61: 243-282.PubMedView ArticleGoogle Scholar
- Hoffmann AA, Parsons PA: Evolutionary genetics and environmental stress. 1991, Oxford: Oxford University PressGoogle Scholar
- Scharf K-D, Siddique M, Vierling E: The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing α-crystallin domains (Acd proteins). Cell Stress Chaperones. 2001, 6: 225-237.PubMed CentralPubMedView ArticleGoogle Scholar
- Lin B-L, Wang J-S, Liu H-C, Chen R-W, Meyer Y, Barakat A, Delseny M: Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana. Cell Stress Chaperones. 2001, 6: 201-208.PubMed CentralPubMedView ArticleGoogle Scholar
- Krishna P, Gloor G: The Hsp90 family of proteins in Arabidopsis thaliana. Cell Stress Chaperones. 2001, 6: 238-246.PubMed CentralPubMedView ArticleGoogle Scholar
- Agarwal M, Katiyar-Agarwal S, Sahi C, Gallie DR, Grover A: Arabidopsis thaliana Hsp100 proteins: kith and kin. Cell Stress Chaperones. 2001, 6: 219-224.PubMed CentralPubMedView ArticleGoogle Scholar
- Schöffl F, Prandl R, Reindl A: Regulation of the heat-shock response. Plant Physiol. 1998, 117: 1135-1141.PubMed CentralPubMedView ArticleGoogle Scholar
- Sabehat A, Lurie S, Weiss D: Expression of small heat-shock proteins at low temperatures – A possible role in protecting against chilling injuries. Plant Physiol. 1998, 117: 651-658.PubMed CentralPubMedView ArticleGoogle Scholar
- Sun WN, Bernard C, van de Cotte B, Van Montagu M, Verbruggen N: At-Hsp17.6A, encoding a small heat-shock protein in Arabidopsis, can enhance osmotolerance upon overexpression. Plant J. 2001, 27: 407-415.PubMedView ArticleGoogle Scholar
- Liu NY, Ko SS, Yeh KC, Charng YY: Isolation and characterization of tomato Hsa32 encoding a novel heat-shock protein. Plant Science. 2006, 170: 976-985.View ArticleGoogle Scholar
- Banzet N, Richaud C, Deveaux Y, Kazmaier M, Gagnon J, Triantaphylides C: Accumulation of small heat shock proteins, including mitochondrial Hsp22, induced by oxidative stress and adaptive response in tomato cells. Plant J. 1998, 13: 519-527.PubMedView ArticleGoogle Scholar
- Lee BH, Won SH, Lee HS, Miyao M, Chung WI, Kim IJ, Jo J: Expression of the chloroplast-localized small heat shock protein by oxidative stress in rice. Gene. 2000, 245: 283-290.PubMedView ArticleGoogle Scholar
- Desikan R, A-H-Mackerness S, Hancock JT, Neill SJ: Regulation of the Arabidopsis transcriptome by oxidative stress. Plant Physiol. 2001, 127: 159-172.PubMed CentralPubMedView ArticleGoogle Scholar
- Volkov RA, Panchuk II, Mullineaux PM, Schöffl F: Heat stress-induced H2O2 is required for effective expression of heat shock genes in Arabidopsis. Plant Mol Biol. 2006, 61: 733-746.PubMedView ArticleGoogle Scholar
- Liu D, Zhang X, Cheng Y, Takano T, Liu S: rHsp90 gene expression in response to several environmental stresses in rice (Oryza sativa L.). Plant Physiol Biochem. 2006, 44: 380-386.PubMedView ArticleGoogle Scholar
- Hihara Y, Kamei A, Kanehisa M, Kaplan A, Ikeuchi M: DNA microarray analysis of cyanobacterial gene expression during acclimation to high light. Plant Cell. 2001, 13: 793-806.PubMed CentralPubMedView ArticleGoogle Scholar
- Rossel JB, Wilson IW, Pogson BJ: Global changes in gene expression in response to high light in Arabidopsis. Plant Physiol. 2002, 130: 1109-1120.PubMed CentralPubMedView ArticleGoogle Scholar
- Györgyey J, Gartner A, Nemeth K, Magyar Z, Hirt H, Heberlebors E, Dudits D: Alfalfa heat shock genes are differentially expressed during somatic embryogenesis. Plant Mol Biol. 1991, 16: 999-1007.PubMedView ArticleGoogle Scholar
- Lee GJ, Roseman AM, Saibil HR, Vierling E: A small heat shock protein stably binds heat-denatured model substrates and can maintain a substrate in a folding-competent state. EMBO J. 1997, 16: 659-671.PubMed CentralPubMedView ArticleGoogle Scholar
- Lee GJ, Vierling E: A small heat shock protein cooperates with heat shock protein 70 systems to reactivate a heat-denatured protein. Plant Physiol. 2000, 122: 189-197.PubMed CentralPubMedView ArticleGoogle Scholar
- Pastori GM, Foyer H: Common components, networks, and pathways of cross-tolerance to stress. The central role of "redox" and abscisic acid-mediated controls. Plant Physiol. 2002, 129: 460-468.PubMed CentralPubMedView ArticleGoogle Scholar
- Mehlen P, Briolay J, Smith L, Diazlatoud C, Fabre N, Pauli D, Arrigo AP: Analysis of the resistance to heat and hydrogen peroxide stresses in cos cells transiently expressing wild-type or deletion mutants of the Drosophila 27-kda heat-shock protein. Eur J Biochem. 1993, 215: 277-284.PubMedView ArticleGoogle Scholar
- Fleming JE, Reveillaud I, Niedzwiecki A: Role of oxidative stress in Drosophila aging. Mutat Res. 1992, 275: 267-279.PubMedView ArticleGoogle Scholar
- Wheeler JC, Bieschke ET, Tower J: Muscle-specific expression of Drosophila hsp70 in response to aging and oxidative stress. Proc Natl Acad Sci. 1995, 92: 10408-10412.PubMed CentralPubMedView ArticleGoogle Scholar
- Härndahl U, Hall RB, Osteryoung KW, Vierling E, Bornman JF, Sundby C: The chloroplast small heat shock protein undergoes oxidation-dependent conformational changes and may protect plants from oxidative stress. Cell Stress Chaperones. 1999, 4: 129-138.PubMed CentralPubMedView ArticleGoogle Scholar
- Preville X, Salvemini F, Giraud S, Chaufour S, Paul C, Stepien G, Ursini MV, Arrigo AP: Mammalian small stress proteins protect against oxidative stress through their ability to increase glucose-6-phosphate dehydrogenase activity and by maintaining optimal cellular detoxifying machinery. Exp Cell Res. 1999, 247: 61-78.PubMedView ArticleGoogle Scholar
- Ding QX, Keller JN: Proteasome inhibition in oxidative stress neurotoxicity: implications for heat shock proteins. J Neurochem. 2001, 77: 1010-1017.PubMedView ArticleGoogle Scholar
- Martindale JL, Holbrook NJ: Cellular response to oxidative stress: signaling for suicide and survival. J Cell Physiol. 2002, 192: 1-15.PubMedView ArticleGoogle Scholar
- Kregel KC: Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. J Appl Physiol. 2002, 92: 2177-2186.PubMedView ArticleGoogle Scholar
- Neta-Sharir I, Isaacson T, Lurie S, Weiss D: Dual role for tomato heat shock protein 21: protecting photosystem II from oxidative stress and promoting color changes during fruit maturation. Plant Cell. 2005, 17: 1829-1838.PubMed CentralPubMedView ArticleGoogle Scholar
- Panchuk II, Volkov RA, Schöffl F: Heat stress and heat shock transcription factor dependent expression and activity of ascorbate peroxidase in Arabidopsis. Plant Physiol. 2002, 129: 838-853.PubMed CentralPubMedView ArticleGoogle Scholar
- Miller G, Mittler R: Could heat shock transcription factors function as hydrogen peroxide sensors in plants?. Ann Bot. 2006, 98: 279-288.PubMed CentralPubMedView ArticleGoogle Scholar
- Schmid M, Davison TS, Henz SR, Pape UJ, Demar M, Vingron M, Scholkopf B, Weigel D, Lohmann JU: A gene expression map of Arabidopsis thaliana development. Nat Genet. 2005, 37: 501-506.PubMedView ArticleGoogle Scholar
- Kreps JA, Wu Y, Hur-Song C, Zhu T, Wang X, Harper JF: Transcriptomic changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol. 2002, 130: 2129-2141.PubMed CentralPubMedView ArticleGoogle Scholar
- Verslues PE, Agarwal M, Katiyar-Agarway S, Zhu JH, Zhu JK: Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J. 2006, 45: 523-539.PubMedView ArticleGoogle Scholar
- Almoguera C, Coca MA, Jordano J: Tissue-specific expression of sunflower heat-shock proteins in response to water-stress. Plant J. 1993, 4: 947-958.View ArticleGoogle Scholar
- Coca MA, Almoguera C, Thomas TL, Jordano J: Differential regulation of small heat-shock genes in plants: analysis of a water-stress-inducible and developmentally activated sunflower promoter. Plant Mol Biol. 1996, 31: 863-876.PubMedView ArticleGoogle Scholar
- Pnueli L, Liang H, Rozenberg M, Mittler R: Growth suspension, altered stomatal responses, and augmented induction of heat shock proteins in cytosolic ascorbate peroxidase (Apx1)-deficient Arabidopsis plants. Plant J. 2003, 34: 187-203.PubMedView ArticleGoogle Scholar
- Davletova S, Schlauch K, Coutu J, Mittler R: The zinc-finger protein Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis. Plant Physiol. 2005, 139: 847-856.PubMed CentralPubMedView ArticleGoogle Scholar
- Laloi C, Apel K, Danon A: Reactive oxygen signaling: the latest news. Curr Opin Plant Biol. 2004, 7: 323-328.PubMedView ArticleGoogle Scholar
- op den Camp RGL, Przybyla D, Ochsenbein C, Laloi C, Kim C, Danon A, Wagner D, Hideg E, Göbel C, Feussner I, Nater M, Apel K: Rapid induction of distinct stress responses after release of singlet oxygen in Arabidopsis. Plant Cell. 2003, 15: 2320-2332.PubMed CentralPubMedView ArticleGoogle Scholar
- Gadjev I, Vanderauwera S, Gechev TS, Laloi C, Minkov IN, Shulaey V, Apel K, Inze D, Mittler R, Van Breusegem F: Transcriptomic footprints disclose specificity of reactive oxygen species signaling in Arabidopsis. Plant Physiol. 2006, 141: 436-445.PubMed CentralPubMedView ArticleGoogle Scholar
- Kuo HF, Tsai YF, Young LS, Lin CY: Ethanol treatment triggers a heat shock-like response but no thermotolerance in soybean (Glycine max cv. Kaohsiung No.8) seedlings. Plant Cell Environ. 2000, 23: 1099-1108.View ArticleGoogle Scholar
- Lin CY, Roberts JK, Key JL: Acquisition of thermotolerance in soybean seedlings: synthesis and accumulation of heat shock proteins and their cellular localization. Plant Physiol. 1984, 74: 152-160.PubMed CentralPubMedView ArticleGoogle Scholar
- Tseng TS, Tzeng SS, Yeh KW, Yeh CH, Chang FC, Chen YM, Lin CY: The heat-shock response in rice seedlings – isolation and expression of cDNAs that encode class-I low-molecular-weight heat-shock protein. Plant Cell Physiol. 1993, 34: 165-168.Google Scholar
- Guan JC, Jinn TL, Yeh CH, Feng SP, Chen YM, Lin CY: Characterization of the genomic structures and selective expression profiles of nine class I small heat shock protein genes clustered on two chromosomes in rice (Oryza sativa L.). Plant Mol Biol. 2004, 56: 795-809.PubMedView ArticleGoogle Scholar
- Sun WN, Van Montagu M, Verbruggen N: Small heat shock proteins and stress tolerance in plants. Gene Structure and Expression. 2002, 1577: 1-9.View ArticleGoogle Scholar
- Wehmeyer N, Vierling E: The expression of small heat shock proteins in seeds responds to discrete developmental signals and suggests a general protective role in desiccation tolerance. Plant Physiol. 2000, 122: 1099-1108.PubMed CentralPubMedView ArticleGoogle Scholar
- Tsvetkova NM, Horvath I, Torok Z, Wolkers WF, Balogi Z, Shigapova N, Crowe LM, Tablin F, Vierling E, Crowe JH, Vigh L: Small heat-shock proteins regulate membrane lipid polymorphism. Proc Natl Acad Sci. 2002, 99: 13504-13509.PubMed CentralPubMedView ArticleGoogle Scholar
- Löw D, Brändle K, Nover L, Forreiter C: Cytosolic heat-stress proteins Hsp17.7 class I and Hsp17.3 class II of tomato act as molecular chaperones in vivo. Planta. 2000, 211: 575-582.PubMedView ArticleGoogle Scholar
- Vierling E: The roles of heat-shock proteins in plants. Annu Rev Plant Phys Mol Biol. 1991, 42: 579-620.View ArticleGoogle Scholar
- Nover L, Scharf KD, Neumann D: Formation of cytoplasmic heat-shock granules in tomato cell-cultures and leaves. Mol Cell Biol. 1983, 3: 1648-1655.PubMed CentralPubMedView ArticleGoogle Scholar
- Leon J, Rojo E, Sanchez-Serrano JJ: Wound signaling in plants. J Exp Bot. 2001, 52: 1-9.PubMedView ArticleGoogle Scholar
- Charng YY, Liu HC, Liu NY, Hsu FC, Ko SS: Arabidopsis Hsa32, a novel heat shock protein, is essential for acquired thermotolerance during long recovery after acclimation. Plant Physiol. 2006, 140: 1297-1305.PubMed CentralPubMedView ArticleGoogle Scholar
- Arnott T, Murphy TM: A comparison of the effects of a fungal elicitor and ultraviolet-radiation on ion-transport and hydrogen-peroxide synthesis by rose cells. Env Exp Bot. 1991, 31: 209-216.View ArticleGoogle Scholar
- Green R, Fluhr R: UV-B-induced PR-1 accumulation is mediated by active oxygen species. Plant Cell. 1995, 7: 203-212.PubMed CentralPubMedView ArticleGoogle Scholar
- Shiu CT, Lee TM: Ultraviolet-B-induced oxidative stress and responses of the ascorbate-glutathione cycle in a marine macroalga Ulva fasciata. J Exp Bot. 2005, 56: 2851-2865.PubMedView ArticleGoogle Scholar
- A-H-Mackerness S, Surplus SL, Blake P, John CF, Buchanan-Wollaston V, Jordan BR, Thomas B: Ultraviolet-B-induced stress and changes in gene expression in Arabidopsis thaliana : role of signaling pathways controlled by jasmonic acid, ethylene and reactive oxygen species. Plant Cell Environ. 1999, 22: 1413-1423.View ArticleGoogle Scholar
- John CF, Morris K, Jordan BR, Thomas B, A-H-Mackerness S: Ultraviolet-B exposure leads to up-regulation of senescence-associated genes in Arabidopsis thaliana. J Exp Bot. 2001, 52: 1367-1373.PubMedView ArticleGoogle Scholar
- Bowler C, Fluhr R: The role of calcium and activated oxygens as signals for controlling cross-tolerance. Trends Plant Sci. 2000, 5: 241-246.PubMedView ArticleGoogle Scholar
- Hennig L, Menges M, Murray JA, Gruissen W: Arabidopsis transcript profiling on Affymetrix GeneChip arrays. Plant Mol Biol. 2003, 53: 457-465.PubMedView ArticleGoogle Scholar
- Redman JC, Haas BJ, Tanimoto G, Town CD: Development and evaluation of an Arabidopsis whole genome Affymetrix probe array. Plant J. 2004, 38: 545-561.PubMedView ArticleGoogle Scholar
- Wu Z, Irizarry R, Gentleman R, Martinez Murillo F, Spencer F: A model based background adjustment for oligonucleotide expression arrays. J Amer Stat. 2004, 99: 909-917.View ArticleGoogle Scholar
- AtGenExpress. [http://www.weigelworld.org/resources/microarray/AtGenExpress/]
- The Arabidopsis Information Resource. [http://www.arabidopsis.org/]
- Swindell WR, Huebner M, Weber AP: Plastic and adaptive gene expression patterns associated with temperature stress in Arabidopsis thaliana. Heredity.
- Nettleton D: A discussion of statistical methods for design and analysis of microarray experiments for plant scientists. Plant Cell. 2006, 18: 2112-2121.PubMed CentralPubMedView ArticleGoogle Scholar
- Smyth GK: Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol. 2004, 3: Article 3.Google Scholar
- Benjamini Y, Hochberg Y: Controlling the false discovery rate: a powerful and practical approach to multiple testing. J Roy Stat Soc B. 1995, 57: 289-300.Google Scholar
- Reiner A, Yekutieli D, Benjamini Y: Identifying differentially expressed genes using false discovery rate controlling procedures. Bioinformatics. 2003, 19: 368-375.PubMedView ArticleGoogle Scholar
- van der Lann M, Pollard K: Hybrid clustering of gene expression data with visualization and bootstrap. J Stat Plan Inference. 2003, 117: 275-303.View ArticleGoogle Scholar
- Pollard K, van der Laan M: Cluster analysis of genomic data. Bioinformatics and Computational Biology Solutions Using R and Bioconductor. Edited by: Gentleman R, Huber W, Carey VJ, Irizarry RA, Dudoit S. 2005, New York: Springer, 209-228.View ArticleGoogle Scholar
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