Figure 3

gRNA identification method and validation. A) A local alignment was performed for each minicircle sequence against a library of predicted edited mRNA sequences yielding a single best score. B) 1000 permutations and local alignment batteries were performed as a method of calculating the approximate probability of a given best score. C) The best hybridization scores for each minicircle sequence were ranked by cumulative probability from 0 to 1 (circles). All points to the left of the intersection with the false discovery rate threshold (heavy solid line) were deemed to represent scores from predicted gRNAs. D) Using false discovery rate to control for multiple testing, alignments with scores deemed significant were said to be predicted gRNAs.