Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici)

Ling, Peng; Wang, Meinan; Chen, Xianming; Campbell, Kimberly Garland

doi:10.1186/1471-2164-8-145

Table 2 cDNA clones showing homology to genes with characterized or unclassified proteins through Blastx search of the NCBI fungal databases

From: Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici)

Category & clone no.	GenBank accession	Size (bp)	Full length or partial^a	Best hit in the NCBI databases
				Protein	Accession	Organism	e-value
1. Amino acid metabolism
35I14	EG374455	766	F	Cystathionine beta-lyase	gi\|6636350	Botryotinia fuckeliana	5.70E+00
2. Cell Defense
66C24a	EG374440	1175	P	88 kDa immunoreactive mannoprotein MP88	gb\|AAL87197.1	Cryptococcus neoformans	1.00E-03
3. Cell Division/cycle
10F19	EG374412	1877	F	g1/s-specific cyclin pcl1 (cyclin hcs26)	gb\|AAW44590.1	Cryptococcus neoformans	2.00E-04
4. Cell signaling/cell communication
65G15	EG374514	1106	P	Protein kinase	gi\|15072451	Cryphonectria parasitica	1.20E+00
30E21	EG374336	1128	F	Serine/threonine kinase	gi\|22531808	Ustilago maydis	3.90E-01
65C6	EG374367	1649	F	Serine/threonine phosphatase	gi\|33087517	Hypocrea jecorina	3.90E-01
80G5b	EG374428	1230	P	Mitogen-activated protein kinase	gi\|57227328	Cryptococcus neoformans	1.70E-00
5. Cell Structure and growth
58G9	EG374486	1714	F	Beta tubulin	gi\|47834278	Penicillium flavigenum	6.40E-00
40G6b	EG374274	888	P	Cell wall protein	gi\|68471254	Candida albicans	4.60E-01
58C4b	EG374296	819	P	Cell surface protein	gi\|70983232	Aspergillus fumigatus	2.60E-02
10D19	EG374322	1212	F	Cell wall mannoprotein	ref\|NP_012685.1	Saccharomyces cerevisiae	1.00E-03
90I19	EG374406	1240	F	Cell wall mannoprotein	gi\|6322611	Saccharomyces cerevisiae	1.50E-02
90C22	EG374402	1641	F	Cytoplasm protein	gb\|AAW42379.1	Cryptococcus neoformans	1.00E-04
10I15	EG374326	1088	F	Mitochondrial outer membrane beta-barrel protein	gi\|45758780	Neurospora crassa	1.70E-01
60H1	EG374354	1035	F	Nuclear pore complex subunit	gi\|46437749	Candida albicans	5.00E-00
70I19a	EG374443	1132	P	Nucleoskeletal-like protein	gi\|172053	Saccharomyces cerevisiae	1.30E-01
6. Differentiation- related protein
70A18	EG374371	1207	F	Differentiation-related protein	gb\|AAD38996.1	Uromyces appendiculatus	6.00E-03
7. Mating type
30M10	EG374346	1025	F	Mating type alpha locus	gi\|73914085	Cryptococcus gattii	6.80E+00
30C22	EG374334	1110	F	Mating type alpha locus	gi\|73914085	Cryptococcus gattii	7.50E+00
8. Nucleotide metabolism
35K8	EG374456	1572	F	Ribonuclease H2 subunit	gi\|6320485	Saccharomyces cerevisiae	9.00E+00
9. Protein translational modification
100C10	EG374490	1179	F	Non-ribosomal peptide synthetase	gi\|62006079	Hypocrea virens	1.20E+00
10. Ribosomal protein complex
35L17	EG374457	585	F	18S ribosomal RNA	gi\|51102377	Microbotryum dianthorum	4.20E-02
40C19a	EG374512	706	P	18S ribosomal RNA	gi\|28412377	Leotiomycete sp.	5.40E-01
35H2b	EG374500	786	P	26S large subunit ribosomal RNA	gi\|30313824	Pichia guilliermondii AjvM13	1.00E-03
35E4	EG374451	897	F	28S ribosomal RNA	gi\|46810582	Fuscoporia viticola	5.00E-03
35P11a	EG374506	667	P	28S ribosomal RNA	gi\|62005826	Puccinia artemisiae-keiskeanae	1.00E-04
55B15	EG374473	954	F	28S ribosomal RNA	gi\|84794517	Puccinia striiformoides	3.60E-01
58B3	EG374484	884	F	28S ribosomal RNA	gi\|46810582	Fuscoporia viticola	3.30E-01
58N22	EG374488	996	F	28S ribosomal RNA	gi\|20452324	Rhodotorula pilati	3.30E-01
66I12	EG374338	1167	F	28S ribosomal RNA	gi\|46810582	Fuscoporia viticola	3.00E-04
80G5a	EG374427	1106	P	Calnexin	gi\|45551624	Aspergillus fumigatus	2.30E-00
11. Sugar/glycolysis metabolism
58G18b	EG374304	796	P	Pyruvate decarboxylase	gi\|68480982	Candida albicans	1.40E+00
10N6	EG374330	1029	F	Pyruvate kinase	gi\|168073	Aspergillus nidulans	6.00E+00
12. Transport metabolism
30G15	EG374339	1087	F	Membrane zinc transporter	gi\|47156070	Aspergillus fumigatus	5.70E-01
40H8a	EG374275	656	P	amino acid transporter	gi\|70985369	Aspergillus fumigatus	3.10E+00
80K19	EG374395	1728	F	Na+-ATPase	gi\|1777377	Zygosaccharomyces rouxii	2.00E-04
55L18b	EG374289	845	P	Peptide transporter	gi\|70982509	Aspergillus fumigatus	5.30E-01
13. Unclassified
80G10	EG374391	1132	F	Genomic sequence	gi\|48056381	Phakopsora pachyrhizi	7.00E-53
04F9	EG374470	1127	F	Hypothetical protein	gi\|71006713	Ustilago maydis	1.00E-06
10N10	EG374331	1106	F	Hypothetical protein	gi\|58258450	Cryptococcus neoformans	6.00E-22
30I21	EG374342	1906	F	Hypothetical protein	gi\|71023234	Ustilago maydis	1.00E-21
35B6	EG374449	1060	F	Hypothetical protein	gb\|EAA67250.1	Gibberella zeae	1.00E-03
35C10	EG374450	1465	F	Hypothetical protein	gi\|71004383	Ustilago maydis 521	2.00E-08
35G21	EG374454	1332	F	Hypothetical protein	gb\|EAK81105.1	Ustilago maydis	5.00E-09
35H2a	EG374499	758	P	Hypothetical protein	gi\|71021872	Ustilago maydis	1.80E+00
40B2a	EG374508	603	P	Hypothetical protein	gi\|85114517	Neurospora crassa	3.00E-05
40C12a	EG374510	792	P	Hypothetical protein	gi\|71019552	Ustilago maydis	4.00E-01
55L8	EG374491	1417	F	Hypothetical protein	gi\|71004813	Ustilago maydis	1.50E-01
58C4a	EG374296	764	P	Hypothetical protein	MGG_09875.5^b	Magnaporthe grisea	6.00E-12
60D4	EG374350	1123	F	Hypothetical protein	gi\|50259357	Cryptococcus neoformans	7.00E-04
60I14	EG374356	1565	F	Hypothetical protein	gi\|58263159	Cryptococcus neoformans	2.00E-09
60L15	EG374359	2073	F	Hypothetical protein	gb\|EAA47832.1	Magnaporthe grisea	7.00E-10
60N2	EG374363	1109	F	Hypothetical protein	gi\|46096746	Ustilago maydis	7.00E-03
60N6	EG374364	1071	F	Hypothetical protein	gi\|49642978	Kluyveromyces lactis	8.00E-17
65H5	EG374374	1390	F	Hypothetical protein	gi\|85095053	Neurospora crassa	1.40E+00
65I3	EG374375	1870	F	Hypothetical protein	gb\|EAK86140.1	Ustilago maydis	1.00E-129
65O15	EG374381	1893	F	Hypothetical protein	gi\|71006255	Ustilago maydis	1.10E+00
66B6	EG374316	1263	F	Hypothetical protein	gb\|EAK81690.1	Ustilago maydis	1.00E-03
66B11a	EG374437	1145	P	Hypothetical protein	AN2903.3^b	Aspergillus nidulans	3.00E-57
66B11b	EG374438	1200	P	Hypothetical protein	FG10782.1^b	Fusarium graminearum	5.00E-49
66C18	EG374327	2043	F	Hypothetical protein	gb\|EAA59593.1	Aspergillus nidulans	2.00E-12
70A3	EG374360	1835	F	Hypothetical protein	SS1G_14513.1^b	Sclerotinia sclerotiorum	8.00E-18
70C17b	EG374442	1191	P	Hypothetical protein	AN0768.3^b	Aspergillus nidulans	1.00E-07
70H16	EG374426	1121	F	Hypothetical protein	gi\|38100779	Magnaporthe grisea	2.60E+00
70I19b	EG374443	1190	P	Hypothetical protein	NCU02808.2^b	Neurospora crassa	2.00E-08
70K15b	EG374320	933	P	Hypothetical protein	gi\|58261561	Cryptococcus neoformans	1.00E-07
70L24b	EG374446	1168	P	Hypothetical protein	gb\|EAA28928.1	Neurospora crassa	3.00E-23
80I9	EG374394	1060	F	Hypothetical protein	gi\|58259618	Cryptococcus neoformans	1.50E+00
90O3	EG374410	1725	F	Hypothetical protein	gi\|85119288	Neurospora crassa	1.20E-02
90O18	EG374411	1973	F	Hypothetical protein	CHG04543.1^b	Chaetomium globosum	4.00E-07
66C24b	EG374440	1271	P	Macrofage activating glycoprotein	gi\|15722495	Cryptococcus neoformans	3.00E-08
30E3	EG374335	1406	F	Probable gEgh 16 protein	emb\|CAE85538.1	Neurospora crassa	8.00E-07
60I8	EG374355	1039	F	Related to ars binding protein 2	gi\|18376044	Neurospora crassa	6.60E+00
55J15b	EG374285	896	P	Telomeric sequence DNA	gi\|173051	Saccharomyces cerevisiae	2.00E-05
55E7	EG374477	1253	F	Unknown protein in chromosome E	gi\|49654999	Debaryomyces hansenii	3.00E-06
55F15a	EG374281	461	P	Unknown protein in chromosome G	gi\|50427978	Debaryomyces hansenii	2.00E-03
60L20	EG374361	1646	F	Unknown protein in chromosome VI	gi\|39975020	Magnaporthe grisea	3.00E-18
60N1	EG374362	2024	F	Unknown protein in chromosome 1	gi\|46110618	Gibberella zeae	2.00E-09
70F20	EG374415	1818	F	Unknown protein in chromosome III	gi\|58270250	Magnaporthe grisea	1.60E+00
80M4	EG374396	1985	F	Unknown protein in chromosome G	gi\|49657202	Debaryomyces hansenii	1.00E-03
80N10	EG374430	563	P	Phytochrome	gi\|57337632	Emericella nidulans	4.30E-00
90B8	EG374400	2011	F	Unknown protein in chromosome G	gi\|49657202	Debaryomyces hansenii	4.90E-02
90L21	EG374408	2002	F	Unknown protein in chromosome A	gi\|49524079	Candida glabrata	1.20E+00

^a F = full-length sequence and P = partial sequence.
^b Data generated from Blastx search of the fungal database of the Broad Institute [34].

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ISSN: 1471-2164

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