Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions

BMC Genomics

Table 3 Summary of all the P. pastoris strains used in this work.

Parental strain	Product genes	Engineered folding factor	Abbreviation
X33			wild type (wt)
GS115		HAC1	wt+Hac1
SMD1168		HAC1
SMD1168	2F5 Fab		Fab (control)
SMD1168	2F5 Fab	HAC1	Fab+Hac1
SMD1168	2F5 Fab	PDI1	Fab+Pdi
X33	2F5 Fab		for chemostat

GS115 is a histidin auxotrophic P. pastoris strain, X33 is a histidin prototrophic revertant, and SMD1168 is deleted for the vacuolar protease pep 4. All expressing strains carry both the 2F5 Fab light chain gene and the heavy chain fragment under control of the GAP promoter, with the S. cerevisiae mating factor alpha leader as secretion signal. The folding factor engineered strains were transformed with the S. cerevisiae PDI1 or the induced form of Hac1p under control of the GAP promoter within the HIS4 locus.

ISSN: 1471-2164