Table 3 Selected Opsithorchis viverrini contigs that encode families of secreted/membrane proteins that potentially interact with or are exposed to host tissues. Genera of the closest homologues from BLAST × (nr) searches are shown. Where the closest homologue was from a vertebrate (bold font), a tBLASTx search against dbEST was conducted.
From: Gene discovery for the carcinogenic human liver fluke, Opisthorchis viverrini
Predicted function | Examples/comments and genera of closest orthologues/paralogues | Contigs | %identities |
|---|---|---|---|
TGF-β receptor | bone morphogenic protein receptor type I (Sus nr/ Macaca est) | OvAE22 | 44 |
Seven transmembrane receptor | DC-STAMP (Strongylocentrotus); laminin receptor (Bos nr/ Clonorchis est) | OvAE92, OvAE1722 | 51 |
Tetraspanin | stabilize cell membranes – expressed in the tegument of schistosomes (Schistosoma) | OvAE953 | 34 |
C1 family papain-like cysteine protease | cathepsin L (Paragonimus), cathepsin B (Fasciola; Clonorchis) | OvAE1795, OvAE813, OvAE1171, OvAE532, OvAE1070, OvAE1613, OvAE1711, OvAE615, OvAE398 | > 80 |
C13 family asparaginyl endopeptidase | legumain (O. viverrini) | OvAE1624, OvAE1824 | 94 |
S1 family serine protease | HtrA-like (Macaca nr/ Schistosoma est) and kallikrein-like (Schistosoma) peptidases | OvAE622, OvAE1918 | 47–53 |
A1 family aspartic protease | cathepsin D-like; digestive enzyme in helminths (Clonorchis) | OvAE1300 | 80 |
M41 family metalloprotease | mitochondrial membrane proteinase (Schistosoma) | OvAE65 | 91 |
Granulin | mitogen associated with cancer (Bos nr/ Clonorchis est) | OvAE1732 | 45 |
Aquaporin | water channel protein (Schistosoma) | OvAE6 | 48 |
Tyrosinase | critical for S. mansoni egg shell production (Schistosoma) | OvAE1900, OvAE1854 | 63 |
Phospholipase A2 | similar to vertebrate venom proteins; (Heloderma nr/ Clonorchis est) | OvAE1644 | 55 |
Thioredoxin peroxidase | immunomodulatory in fasciolosis (Schistosoma) | OvAE54 | 74 |
EF-hand secreted Ca2+-binding protein | calumenin (Rattus nr/ Xenopus est) | OvAE61 | 47 |
Saposin-like protein | pore forming; similar to fluke cytolysins (Clonorchis) | OvAE1692 | 64 |
Pathogenesis related protein | similar to helminth venom allergen homologues (Schistosoma) | OvAE534, OvAE1862 | 38 |
Glutathione-S-transferase | detoxification of heme and free radicals (Clonorchis) | OvAE1057, OvAE1892, OvAE1601, OvAE1729 | 86 |
Synaptobrevin | neurotransmission/vesicular docking – vesicle associated (Schistosoma) | OvAE1001 | 73 |
Innexin | integral membrane protein forming gap junctions (Schistosoma) | OvAE631 | 78 |
Fibroblast growth factor (FGF) receptor substrate 2 | host FGF is essential for growth of schistosomes (Schistosoma) | OvAE1563 | 32 |
Ly6c | Immune cell differentiation antigen | OvAE82 | 26 |