Figure 2

Functional relationships among the differentially expressed genes were generated using Ingenuity Pathways Analysis. Direct interactions are shown by solid lines and indirect interactions are depicted by broken lines. Red and green colors indicate up- and downregulation in CONV versus GF epithelia, respectively, whereas no color indicates that nodes were not differentially expressed on the array. The network is showing direct literature-supported relationships and confirms the interferon-mediated activation of transcription factors STAT1 and STAT2, which are involved in the expression of IFN-inducible target genes such as class I MHC (HLA-B in figure; pan SLA I on array and by qRT-PCR), B2M, TAP1, TAPBP, and SOCS3 in CONV compared with GF piglets.