Figure 6

Complementation of folC function by Chlamydia trachomatis CT611. Complementation of E. coli folC mutant SF4 by a pBAD24 plasmid harboring CT611 from Chlamydia trachomatis on MS minimal medium with or without glycine plus methionine. E. coli folC was included as a positive control. 1, pCA24N::EcfolC; 2, pBAD24::CT611; 3, pBAD24 alone. SF4 shows slower growth even in the presence of the added amino acids. The appropriate antibiotics and inducers were included in the media as indicated. Amp, ampicillin; Ara, arabinose; Cm, chloramphenicol.