Figure 5

Subcellular localization of the proteins in GC-2 Cells. To determine the subcellular localization of proteins encoded by the 24 genes, GC-2 cells were transfected with each cDNA-GFP in turn. Among these, 14 proteins coded by genes were expressed in GC-2 cells. The figure depicts the localization of these 14 genes. Transfected cells were stained with Hoechst 33258 dye (blue), endoplasmic reticulum marker antibody (antibody to protein disulfide isomerase, red) and Golgi apparatus marker (NBD C₆-ceramide, red). H, Hoechst; ER, endoplasmic reticulum. Scale bar = 10 μm.