Figure 7

Immunofluorescence analysis of the four proteins. (A) Immunofluorescence staining of paraffin sections of adult mouse testis was performed using specific antibodies for the four proteins (shown at the left). Normal serum was used as the negative control. The red color indicates proteins, and blue color represents nucleus staining by Hoechst. The white rectangular boxes are magnified in the insets in merged images. As a further control, the antibodies were pre-treated with the corresponding antigens and used for immunofluorescence staining (shown at the right). This resulted in the disappearance of the signals in spermatogenic cells. Ab, specific antibody to the proteins; Hoechst, Hoechst staining; Merge, merged images between Ab and Hoechst; Ab-Ag, antibodies pre-treated with the corresponding antigens. (B and C) Sperm immunofluorescence using antibodies to two proteins. Mature sperm from cauda epididymis were fixed, pemeabilized and incubated with anti-Mm.333010 (B) or anti-Mm.23534 (C) antibody. a, transmission image; b, fluorescence image; c, Hoechst staining; d, merged image between b and c.