Microarray amplification bias: loss of 30% differentially expressed genes due to long probe – poly(A)-tail distances

Table 3 Primers of 20 selected differentially expressed genes between tumor and bronchus, used for validation with qRT-PCR

Gene	Accession number	Primers (5'→3')	Product (bp)
SOST	NM_025237	FW-CGCTGCCCATCAGAAAGC REV- CAGGACTAGAAACCACATCTACAGTTG	76
MCM6	NM_005915	FW-GGACCTTTCTTATAGGCTGGTCTTTC REV- GCTCTTTCCCCCCAAACCT	74
SDC2	NM_002998	FW-GAGTGTATCCTATTGATGACGATGACTAC REV- CTCTGGACTCTCTACATCCTCATCAG	77
CAPS	NM_004058	FW-AGGTCACACTGGCGGAATTC REV- GGTCATCATGGCCACGAACT	86
B2M	NM_004048	FW-GAAAAAGTGGAGCATTCAGACTTG REV- ATGATGCTGCTTACATGTCTCGAT	174
NME5	NM_003551	FW-AACTCTGCTTGAAGGACTCACAGA REV- CAGCCAATCAGCTAGCCAAAT	76
DCN	NM_001920	FW-GCTGTCAATGCCATCTTCGA REV- GGGAAGATCCTTTGGCACTTT	71
NS3TP2	NM_023927	FW-TGCTTCCTGAGGCGTTTTG REV- CATGCAATGTCTGGATTCTCATC	76
GSTA1	NM_145740	FW-GCTACTTCCCTGCCTTTGAAAA REV- GCCCGGCTCAGCTTGTT	76
UBXD3	NM_152376	FW-GCCAAGGGACGGACAAGAC REV- AGGCGGAGATGGTATATGATGAG	78
SLC1A6	NM_005071	FW-CTTCAAACAGTTCAAGACGCAGTAC REV- CGGCTCAGACCCGTTCTCT	79
PTHLH	NM_002820	FW-CCGC4CTCAAAAGAGCT715GTGT REV- CGCCGTAAATCTTGGATGGA	72
DKK3	NM_013253	FW-AGGAGCCACGAGTGCATCA REV- GGTGTACTGGAAGCTGGCAAAC	75
CGN	NM_020770	FW-TGAGGAATTCGACAGTGTCTACGA REV- GGTCTGTAGGTTGCTCTCCGTAA	70
IL18	NM_001562	FW-AGGAACCTCAGACCTTCCAGATC REV- CTACTGGTTCAGCAGCCATCTTT	81
PTGFR	NM_000959	FW-GGCCTGGGATGACAAGATGT REV- TTGTGGAGATAAAAGCCAACCA	86
CCND1	NM_053056	FW-CCGTCCATGCGGAAGATC REV- CCTCCTCCTCGCACTTCTGT	69
DAF	NM_000574	FW-TGCCCTAATCCGGGAGAAA REV- GAAGGAGATGGTTGCACCAAA	78
KRT7	NM_005556	FW-GCCACCACCCACAATCACA REV- CTTTCCAGACTGTCTCACTGTCTTG	79
NCOA7	NM_181782	FW-TATGTGGCGGGCACCTGTA REV- CTCTGCTTCCCGGATTCAAG	75
RPII	Housekeeping gene	FW- CGTACGCACCACGTCCAAT REV- CAAGAGAGCCAAGTGTCGGTAA	139

ISSN: 1471-2164