Figure 2
From: Biomarker discovery for colon cancer using a 761 gene RT-PCR assay
Effect of increasing priming complexity reverse transcription using FPE tissue RNA template. Eight GSP pools containing from 94 to 96 unique primers were prepared. Two pools were selected at random to prime separate RT-PCR reactions using RNA from FPE tissue. The 8 GSP pools were then combined to make a single GSP pool that was used to prime one RT-PCR reaction using the same FPE RNA template. The data therefore represents concordance between primings for the subset of gene assays represented with those two GSP pools. Both priming methods were performed twice and the average CT values determined for this analysis. The solid line represents the least squares line fit and the dashed line represents the line of concordance.