Table 1 Direction and fold change of genes that establish cell polarity and bipolar budding in yeast cultured under simulated microgravity.
From: Yeast genomic expression patterns in response to low-shear modeled microgravity
Gene | Genbank ID | Fold Change 5 generations v. ctrl | Fold Change 25 generations v. ctrl | Description |
|---|---|---|---|---|
Genes in the Purevdorj-Gage study [13] | ||||
RAX1 | YCR301W | 5.3 ↓ | 5.1 ↓ | bipolar budding |
RAX2 | YLR084C | 3.2 ↓ | 3.4 ↓ | bipolar budding |
DSE1 | YER124C | 8.6 ↓ | 6.0 ↓ | cell separation |
DSE2 | YHR143W | 3.1 ↓ | 3.1 ↓ | cell separation |
BUD5 | YCR038C | 1.7 ↑ | 1.6 ↑ | polarity |
BUD25 | YER014C-A | 1.6 ↑ | 1.7 ↑ | bud site selection |
EGT2 | YNL327W | 7.7 ↓ | 6.1 ↓ | cell separation |
Bipolar budding pattern genes identified in Ni and Snyder study [19] | ||||
SLG1 | YOR008C | 2.2 ↑ | 1.6 ↑ | cell wall integrity, stress response |
VMA5 | YKL080W | 2.1 ↓ | 1.5 ↓ | transport |
PRS3 | YHL011C | 2.2 ↓ | 2.0 ↓ | regulation of cell size |
BUD21 | YOR078W | 2.3 ↓ | 2.6 ↓ | processing |
SUR4 | YLR372W | 2.4 ↓ | 2.5 ↓ | lipid metabolism |
RSR1 | YGR152C | 2.5 ↓ | 2.0 ↓ | bud site selection, cell polarity |
RSP18B | YML026C | 2.6 ↓ | 3.1 ↓ | ribosomal protein |
RPL22A | YLR061W | 3.8 ↓ | 4.4 ↓ | ribosomal protein |
FEN1 | YCR034W | 4.1 ↓ | 3.2 ↓ | lipid metabolism |
NSR1 | YGR159C | 5.6 ↓ | 7.5 ↓ | nuclear protein |
GAS1 | YMR307W | 1.8 ↓ | 2.0 ↓ | cell wall |
BUD22 | YMR014W | 1.9 ↓ | 2.0 ↓ | bud site selection |
BUD23 | YCR047C | 1.8 ↓ | 2.4 ↓ | bud site selection |
STO1 | YLR287C | 2.1 ↓ | 1.7 ↓ | protein biosynthesis |
--- | YLR287C | 2.5 ↓ | 2.4 ↓ | hypothetical ORF |
MIPS functional category: budding and cell polarity | ||||
ERV15 | YBR210W | 1.8 ↑ | 1.8 ↑ | Axial bud site selection |
BUD5 | YCR038C | 1.7 ↑ | 1.6 ↑ | bud site selection |
TWF1 | YGR080W | 1.5 ↑ | 1.6 ↑ | bipolar bud site selection |
BUD13 | YGL174W | 1.4↑ | 1.4 ↑ | bud site selection |
BUD20 | YLR074C | 1.2 ↑ | 1.1 ↑ | bud site selection |
MUB1 | YMR100W | 1.1 ↑ | 1.2 ↑ | regulation of cell budding |
BUD27 | YFL023W | 1.1 ↑ | 1.2 ↑ | bud site selection |
AXL1 | YPR122W | 1.1 ↑ | 1.1 ↑ | bud site selection |
THP1 | YOL072W | 1.1 ↑ | 1.2 ↑ | bud site selection |
LAS1 | YKR063C | 1.1 ↑ | 1.2 ↑ | budding cell bud growth |
STE20 | YHL007C | 1.0 ↓ | 1.0 ↓ | bud site selection |
BUD16 | YEL029C | 1.1 ↓ | 1.0 ↓ | bud site selection |
BUD7 | YOR299W | 1.2 ↓ | 1.1 ↑ | bud site selection |
BUD2 | YKL092C | 1.2 ↓ | 1.1 ↓ | bud site selection |
BUD8 | YLR353W | 1.2 ↓ | 1.3 ↓ | bud site selection |
BUD32 | YGR262C | 1.2 ↓ | 1.3 ↓ | bud site selection |
BUD17 | YNR027W | 1.2 ↓ | 1.0 ↓ | bud site selection |
RRP14 | YKL082C | 1.3 ↑ | 1.4 ↓ | cell polarity |
BUD3 | YCL014W | 1.3 ↓ | 1.4 ↓ | bud site selection |
BUD31 | YCR063W | 1.4 ↓ | 1.3 ↓ | bud site selection |
BUD6 | YLR319C | 1.4 ↓ | 1.4 ↓ | bud site selection |
AXL2 | YIL140W | 1.4 ↓ | 1.4 ↓ | bud site selection |
HKR1 | YDR420W | 1.5 ↓ | 1.7 ↓ | bud site selection |
BUD9 | YGR041W | 1.6 ↓ | 1.9 ↓ | bud site selection |
TOS2 | YGR221C | 1.6 ↓ | 1.7 ↓ | budding cell bud growth |
BUD23 | YCR047C | 1.8 ↓ | 2.4 ↓ | bud site selection |
BUD22 | YMR014W | 1.9 ↓ | 2.0 ↓ | bud site selection |
RSR1 | YGR152C | 2.5 ↓ | 2.0 ↓ | bud site selection |
BUD4 | YJR092W | 2.7 ↓ | 2.0 ↓ | bud site selection |
RAX2 | YLR084C | 3.2 ↓ | 3.2 ↓ | bud site selection |
RAX1 | YOR301W | 5.3 ↓ | 5.1 ↓ | bud site selection |