Exploring the midgut transcriptome of Phlebotomus papatasi: comparative analysis of expression profiles of sugar-fed, blood-fed and Leishmania major-infected sandflies

Table 1 List of Phlebotomus papatasi midgut-specific sequences, clusters, and sequences per cluster of cDNA libraries made from flies sugar-fed, blood-fed, and blood fed with Leishmania major parasites

	Number of Clusters			Number of Sequences			Sequences/cluster
Biological function	Sugar	Blood-fed	L. major	Sugar	Blood-fed	L. major	Sugar	Blood-fed	L. major
protein synthesis machinery	88	39	51	281	73	159	3.19	1.87	3.12
protein modification machinery	19	20	8	39	22	9	2.05	1.10	1.13
protein export machinery	14	13	10	14	13	10	1.00	1.00	1.00
transcription machinery	4	2	1	4	2	1	1.00	1.00	1.00
transcription factors	13	6	3	22	9	4	1.69	1.50	1.33
proteasome machinery	13	7	7	21	7	9	1.62	1.00	1.29
transporters	24	16	11	37	19	33	1.54	1.19	3.00
extracellular matrix	6	12	5	164	122	59	27.33	10.17	11.80
cytoskeletal	16	15	14	61	291	210	3.81	19.40	15.00
signal transduction	28	20	13	38	20	15	1.36	1.00	1.15
protease inhibitor	3	1	3	8	5	9	2.67	5.00	3.00
immunity	3	4	3	8	5	4	2.67	1.25	1.33
adhesion	1	2	1	1	2	1	1.00	1.00	1.00
nuclear metabolism and regulation	17	7	6	18	9	6	1.06	1.29	1.00
metabolism, energy	95	38	42	194	54	62	2.04	1.42	1.48
metabolism, lipid	9	11	9	11	18	10	1.22	1.64	1.11
metabolism, carbohydrate	16	9	10	27	12	14	1.69	1.33	1.40
metabolism, amino acid	28	40	30	131	182	197	4.68	4.55	6.57
metabolism, nucleic acid and nucleotides	7	9	4	14	9	4	2.00	1.00	1.00
metabolism, heme	3	3	2	8	28	8	2.67	9.33	4.00
conserved of unknown function	262	167	322	405	282	470	1.55	1.69	1.46
Total	669	441	555	1506	1184	1294	Avg 3.23	Avg 3.27	Avg 3.01

ISSN: 1471-2164