Skip to main content
Figure 3 | BMC Genomics

Figure 3

From: Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region

Figure 3

The minimum BAC tiling pattern of giant panda MHC class II region. A total of 11 BAC clones were involved: 504E11 (67 kb), 237D4 (84 kb), 354C3 (69 kb), 826G2 (50 kb), 606E7 (110 kb), 206G5 (73 kb), 900H8 (85 kb), 692B2 (35 kb), 1561B8 (45 kb), 1262B6 (110 kb) and 186A7 (54 kb). Ten MHC class II primerpairs (BTNL2, DRA, DRB, DQA, DOB, LMP2, DMB, DMA, COL11A2 and DAXX), eight end primerpairs marked with black triangle (237D4-T7, 237D4-RP2, 826G2-T7, 826G2-RP2, 206G5-T7, 900H8-T7, 692B2-RP2 and 186A7-T7) and two shotgun-sequence primerpairs (692B2-F8R8 and 1262B6-F5R5) marked with blank triangle were anchored to the contig. This BAC contig map of panda MHC class II region spans about 650 kb. The location of genes was determined against the human, dog, cat and other mammalian MHC class II loci. Star symbols indicate the MHC genes are probably multiple loci but this study utilizes one-locus-amplified primers.

Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us